واکنش ریزنمونه‌های مختلف یک رقم گل داوودی (Chrysanthemum morifolium) به تنظیم‌کننده‌های رشد گیاهی در محیط درون شیشه‌ای

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی سابق دکتری، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران

2 استادیار، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران

3 دانشیار، پژوهشگاه ملی مهندسی ژنتیک و زیست‌فناوری، تهران، ایران

4 دانشیار، دانشکدۀ مهندسی انرژی و فناوری‌های نوین، دانشگاه شهید بهشتی، تهران، ایران

چکیده

اهمیت اقتصادی گل داوودی در میان دیگر گونه­های زینتی سبب انجام بررسی‌های متنوعی برای بهینه­سازی کشت بافت این گیاه در سرتاسر جهان شده است. در این آزمایش، ریزنمونه­های برگ، دمبرگ، گلبرگ و TCL ساقه از داوودی رقم Reagan Elite Salmon در محیط MS حاوی ترکیب‌ها و غلظت­های متنوع NAA، BAP و TDZ کشت شدند. درصد باززایی، شمار گیاهچۀ باززاشده در هر ریزنمونه و چگونگی باززایی در هر تیمار بررسی شد. نتایج گویای برتری معنی­دار درصد باززایی شاخساره از ریزنمونۀ دمبرگ (5/80 درصد) بود؛ درحالی‌که کمینۀ باززایی از ریزنمونه­های گلبرگ به دست آمد (2/37 درصد). چگونگی باززایی شاخساره از ریزنمونه­های مختلف در تیمارهای حاوی BAP به‌تنهایی، به‌صورت مستقیم و در تیمارهای حاوی TDZ و NAA به‌صورت غیرمستقیم بود. واکنش ریزنمونه­های بررسی‌شده نسبت به تیمارهای تنظیم­کنندۀ­ رشد گیاهی از لحاظ درصد باززایی شاخساره و میانگین شمار شاخسارۀ باززایی‌شده به ازای هر ریزنمونه بسیار متفاوت بود. به‌طوری‌که از میان تیمارهای حاوی ترکیبی از NAA و هر یک از دو سایتوکینین، در T12 (5/4 میلی­گرم در لیتر BAP و 1 میلی­گرم در لیتر NAA)، دمبرگ و گلبرگ، بیشترین درصد و شمار باززایی شاخساره و ریزنمونه­های TCL ساقه، کمترین میزان باززایی را نشان دادند.

کلیدواژه‌ها


عنوان مقاله [English]

Reaction of various explants of a Chrysanthemum morifolium cultivar to plant growth regulators in vitro

نویسندگان [English]

  • Rezvanolsadat Kazeroonian 1
  • Sepideh Kalatejari 2
  • Amir Mousavi 3
  • Masoud Tohidfar 4
1 Former Ph.D. Student, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Assistant Professor, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 Associate Professor, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
4 Associate Professor, Department of Biotechnology, Faculty of Energy Engineering and New Technology, Shahid Beheshti University, Tehran, Iran
چکیده [English]

Economic importance of Chrysanthemum among the other ornamental species has resulted in performing various investigations in tissue culture optimization of this plant, worldwide. In the current experiment, leaf, petiole, petal and stem TCL explants, from Chrysanthenum morifolium cv. Reagan Elite Salmon were cultured in MS medium supplemented with different combinations and concentrations of NAA, BAP and TDZ. Regeneration percentage,number of regenerated shoots per explants and regeneration type were investigated in each treatment. Results indicated significant superiority of shoot regeneration percentage from petiole explants (80.5 percent), while minimum regeneration was achieved from the petal explants (37.2 percent). Shoot regeneration type from different explants in the treatments containing only BAP, was direct and in the treatments containing TDZ and NAA was indirect. Reactions of the explants to the PGR treatments, considering shoot regeneration percentage and average number of regenerated shoots per explants, were so different. As among the treatments consisting combinations of NAA plus each of the cytokinins, in T12 (4.5 mg/l BAP + 1 mg/l NAA), petioles and petals represented maximum shoot regeneration percentage and number, while stem TCLs showed minimum rates of regeneration.

کلیدواژه‌ها [English]

  • Callogenesis
  • explant type
  • organogenesis
  • plant growth regulators (PGRs)
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