تأثیر سیلیکات پتاسیم بر ویژگی‌های رشدی، فیزیولوژی و بیوشیمیایی شمعدانی معطر (Pelargonium graveolens) در شرایط تنش شوری

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکدۀ کشاورزی، دانشگاه لرستان

2 دانشیار، دانشکدۀ کشاورزی، دانشگاه لرستان

چکیده

این پژوهش به‌منظور بررسی تأثیر سیلیکات پتاسیم بر واکنش گیاه شمعدانی معطر به تنش شوری در سال 1393 انجام شد. آزمایش به‌صورت گلدانی، آبکشتی (هیدروپونیک) درون ماسه و بر پایۀ فاکتوریل در قالب طرح کامل تصادفی شامل چهار واحد آزمایشی و هر واحد شامل 9 ترکیب تیماری و درمجموع 36 گلدان انجام شد. عامل‌ها شامل شوری ناشی از کلرید‌سدیم با ایجاد سه سطح هدایت الکتریکی 8/1 (ناشی از محلول غذایی به‌عنوان شاهد، بدون کلرید سدیم)، 4 و 6 دسی‌زیمنس ‌بر ‌متر و کاربرد هفتگی سیلیکات پتاسیم در سه سطح 0، 5/0 و 1 میلی‌مولار بود. نتایج نشان دادند، با افزایش شوری، فراسنجه (پارامتر)های رشد گیاه، میزان اسانس، محتوای نسبی آب و فعالیت آنزیم‌های کاتالاز و پراکسیداز کاهش یافت درحالی‌که میزان مالون‌دی‌آلدئید، نشت الکترولیت‌ها و میزان پرولین افزایش یافتند. سیلیکات پتاسیم در بهبود رشد و ویژگی‌های بیوشیمیایی گیاه در شرایط شوری مؤثر بود. همچنین با افزایش سطح شوری میزان سدیم افزایش پیدا کرد و میزان پتاسیم کاهش یافت و کاربرد سیلیکات پتاسیم باعث کاهش سدیم و افزایش پتاسیم  در اندام‌های هوایی گیاه شد. در بیشتر ویژگی‌ها از جمله سطح برگ، وزن تر و خشک گیاه کاربرد سیلیکات پتاسیم با غلظت 1 میلی‌مولار عملکردی همانند به شاهد را نشان داد. به‌طورکلی نتایج نشان داد، افزایش شوری باعث تشدید تنش شده و کاربرد هفتگی سیلیکات پتاسیم به‌ویژه با غلظت 1 میلی‌مولار باعث کاهش تأثیر تنش شد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of potassium silicate on growth, physiological and biochemical characteristics of Pelargonium graveolens under salinity stress

نویسندگان [English]

  • Fatemeh Hassanvand 1
  • Abdolhossein Rezaei Nejad 2
1 Former M.Sc. Student, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
2 Associate Professor, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
چکیده [English]

This research was carried out to evaluate the effect of potassium silicate on geranium reaction to salinity stress in 2014. The experiment was done hydroponically in pots filled with sand. Experiment was laid out factorially based on a completely randomized design with four replications each replication included 9 treatment compositions with 36 pots. Factors consisted of daily application of 1.8, 4 and 6 ds/m NaCl and weekly application of 0, 0.5 and 1 mM potassium silicate through nutrient solution. Result showed that by increasing salinity, growth parameters, essential oil, relative water content and the activity of catalase and peroxidase decreased while Malondialdehyde, electrolyte Leakage and amount of proline increased. Application of potassium silicate improved growth and physiological and biochemical characteristics ofgeranium under salinity stress. Moreover, leaf Na increased while P content decreased, respectively, as salinity increased and application of potassium silicate increased P and decreased Na under salinity in canopy of plant. Many characters i.e. leaf area, shoot fresh and dry weights values returned to control as 1mM potassium silicate was applied. Overall, the results showed that increasing salinity pronounced stress symptoms and weekly application of potassium silicate especially at 1 mM, alleviated the stress effects.

