تأثیر تیمار پیش از برداشت پوترسین‏ بر ویژگی‏های کمی، کیفی و عمر پس از برداشت گل شاخه‌بریدۀ لیزیانتوس ((Eustoma grandiflorum cv. Miarichi Grand white

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 استادیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

چکیده

این آزمایش باهدف بررسی تأثیر محلول‏پاشی برگی پوترسین در مرحلۀ پیش از برداشت بر کیفیت رویشی و ویژگی‏های پس از برداشت گل شاخه‌بریدۀ لیزیانتوس رقم میاریچی گرند وایت انجام شد. گیاهان در گلدان‏های محتوای خاک زراعی، ماسه و خاک‌برگ پوسیده با نسبت 1:1:1 حجمی کشت شدند. تیمار‏ها در مرحلۀ پیش از برداشت شامل پوترسین با چهار غلظت 0 (شاهد)، 5/0، 1 و 2 میلی‌مولار بودند که در مرحلۀ انگیزش غنچه انجام شد. صفات مربوط به کیفیت رویشی شامل طول و قطر غنچه، ارتفاع ساقۀ گل‏دهنده و وزن تر و خشک آن، سطح برگ و محتوای سبزینه (کلروفیل) بودند. نتایج نشان داد، تیمار پوترسین منجر به افزایش بسیاری از ویژگی‏ها مانند طول و قطر غنچه، ارتفاع ساقۀ گل‏دهنده و وزن تر و خشک آن، سطح برگ و محتوای سبزینۀ برگ نسبت به تیمار شاهد شد. همچنین در بین غلظت‏ها پوترسین 2 میلی‌مولار بیشترین تأثیر را بر عامل‏های اندازه‏گیری‌شده داشتند. به‌منظور بررسی تأثیر تیمارهای پیش از برداشت پوترسین بر ویژگی‏های پس از برداشت گل شاخه‌بریدۀ لیزیانتوس، گل‏ها در مرحله‏ای که دو غنچۀ آن به‌طور کامل باز شدند، برداشت شدند. نتایج نشان داد، تیمارهای پیش از برداشت منجر به افزایش عمر گلجایی، حفظ بهتر وزن تر نسبی و جذب آب نسبی نسبت به تیمار شاهد شدند. همچنین کاهش میزان نشت یونی، تجمع کمتر مالون‏دی‏آلدئید، فعالیت کمتر آنزیم لیپوکسیژناز، تجمع کمتر H2O2 و بهبود آنزیم‏های پاداکسندۀ (آنتی‌اکسیدان) کاتالاز و آسکوربات پراکسیداز در غلظت 2 میلی‌مولار پوترسین نسبت به تیمار شاهد به دست آمد.

کلیدواژه‌ها


عنوان مقاله [English]

Impact of preharvest putrescine treatment on quantitative, qualitative traits and postharvest vase life of Lisianthus (Eustoma grandiflorum cv. Miarichi Grand white) cut flowers

نویسندگان [English]

  • Davoud Ataii 1
  • Roohangiz Naderi 2
  • Azizollah Khandan-Mirkohi 3
1 Former M.Sc. Student, Professor, Assistant Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Assistant Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

This experiment was conducted to evaluate the effect of foliar application of putrescine on the pre-harvest stage on vegetative quality and postharvest attribtes of Lisianthus ‘Miarichi Grand white’ cut flowers. Plants were planted in the pots containing soil, sand and peat with a volume ratio of 1: 1: 1. The pre-harvest putrescine treatments by 0 (distilled water), 0.5, 1, and 2 mM were applied as foliar spray at the stage of bud initiation. Vegetative quality attributes such as number of flower bud, flower bud length and diameter, flower stem length, flower stem fresh and dry weight, leaf area and chlorophyll content were measured. Results showed that application of putrescine increased number of flower bud, flower bud length and diameter, flower stem length, flower stem fresh and dry weight, leaf area and chlorophyll content compared to the control. Also, putrescine at 2 mM was the most effective treatments in this study. To investigate the effects of preharvest putrescine on postharvest characteristics of Lisianthus cut flowers, the flowers were harvested when two flowers were fully opened. The results showed that pre-harvest treatment led to increase vase life, relative fresh weight and relative water uptake compared to the control. Also, the decrease in ionic leakage, less accumulation of malondialdehyde, reduced enzyme activity lipoxygenase, less accumulation of H2O2 and increase of antioxidant enzymes catalase and ascorbate peroxidase in the concentration of 2 mM putrescine were observed.

