ارزیابی بیان ژن ACO و فعالیت آنزیم کاتالاز تحت تأثیر اسیدسالیسیلیک و عصارۀ گیاهی در دو رقم گل شاخه بریدنی میخک مینیاتوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشگاه علوم کشاورزی و منابع طبیعی ساری

2 استادیار، دانشگاه علوم کشاورزی و منابع طبیعی ساری

چکیده

میخک یکی از مهم‌ترین گل­های شاخه بریدنی جهان است. طول عمر کوتاه این گل باعث کاهش ارزش اقتصادی آن می­شود. به کمک برخی از مواد می­توان ماندگاری گل­های بریدنی را افزایش داد. بنابراین آزمایشی برای افزایش عمر گل­های بریدنی با استفاده از تیمار هورمونی سالیسیلیک اسید و عصارۀ گیاهی انجام شد. این آزمایش به‌صورت فاکتوریل و در قالب طرح کامل تصادفی با ده تیمار (غلظت­های مختلف سالیسیلیک اسید، عصارۀ مرزه و شاهدانه) و چهار تکرار با دو رقم گل بریدنی میخک مینیاتوری انجام شد. عامل‌هایی مانند عمر­گلجایی، آنزیم پاداکسندگی (آنتی­اکسیدانی) کاتالاز و میزان بیان نسبی ژن ACC اکسیداز (ACO) ارزیابی شد. نتایج به‌دست‌آمده نشان داد، اثر متقابل تیمار و رقم در عمر­گلجایی مؤثر بوده و رقم spectro عمر گلجایی بیشتری نسبت به رقم pretty tessino داشت. در تیمار سالیسیلیک اسید ppm150 عمر گلجایی و فعالیت آنزیم کاتالاز بیشتر و بیان نسبی ژن ACO کمتر از دیگر تیمارها و شاهد بود. تیمارهای عصارۀ گیاهی مرزه و شاهدانه در غلظت­های بالا افزون بر تأثیر مثبت در عمر­گلجایی و فعالیت آنزیم کاتالاز، بیان شدن ژن ACO را کاهش داد. بنابراین به نظر می­رسد استفاده از این تیمارها می­تواند مکمل و یا جایگزین مناسبی برای تیمارهای شیمیایی دیگر باشد.

کلیدواژه‌ها

عنوان مقاله [English]

ACO gene expression and catalase activity under the influence of salicylic acid and plant extracts in two miniatures Carnation cut flowers

نویسندگان [English]

  • Mohammad Hassan Heydari Alamdarlou 1
  • Hossein Moradi 2
  • Mahnaz Karimi 2
  • Vali-ollah Ghasemi Omran 2
1 Former M. Sc. Student, Sari Agricultural Sciences & Natural Resources University, Sari, Iran
2 Assistant Professor, Sari Agricultural Sciences & Natural Resources University, Sari, Iran
چکیده [English]

Carnation is one of the most important cut flowers in the world. The flower is to reduce the economic value due to its short vase life. Some materials can be used to help increase the vase life of cut flowers. According to this, using salicylic acid hormone and plant extracts treatment increased the shelf life of cut flowers. Factorial experiment based on a completely randomized design was conducted with 10 treatments (Different concentrations of salicylic acid, Savory and hemp extracts) and four replications with two varieties of miniature carnation. Factors such as vase life, antioxidant enzymes catalase and the relative gene expression ACC oxidase (ACO) were evaluated. Results showed that the interaction of treatments and cultivars was effective in vase life. Spectro cultivar vase life was more than a pretty tessino cultivar. Salicylic acid at 150ppm increased vase life and catalase activity. In addition, this treatment decreased relative ACO gene expression more than the other treatments and control. Savory and hemp extract treatments at high concentrations increased vase life as well as catalase activity, but ACO expression decreased. So it seems that the use of these treatments can be complementary or good alternative to other chemical treatments.

کلیدواژه‌ها [English]

  • Hemp extract
  • Savory extract
  • Salicylic Acid
  • vase life

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سابقه مقاله

  • تاریخ دریافت: 08 اسفند 1394
  • تاریخ بازنگری: 10 مرداد 1395
  • تاریخ پذیرش: 16 شهریور 1395

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