ارزیابی تأثیر سطوح مختلف کیتوزان بر فعالیت آنزیم‌های پاداکسنده در گیاه دارویی زنیان (Carum copticum L.) در شرایط تنش خشکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، گروه باغبانی و فضای سبز، دانشکدۀ کشاورزی، دانشگاه زابل

2 استادیار، گروه باغبانی و فضای سبز، دانشکدۀ کشاورزی، دانشگاه زابل

3 دانشیار، گروه باغبانی و فضای سبز، دانشکدۀ کشاورزی، دانشگاه زابل

4 مربی، گروه باغبانی و فضای سبز، دانشکدۀ کشاورزی، دانشگاه زابل

چکیده

 امروزه کاربرد کیتوزان به‌عنوان یکی از هورمون‌های گیاهی در افزایش مقاومت گیاهان به تنش‌هایی همچون خشکی افزایش‌یافته است. به‌منظور ارزیابی تأثیر سطوح مختلف کیتوزان بر فعالیت آنزیم­های پاداکسنده (آنتی‌اکسیدان) در گیاه دارویی زنیان در شرایط تنش خشکی آزمایشی به‌صورت کرت­های خردشده در قالب طرح بلوک­های کامل تصادفی با سه تکرار در سال 1393 در ایستگاه تحقیقاتی دانشگاه زابل اجرا شد. خشکی به‌عنوان عامل اصلی در سه سطح (A1) 50 میلی­متر تبخیر (شاهد)، (A2)  100 میلی­متر تبخیر (تنش متوسط) و(A3)  150میلی­متر تبخیر (تنش شدید) از تشتک تبخیر کلاس A و سطوح مختلف محلول­پاشی کیتوزان (شاهد، 1/0، 5/0، 1، 2 گرم در لیتر) به‌عنوان عامل فرعی در نظر گرفته شد. در این پژوهش با افزایش دور آبیاری بر میزان فعالیت آنزیم­های کاتالاز، پراکسیداز، آسکوربات پراکسیداز، گایاکول پراکسیداز و پلی فنول اکسیداز نیز افزوده گشت. تیمار شاهد و تنش متوسط در بیشتر فراسنجه (پارامتر)ها اختلاف معنی­داری از نظر آماری نداشتند اما در سطح تنش شدید بیشترین فعالیت آنزیم‌ها مشاهده شد. استفاده از کیتوزان در سطوح مختلف باعث بهبود بخشیدن فعالیت آنزیم­های پاداکسنده شد به‌طوری‌که اثر متقابل سطوح 5/0 گرم در لیتر کیتوزان و تنش خشکی شدید توانست بیشتر فراسنجه‌ها را نسبت به شاهد افزایش قابل توجه دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluate the effect of chitosan on the activities of antioxidant enzymes in Ajwain (Carum copticum L.) under drought stress

نویسندگان [English]

  • Farzad Taheri 1
  • Mehdi Dahmardeh 2
  • Mohammad Salari 3
  • Reza Bagheri 4
1 Former M. Sc. Student, Department of Landscape and Horticultural Science, Faculty of Agriculture, University of Zabol, Zabol, Iran
2 Assistance Professor, Department of Landscape and Horticultural Science, Faculty of Agriculture, University of Zabol, Zabol, Iran
3 Asocciate Professor, Department of Landscape and Horticultural Science, Faculty of Agriculture, University of Zabol, Zabol, Iran
4 Instructor, Department of Landscape and Horticultural Science, Faculty of Agriculture, University of Zabol, Zabol, Iran
چکیده [English]

Today, the use of chitosan as one of plant hormones to increase plant resistance to stresses such as drought has been increased. In order to evaluate the effect of different levels of chitosan on antioxidant enzyme activities in Ajwain under drought stress, the experiment was conducted as split plot based on randomized complete block design with Three replications in research farm of Zabol University in 2014. Drought as the main factor in three levels A1) 50 mm evaporation (control), A2) 100 mm evaporation (medium stress) and A3) 150 mm evaporation (severe stress) of pan evaporation class A and different levels of chitosan spray (control, 0.1, 0.5, 1, 2 g/L) were considered as Sub factor. In this study, by increasing period between two irrigations, the activities of Catalase, Peroxidase, Ascorbate Peroxidase, Gayacolperoxidase, and polyphenoixidase enzymes were increased. There was no significant difference between control and moderate stress for most parameters but maximum activities of Antioxidant Enzymes were observed in severe stress. Using different levels of chitosan caused to improve the activity of antioxidant enzymes, so that, the interaction effect of 0.5 g/l chitosan and drought stress had increased most parameters significantly than that for  control.

کلیدواژه‌ها [English]

  • Ajwain
  • antioxdinat enzyme
  • Chitosan
  • Drought stress
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