کلیدواژه‌ها [English]

  • Antioxidant Enzymes
  • lipoxygenase
  • Malondialdehyde
  • vase life
  • vegetative quality
  • water uptake
  1. Abdel Aziz Nahed, G., Taha Lobna, S. & Ibrahim Soad, M. (2009). Some studies on the effect of putrescine, ascorbic acid and thiamine on growth, flowering and some chemical constituents of gladiolus plants at Nubaria. Ozean Journal of Applied Sciences, 2(2).
  2. Aebi, H. (1984). Catalase in vitro. Methods Enzymology, 105, 121-126.
  3. Alaey, M. (2011). Study the effect of salicylic acid in pre- and postharvest treatment on physio-chemical characteristics and vase-life of Rose hybrid L. Black Magic. Ph.D. Thesis. Faculty of Agriculture Tehran University, Iran. (in Farsi)
  4. Alexieva, V., Sergiev, I., Mapelli, S. & Karanov, E. (2001). The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant Cell and Environment, 24, 1337-1344.
  5. Arnon, A. N. (1967). Method of extraction of chlorophyll in the plants. Agronomy Journal, 23, 112-121.
  6. Arrom, L. & Munné-Bosch, S. (2010). Tocopherol composition in flower organs of Lilium and its variations during natural and artificial senescence. Plant Science,179, 289-295.
  7. Axelroad, B., Cheesebrough, T. M. & Laasko, S. (1981). Lipoxigenase from soybeans. Methods Enzymology, 71, 441-451.
  8. Azad, A. K., Ishikawa, T., Ishikawa, T., Sawa, Y. & Shibata, H. (2008). Intracellular energy depletion triggers programmed cell death during petal senescence in tulip. Journal of Experimental Botany, 59, 2085-2095.
  9. Bagni, N., Tassoni, A. & Teixeira daSilva, J. A. (2006). In: J.A. Teixeira daSilva (Ed), the role of polyamines in relation to flower senescence. Floriculture, ornamental and plant biotechnology. (pp. 88-95)Springer Science.
  10. Bailly, C., Corbineau, F. & van Doorn, W. G. (2001). Free radical scavenging and senescence in Iris tepals. Plant Physiology and Biochemistry,39, 649-656.
  11. Bais, H. P. & Ravishankar, G. A. (2002). Role of polyamines in the ontogeny of plants and their biotechnological applications. Plant Cell Tissue Organ Culture, 69, 1-34.
  12. Bouchereau, A., Aziz, A., Larher, F. & Martin-tanguy, J. (1999). Polyamines and environmental challenges: recent development. Plant Science, 140, 103-125.
  13. Chakrabarty, D., Chatterjee, J. & Datta, S. K. (2007). Oxidative stress and antioxidant activity as the basis of senescence in chrysanthemum florets. Plant Growth Regulation, 53, 107-115.
  14. Fukuchi-Mizutani, M., Ishiguro, K., Nakayama, T., Utsunomiya, Y., Tanaka, Y., Kusumi, T. & Ueda, T. (2000). Molecular and functional characterization of a rose lipoxygenase cDNA related to flower senescence. Plant Science, 160, 129-137.
  15. Ghahsare, M. & Kafi, M. (2012). Floriculture. Pearson Prentice Hall. (in Farsi)
  16. Hong, Y., Wang, T. W., Katalin, A., Hudak, F. S., Carol, D., Froese, J. & Thompson, E. (2000). An ethylene induced cDNA encoding a lipase expressed at the onset of senescence. PNAS, 97, 871722.
  17. Hossain, Z., Mandal, A. K. A., Datta, S. K. & Biswas, A. K. (2006). Decline in ascorbate peroxidase activity a prerequisite factor for tepal senescence in Gladiolus. Journal of Plant Physiology, 163, 186-194.
  18. Hosseini-Farahi, M., Khalighi, A., Kholdbarin, Mashhadi, B., Akbar-boojar, M. & Eshghi, S. (2013). Morphological responses and vase life of Rosa hybrida cv. Dolcvita to polyamines spray in hydroponic system. World Applied Sciences Journal, 21(11), 1681-1686.
  19. Jiang, Y. M. & Chen, F. (1995) A study on polyamine change and browning of fruit during cold storage of litchi fruit. Postharvest Biology and Technology, 5, 245-250.
  20. Joyce, D. C. & Jones, P. N. (1992). Water balance of the foliage of cut Geraldton waxflower. Postharvest Biology and Technology, 2, 31-39.
  21. Lee, M. M., Lee, S. H. & Park, K. Y. (1997). Effects of spermine on ethylene biosynthesis in cut carnation (Dianthus caryophyllus L.) flowers during senescence. Journal of plant physiology, 151(1), 68-73.
  22. Liu, J. H., Kitashiba, H., Wang, J., Ban, Y. & Moriguchi, T. (2007). Polyamines and their ability to provide environmental stress tolerance to plants. Plant Biotechnology, 24(1), 117-126.
