بررسی تأثیر تنظیم‌کننده‌های رشد و امواج فراصوت بر ریشه‌زایی و پینه‌زایی سوسن چلچراغ (Lilium ledebourii Bioss.) در شرایط درون‌شیشه‌ای

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکده کشاورزی، دانشگاه محقق اردبیلی

2 استادیار، دانشکده کشاورزی، دانشگاه محقق اردبیلی

3 دانشیار، دانشکده کشاورزی، دانشگاه محقق اردبیلی

چکیده

این تحقیق به‌منظور بررسی تأثیر تیمار NAA و BA و امواج فراصوت بر ریشه‌زایی و پینه (کالوس‌)زایی ریزنمونه‌های سوسن چلچراغ (Lilium ledebourii Bioss.) در شرایط درون‌شیشه‌ای انجام شد. برای این منظور ریزنمونه‌های فلس پس از قرارگیری در حمام فراصوت با بسامد (فرکانس) 35 کیلوهرتز با زمان‌های 0، 5، 10، 20 و 30 ثانیه، در محیط کشت MS حاوی غلظت‌های 0، 01/0، 1/0 و 1 میلی‌گرم در لیتر NAA و BA به‌تنهایی و یا به‌صورت ترکیب با یکدیگر کشت شد. این تحقیق در چهار آزمایش جداگانه به ترتیب شامل ترکیب NAA و BA، NAA و فراصوت، BA و فراصوت و بهترین تیمارهای آزمایش اول همراه با فراصوت انجام گرفت. در آزمایش اول و دوم تیمار mg l-1 1 NAA بیشترین شمار ریشه و طول ریشه را داشت و در آزمایش سوم تیمار شاهد نتیجۀ بهتری در این زمینه داشت. در آزمایش دوم فراصوت ریشه‌زایی را تحریک نکرد، بااین‌حال در آزمایش سوم، در غلظت پایین BA فراصوت تأثیر مثبتی در ریشه‌زایی داشت و شمار ریشه‌های به‌دست‌آمده را تا 12-10 عدد افزایش داد، ولی در غلظت 0 و mg l-1  BA 1، تأثیر مثبتی در ریشه‌زایی نداشت. نتایج مربوط به پینه‌زایی نشان داد، تنظیم‌کننده‌های رشد و امواج فراصوت تأثیر معنی‌داری بر میزان پینه‌زایی ریزنمونه نداشتند. در این پژوهش غلظت mg l-11 NAA به‌تنهایی بهترین تیمار برای افزایش فراسنجه (پارامتر)های مربوط به ریشه‌زایی ریزنمونۀ فلس شناخته شد.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of plant growth regulators and ultrasound treatments on in vitro rooting and callogenesis of Lilium ledebourii Boiss.

نویسندگان [English]

  • Zahra Azimzadeh 1
  • Mehdi Mohebodini 2
  • Esmaeil Chamani 3
1 Former M. Sc. Student, Faculty of Agriculture, Mohaghegh Ardabili University, Ardabil, Iran
2 Assistant Professor, Faculty of Agriculture, Mohaghegh Ardabili University, Ardabil, Iran
3 Assocaite Professor, Faculty of Agriculture, Mohaghegh Ardabili University, Ardabil, Iran
چکیده [English]

This study was performed to evaluate the effect of NAA, BA and ultrasound waves on in vitro rooting and callus induction of Lilium ledebourii. For this purpose, scale explants after exposure to an ultrasonic bath with a frequency of 35 kHz in times 0, 5, 10, 20 and 30 seconds were cultured on MS medium containing different concentrations (0, 0.01, 0.1 and 1 mg l-1) of NAA and BA alone or in combination with each other. This study was carried out in four experiments including combination of NAA and BA, NAA and ultrasound, BA and ultrasound, and the best treatments which achieved from first experiment with ultrasound, respectively. In the first and second experiment, 1 mg l-1 NAA treatments had the highest root induction and in the third experiment control treatment had the better result. In the second experiment, ultrasound did not stimulate root induction, however, in the third experiment, in 0 and 1 mg l-1 BA concentrations, ultrasound had no positive effect on root induction, but at low concentrations, BA increased the number of roots. Moreover, it was indicated that plant growth regulators and ultrasound had no significant effect on callus induction. In this study, concentration of 1 mg l-1 NAA was the best treatment for increasing of rooting parameters in scale explants.