کلیدواژه‌ها [English]

  • Catalase
  • Essential oil
  • potassium
  • proline
  • Peroxidase
  • Silicon
  • sodium
  1. Al-aghabary, K., Zhujun, Z. & Qinhua, S. (2004). Influence of silicon supply on chlorophyll content, chlorophyll fluorescence, and antioxidative enzyme activities in tomato plants under salt stress. Journal of Plant Nutrition, 27, 2101-2115.
  2. Bates, L. S., Waldron, R. P. & Teare, I. D. (1973). Rapid determination of free proline for water stress studies. Plant Soil, 39, 205-217.
  3. Buege, J. A. & Aust, S. D. (1978). Microsomal lipid peroxidation. Methods Enzyme, 52, 302-310.
  4. Campbell, C. R. & Plank, C. O. (1998). Preparation of plant tissue for laboratory analysis. In Handbook of reference methods for plant analysis, ed. Y. P. Kalra, 37–49. Boca Raton, Fl.: CRC Press.
  5. Chance, B. & Maehly, A. (1955). Assay of catalase and peroxidase. Methods in Enzymology, 2, 764-817.
  6. Dadras, N., besharati, H. & Ketabchi, S. (2001). Effects of salinity of NaCl on growth and N biological fixation in tree cultivar of glycine. Journal Researches of Soil, 26, 165-174. (in Farsi)
  7. Dirk, I. & Montago, M. V. (2003). Oxidative Stress in Plants. CRC Press.
  8. Evans, W. C. (1996). Pharmacognosy. Volatile Oils and Resins. 14th Edition. John Wiley. New York. 450 pp.
  9. Gunes, A., Kadioglu, Y. K., Pilbeam, D. J., Inal, A., Coban, S. & Aksu, A. (2008). Influence of silicon on sunflower cultivars under drought stress. Communications in Soil Science and Plant Analysis, 39, 1904-1927.
  10. Haghighi, M. & Pessarakli, M. (2013). Influence of silicon and nano-silicon on salinity tolerance of cherrytomatoes (Solanum lycopersicum L.) at early growth stage. Scientia Horticulturae, 161, 111-117
  11. Helal Ragab, M. (2006). Influence of exogenous application of silicon on physiological response of salt-stressed maize (Zea mays L.). International Journal of Agriculture & Biology, 8(2), 293-297.
  12. Jafarzadeh, A. A. & Aliasgharzad, N. (2007). Salinity and salt composition effects on seed germination and root length of four sugar beet cultivars, Proceeding of “Bioclimatology and Natural Hazards” International Scientific Conference, Po_ana nad Detvou, Slovakia, September. 17-20.
  13. Kaya, C., Tuna, L. & Higgs, D. (2006). Effect of silicon on plant growth and mineral nutrition of maize grown under water stress condition. Journal Plant Nutrition, 29, 1469-1480.
  14. Lichtenthaler, H. K. (1987). Chlorophylls and carotenoids: pigments of photosynthetic bio-membranes. In: Method in Enzymol. (eds.s.p.colowick and N.O. Kaplan) Academic press. New York. 48, 350-382.
  15. Liang, Y. (1999). Effects of silicon on enzyme activity and sodium, potassium and calcium concentration in barley under salt stress. Plant Soil, 209, 217-224.
  16. Liang, Y. C., Shen, Q. R. Shen, Z. G. & Ma, T. S. (1996). Effects of silicon on salinity tolerance of two barely cultivars. Journal of Plant Nutrition, 19, 173-183.
  17. Lutts, S., Kinet, J. M. & Bouharmont, J. (1996). NaCl-induced senesence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance, Annals of Botany, 78,389-398.
  18. Ma, J. F. & Takahashi, E. (2002). Soil Fertilizer and plant silicon research in japan. Elsevier. The Netherlands. 281p.
  19. Motsara, M. R. & Roy, R. N. (2008). Guide to laboratory establishment for plant nutrient analysis. 220.
  20. Munns, R. & Tester, M. (2008). Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59, 651-681.
