در این پژوهش تأثیر ترکیبی از غلظتهای مختلف بنزیل‌آمینو پورین (0، 1، 5/1 و 2 میلیگرم در لیتر) و نفتالین استیک اسید (0، 25/0، 5/0 و 1 میلیگرم در لیتر) بر پیازچهزایی مستقیم و غیرمستقیم ریزنمونههای پیاز یعنی سوخ چهار بوم‌جور (اکوتیپ) موسیر (لرستان، زنجان، سنندج و اراک) در محیط کشت MS بررسی شد. نتایج نشان داد که تیمارهای مختلف هورمونی و بوم‌جور، زمان و درصد سوخک (Bulblet)زایی، شمار، طول و وزن سوخکهای باززایی‌شدۀ موسیر را تحت تأثیر قرار دادند. استفاده از بنزیل آمینو پورین (BAP) و نفتالین استیک اسید (NAA) باعث تسریع در سوخکزایی شد و در تیمار 5/1 میلیگرم در لیتر BAP همراه با 5/0 میلیگرم در لیتر NAA، بالاترین درصد سوخکزایی مستقیم (26/82%) با میانگین 13/14 عدد بیشترین شمار سوخک به دست آمد. سوخ‌های بوم‌جور اراک، درصد بالایی از سوخکزایی (61/64%) و شمار بیشتری سوخک باززایی‌شده از هر ریز نمونه (33/10) را نشان دادند. پینه (کالوس)زایی تنها در تیمار 2 میلیگرم در لیتر BAP به همراه 1 میلیگرم در لیتر NAA (05/73 درصد) و تیمار 1 میلیگرم در لیتر BAP به همراه 5/0 میلیگرم در لیتر NAA (67/23 درصد) مشاهده شد. ریشهزایی در محیط کشت بدون هورمون اکسین در زمان کمتری در مقایسه با دیگر تیمارها صورت گرفت و با درصد بالایی از ریشهزایی (92/79%) نیزهمراه بود. بنابر نتایج به‌دست‌آمده کشت سوخ‌های موسیر منطقۀ اراک در محیط کشت دارای 5/1 میلیگرم در لیتر BAP همراه با 5/0 میلیگرم در لیتر NAA برای تولید انبوه سوخکهای درون شیشهای موسیر توصیه میشود.
The effect of different concentrations of benzylaminopurine and naphthalene acetic acid on direct regeneration of explants of four Persian shallot (Allium hirtifolium) ecotypes at in vitro condition
1Ph. D. Candidate, Faculty of Agriculture, University of Tabriz, Iran
2Associate Professor, Faculty of Agriculture, University of Tabriz, Iran
3Assistant Professor, Faculty of Agriculture, University of Tabriz, Iran
چکیده [English]
In the present study, the effects of different concentrations of benzylamino purine (0, 1, 1.5 and 2 mg l-1) and naphthalene acetic acid (0, 0.25, 0.5 and 1 mg l1-) on direct and indirect bulblet rejected set by user of basal plate explants of four ecotypes of Psersian shallot (Lorestan, Zanjan, Sanandaj and Arak) on MS medium were investigated. The results showed that different hormonal treatments and ecotypes affected the time and percent of bulblet regeneration as well as the number, length and weight of regenerated bulblets. Moreover, direct and indirect regeneration of bulblet were different among the treatments. Application of BAP and NAA accelerated the bulblet regeneration of Persian shallot explants and the highest bulblet regeneration (82.26%) and maximum bulbllets number (14.13) were obtained by 1.5 mg g-1 BAP with 0.5 mg l-1 NAA. The explants of Arak ecotype showed the highest percent of bulblet regeneration (64.61%) and bulblets number (10.33) per each explant. The callus induction was only observed in 2 mg l-1 BAP+1 mg l-1 NAA (75.05%) and 1 mg l-1 BAP+0.5 mg l-1 NAA (23.67%) treatments. The rooting of bulblet in all the studied ecotypes was obtained in hormone-free medium in the shortest time (25 after culture) in comparison with other treatments that was also accompanied with the highest percent of rooting (79.917%). According to the results the culture of Arak ecotype bulbs in medium with 1.5 mg g-1 BAP and 0.5 mg l-1 NAA is advisable for invitro bulblets production of Persian shallot.
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