کلیدواژه‌ها [English]

  • BA
  • NAA
  • explant
  • Scale
  1. Ananthakrishnan, G., Xia, X., Amutha, S., Singer, S., Muruganantham, M., Yablonsky, S., Fischer, E. & Gaba, V. (2007). Ultrasonic treatment stimulates multiple shoot regeneration and explant enlargement in recalcitrant squash cotyledon explants in-vitro. Plant Cell Reports, 26, 267-276.
  2. Azadi, P. & Khosh-Khui, M. (2007). Micropropagation of Lilium ledebourii (Baker) Boiss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatments. Electronic Journal of Biotechnology, 4, 582-591.
  3. Azadi, P. & Mojtahedi, N. (2010). Effect of growth regulators sucrose concentration and scale segment on micropropagation of Lilium ledebourii in winter harvested bulbs. Pazhohesh and Sazandeghi in Agronomy and Horticulture, 81, 154159. (in Farsi)
  4. Bageri, A. & Saffari, M. (2007). Principles of plant tissue culture. Mashhad: publications of Mashhad Ferdowsi University. (in Farsi) 
  5. Bina, F. & Zamani, Z. (2009). Investigation of the effect of ultrasound and various concentration of auxin (IBA) on rooting rose) Rosa damascena Mill.) cuttings. In: Proceedings of 6th Congress of Iran Horticultural Science, Gilan University, pp. 1059-1061. (in Farsi)
  6. Bochu, W., Jiping, S., Biao, L., Jie, L. & Chuanren, D. (2004). Soundwave stimulation triggers the content change of the endogenous hormone of the Chrysanthemum mature callus. Colloids and Surfaces B: Biointerfaces, 37, 107-112.
  7. Chang, C., Chen, C. T., Tsai, Y. C. & Chang, W. C. (2000). A tissue culture protocol for propagation of a rare plant, Lilium speciosum Thunb. Var. Gloriosoides Baker. Botanical Bulletin of Academia Sinica, 41, 139-142.
  8. El-Naggar, H., Osman, A. & Sewedan, E. (2012). In-vitro propagation and organogenesis of Lilium ‘Prato’. African Journal of Biotechnology, 82, 14771-14776.
  9. Farsam, H., Amanlou, M., Amin, G., Nezamivand- Chegini, G., Salehi- Surmaghi, M. H. & Shafiee, A. (2003). Anatomical and phytochemical study of Lilium ledebourii (Baker) Boiss; a rare endemic spesies in Iran. DARU Journal, 4, 164-170.
  10. Gaba, V., Kathiravan, K., Amutha, S., Singer, S., Xiaodi, X. & Ananthakrishnan, G. (2006). The uses of ultrasound in plant tissue culture. Plant Tissue Culture Engineering, 417-426.
  11. Izadi, N., Mashayekhi, K., Chamani, E., Kamkar, B. (2001). The influence of B5 basal medium on morphological behavior of Lily (Lilium longiflorum) bulbscale in-vitro. Plant Production, 18(1), 119- 132. (in Farsi) 
  12. Kanchanapoom, K., Ponpiboon, T., Wirakiat, W. & Kanchanapoom, K. (2011). Regeneration of lily (Lilium longiflorum ‘Easter lily’) by callus derived from leaf explants cultured in-vitro. Science Asia, 37,373-376.
  13. Kapoor, R., Kumar, S. & Kanwar, J. K. (2009). Bulblet production from node explant grown in-vitro in hybrid lilies. International Journal of Plant Production, 3, 1-6.
  14. Khavar, K. M., Cocu, S., Parmaksiz, I., Sarihan, E. O. & Ozcan, S. (2005). Mass proliferation of Madonna Lily (Lilium candidum L.) under in-vitro coditions. Pakistan Journal Botany, 2, 243-248.
  15. Lamberova, M. E., Khmeleva, A. N., Emelianova, I. S., Lamberova, A. A. & Kosolapova, A. S. (2006). Researching of ultrasonic influence to sterilization and plants cells growth of culture in-vitro. Electron Devices and Materials, 294-296.
  16. Liu, R., Li, W., Sun, L. Y. & Liu, C. Z. (2012). Improving root growth and cichoric acid derivatives production in hairy root culture of Echinacea purpurea by ultrasound treatment. Biochemical Engineering Journal, 60, 62-66.  