  23. Mahgoub, M. H., Abdel Aziz Nahed, G. & Mazhar, M. A. (2011). Response of Dahila pinnata L. plant to floiar spray white putrescine and thiamine on growth, flowering and photosynthetic pigments. American-Eurasian Journal of Agricultural & Environmental Sciences, 10(5), 769-775.
  24. Mahros, K. M., El-Saady, M. B., Mahgoub, M. H., Afaf, M. H. & El-Sayed, M. I. (2011). Effect of putrescine and uniconazole treatments on flower characters and photosynthetic pigments of Chrysanthemum indicum L. plant. Journal of American Science, 7, 399-408.
  25. Majidiyan, N. (2013). Study some aspects of flower Senescence in Asiatic hybrid lily Seb Dassel. Ph.D. thesis. Faculty of Agriculture Tehran University, Iran. (in Farsi)
  26. Panavas, T., Walker, E. L. & Rubinstein, B. (1998). Possible involvement of abscisic acid in senescence of daylily petals. Journal of Experimental Botany, 49(329), 1987-1997.
  27. Motozu, T. & Makihara, T. (2001). Selection of useful cultivars in Eustoma grandiflorum with flower longevity as an additional selecting factor. Journal of the Japanese Society for Horticultural Science, 9, 23-28.
  28. Pandey, S., Ranade, S. A., Nagar, P. K. & Kumar, N. (2000). Role of polyamines and ethylene as modulators of plant senescence. Journal of Biosciences, 25(3), 291-299.
  29. Ranieri, A., Castagna, J., Pacini, B., Baldan, A. & Mensuali Sodi, G. F. (2003). Soldatini early production and scavenging of hydrogen peroxide in the apoplast of sunflowers plants exposed to ozone. Journal of Experimental Botany, 54, 2529-2540.
  30. Rogers, H. J. (2012). Is there an important role for reactive oxygen species and redox regulation during floral senescence?. Plant, Cell & Environment, 35(2), 217-233.
  31. Rubinowska, K. & Miachałek, W. (2009). Influence of putrescine on leaf senescence of Helianthus annuus L. potted plants. Horticulture and Landscape Architecture, 30, 57-65.
  32. Rubinowska, K., Pogroszewska, E. & Michałek, W. (2012). The effect of polyamines on physiological parameters of post-harvest quality of cut stems of Rosa ‘Red Berlin’. Acta Scientiarum Polonorum Hortorum Cultus, 11(6).
  33. Shahri, W. & Tahir, I. (2011). Flower senescence-strategies and some associated events. Botanical Review, 77, 152-184.
  34. Shimizu, H. & Ichimura, K. (2010). Postharvest physiology and technology of cut Eustoma flowers. Journal of the Japanese Society for Horticultural Science, 79(3), 227-238.
  35. Sivaprakasam, G., Singh, V. P. & Arora, A. (2009). Physiological and molecular analysis of effect of spermine on senescing petals of gladiolus. Indian Journal of Plant Physiology, 14(4), 384-391.
  36. Soleimany-Fard, E., Hemmati, K. & Khalighi, A. (2014(. Impact of pre- and post-harvest putrescine applications on water relations and vase life of cut alstroemeria flowers. Advances in Environmental Biology, 8(12), 158-165.
  37. Stewart, R. C. & Beweley, J. D. (1980). Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiology, 25, 136-1.
  38. van Doorn, W. G. & Woltering, E. J. (2008). Physiology and molecular biology of petal senescence. Journal of Experimental Botany, 59(3), 453-480.
  39. Yamane, K., Kawabata, S. & Fujishige, N. (1999). Changes in activities of superoxide dismutase, catalase and peroxidase during senescence of Gladiolus florets. Journal of the Japanese Society for Horticultural Science,68, 798-802.
  40. Yang, C. W., He, S. G., Jiang, Y. M. & Yi, S. (2000). Effects of polyamines on biochemical and physiological changes and vase life of cut rose (Rosa chinensis Jacq. cv. Bellamie) flowers during senescence. Journal of Tropical and Subtropical Botany, 8(2), 104-108.
  41. Youssef, A. A., Mahgoub, M. H. & Talaat, I. M. (2004). Physiological and biochemical aspects of Matthiola incana plants under the effect of Putrescine and Kinetin treatments. Egypt journal applied science, 19(9B) 492-510.
  42. Zhang, Y., Guo, W., Chen S., Han, L. & Li, Z. (2007). The role of N-lauroylethanolamine in the regulation of senescence of cut carnations (Dianthus caryophyllus). Journal of Plant Physiology, 164, 993-1.