  21. MacAdam, J. W., Nelson, C. J. & Sharp, R. E. (1992). Peroxidase Activity in the leaf elongation zone of tall fescue. Plant Physiology, 99, 872-878.
  22. Mateos-Naranjo, E., Andrade's-Moreno, L. & Davy, A. J. (2013). Silicon alleviates deleterious effects of high salinity on the halophytic grass Spartina densiflora. Plant Physiology and Biochemistry, 63, 115-121.
  23. Marschner, H. (1995). Mineral nutrition of higher plants. (2nd Ed.). Academic Press. London. Pp 674.
  24. Neocleous, D. & Vasilakakis, M. (2007). Effects of NaCl stress on red raspberry (Rubus idaeus L. "Autumn Bliss"). Scientia Horticulturae, 112, 282-289.
  25. Omidbaigi, R. & Rezaei Nejad, A. (2000). The influence of nitrogen-fertilizer and harvest time on the productivity of Thymus vulgaris L. International Journal of Horticultural Science, 6, 43-46.
  26. Rahimi, R., Mohammakhani, A., Roohi, V. & Armand, N. (2012). Effects of salt stress and silicon nutrition on chlorophyll content, yield and yield components in fennel (Foeniculum vulgar Mill.). International Journal of Agriculture and Crop Sciences, 4(21), 1591-1595.
  27. Rajeswara Rao, B. R. (2002). Biomass yield essential oil yield and essential oil composition of rose-scented geranium (Pelargonium species) as influenced by row spacing and intercropping with cornmint (Mentha arvensis L.f. piperascens Malinv. ex Holmes). Industrial Crops and Products, 16, 133-144.
  28. Rezaei Nejad, A. & Ismaili, A. (2014). Changes in growth, essential oil yield and composition of geranium (Pelargonium graveolens L.) as affected by growing media. Journal Science Food Agriculture, 94, 905-910.
  29. Ritchie, S. W. & Hanson, A. D. (1990). Leaf water content and gas exchange parameters of two wheat genotypes differing in drought resistance. Crop Science, 30, 105-111.
  30. Sultan, A. (2005). Effect of NaCl salinity on photosynthesis and dry matter accumulation in developing rice grains. Environmental and Experimental Botany, 42(3), 211-220.
  31. Savvas, D., Giotis, D., Chatzieustratiou, E., Bakea, M. & Patakioutas, G. (2009). Silicon supply in soilless cultivations of zucchini alleviates stress induced by salinity and powdery mildew infections. Environmental and Experimental Botany, 65, 11-17.
  32. Sang, G. K., Ki, W. K., Eun, W. P. & Doil, C. (2002). Silicon-induced cell wall fortification of rice leaves: A possible cellular mechanism of enhanced host resistance to blast. Phytopathology, 92, 1095-1103.
  33. Tala ahmad, S. & Haddad, R. (2010). Effect of silicon on antioxidant enzyme and osmotic regulator in 2 genotype of wheat in drought tolerance. Journal of Nahal & Bazr, 2-26(2), 207-225.(in Farsi)
  34. Vendruscolo, E. C. G., Schuster, I., Pilegg, M., Scapim, C. A., Molinari, H. B. C., Marur, C. J. & Vieira, L. G. E. (2007). Stress-induced synthesis of proline confers tolerance to water deficit in transgenic wheat. Journal of Plant Physology, 164(10), 1367-1376.
  35. Yamaguchi, T. & Blumwald, E. (2005). Developing salt-tolerant crop plants: Challenges and opportunities. Trends in Plant Science, 12, 615-620.
  36. Yamasaki, S. & Dillenburg, L. C. (1999). Measurements of leaf relative water content in Araucaria angustifolia. Revista Brasilian Fisiologia Vegetal, 11, 69-75.
  37. Zuccarini, P. (2008). Effect of silicon on photosynthesis, water relations and nutrient uptake of haseolus vulgaris under NaCl stress, Biologia Plantarum, 52(1), 157-160.