  17. Liu, Y., Takatsuki, H., Yoshikoshi, A., Wang, B. & Sakanishi, A. (2003a). Effects of ultrasound on the growth and vacuolar H+-ATPase activity of Aloe arborescens callus cells. Colloids and Surfaces B: Biointerfaces, 32, 105-116.
  18. Liu, Y., Yoshikoshi, A., Wang, B. & Sakanishi, A. (2003b). Influence of ultrasonic stimulation on the growth and proliferation of Oryza sativa Nipponbare callus cells. Colloids and Surfaces B: Biointerfaces, 27, 287-293.
  19. Marinangeli, P. A., Hernandez, L. F., Pellegrini, C. P. & Curvetto, N. R. (2003). Bulblet differentiation after scale propagation of Lilium longiflorum. Journal of the American Society for Horticultural  Science, 3, 324-329.
  20. Memar Moshrefi, M, Moini, A. & Tavasolian, I. (2002). Effects of plant growth regulators NAA, BAP. Different explants scale photoperiod on tissue culture of Lilium ledebourii Boiss. Journal of Iran Crop Sciences, 4(4), 261-253. (in Farsi)
  21. Miller, D. L. (1983). The botanical effects of ultrasound: A review. Environmental and Experimental Botany, 1, 1- 27.
  22. Mir, J. I., Ahmed, N., Itoo, H., Sheikh, M. A., Rashid, R. & Wani, S. H. (2012). In-vitro  propagation of Lilium (Lilium longiflorum). Indian Journal of Agricultural Sciences, 82, 455-558.
  23. Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with Tobacco tissue cultures. Journal of Plant Physiology, 15, 473-497.
  24. Naseri, M. T. & Ebrahimi Garvi, M. (1998).  Physiology bulbous flowers. Mashhad: publications of Mashhad Ferdowsi University. (in Farsi)
  25. Nhut, D. T. (1998). Micropropagation of lily (Lilium longiflorum) via  in-vitro stem node and pseudobulblet culture. Plant Cell Reports, 17, 913-916.
  26. Padasht Dahkaei, M. N., Khalighi, A., Naderi, R. & Mousavi, A. (2008). Effect of different concentrations of benzyladenine (BA) and naphthalene acetic acid (NAA) on regeneraton of Lilium ledebourii (Chelcheragh Lily) using bulblets microscales. Seed and Plant Improvement Journal, 24(2), 321-331. (in Farsi)
  27. Robert Ault, J. & Siqueira, S. S. (2008). Morphogenetic response of Lilium michiganense to four auxin type plant growth regulators in-vitro. Horticultural Science, 43, 1922-1924.
  28. Saifulah, K., Sheeba, N., Mariam, R., Naheed, K., Asma, N. & Bushra, S. (2010). Cultivation of lilies (Lilium regale) for commercialization in Pakistan. Pakistan Journal Botany, 42, 1103-1113.
  29. Tang, Y. P., Liu, X. Q., Wahiti Gituru, R. & Chen, L. Q. (2010). Callus induction and plant regeneration from in-vitro cultured leaves, petioles and scales of Lilium leucanthum (Baker) Baker. Biotechnology and Biotechnological Equipment, 24, 2071-2076.
  30. Tatari Varnusfaderani, M., Fotouhi  Ghazvini, R., Hamidoghli, Y. & Hatamzadeh, A. (2007). Effects of plant growth regulators and kind of explant on in-vitro culture of  scales of Chelcheragh lily (Lilium ledebourii Bioss.). Journal of Iran Agricultural Science, 38(2), 276-284. (in Farsi)
  31. Teixeira da Silva, J. A., Tran Thanh Van, K., Biondi, S., Nhut, D. T. & Maddalena Altamura, M. (2007). Thin cell layers: developmental building blocks in ornamental biotechnology. Floriculture and Ornamental Biotechnology, 1, 1-13.
  32. Torang, A. & Magsodi, M. (2012). Optimize medium for in-vitro callus production and regeneration of lily plantlets (Lilium spp.). The first National Conference Biotechnology, Gorgan, Golestan University, http://www.civilica.com/Paper-NSCBIO01-NSCBIO01_152.html. (in Farsi)
  33. Wawrosch, C., Malla, P. R. & Kopp, B. (2001). Clonal propagation of Lilium nepalense D. Don, a threatened medicinal plant of Nepal. Plant Cell Reports, 20, 285-288.