بررسی شاخص‌های اکسیداتیو در سه رقم انگور (Vitis vinifera L.) در شرایط تنش خشکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 استادیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

چکیده

به‌منظور بررسی واکنش­های فیزیولوژیکی و بیوشیمیایی برخی رقم‌های انگور تحت تنش خشکی، آزمایشی به‌صورت فاکتوریل در قالب طرح بلوک­های کامل تصادفی با سه رقم انگور غیرپیوندی بی‌دانۀ سفید، چفته و یاقوتی و چهار تیمار خشکی در حد 3/0- (شاهد)، 6/0- ( ملایم)، 1- (متوسط) و 5/1- (شدید) مگاپاسکال پتانسیل آب خاک، در سه تکرار در سال 1392 به اجرا درآمد. در این آزمایش گیاهان دوساله انگور در گلدان­های 20 لیتری (قطر گلدان 28 سانتی‌متر و ارتفاع گلدان 60 سانتی‌متر) محتوای خاک لومی- شنی در فضای آزاد (مزرعه) کاشته شدند. نتایج این پژوهش نشان داد که در رقم بی­دانه با افزایش سطوح تنش خشکی، محتوای نسبی آب برگ (RWC)، میزان سبزینۀ (کلروفیل) a و b کاهش یافت ولی میزان نشت یونی، تجمع مالون­دی­آلدئید(MDA)، فعالیت آنزیم لیپوکسیژناز (LOX) که مسئول پراکسیداسیون اسیدهای چرب غشا است به همراه میزان پراکسید هیدروژن (H2O2) افزایش یافتند. همچنین در فعالیت آنزیم­های پاداکسندگی (آنتی‌اکسیدانی) شامل کاتالاز (CAT)، آسکوربات پراکسیداز (APX)، گایکول پرکسیداز و ظرفیت پاداکسندگی کل (DPPH) تغییر معنی‌داری مشاهده نشد که نشان­دهندۀ این واقعیت است که رقم بی‌دانۀ سفید تحت تنش خشکی کمترین میزان انسجام غشایی را دارد. رقم چفته نیز بالاترین میزان RWC و میزان سبزینۀ a و b در مقایسه با رقم‌های بی­دانۀ ­سفید و یاقوتی را داشت. برخلاف رقم بی‌دانۀ سفید، رقم‌های چفته و یاقوتی در سطوح تنش خشکی توانایی بالاتری در حفظ انسجام غشایی داشتند و میزان بالاتری از فعالیت آنزیم­های پاداکسندۀ CAT، APX،GPX ، به همراه تجمع پایین H2O2 را داشتند. با توجه به یافته‌های این پژوهش می‌توان گفت که رقم چفته و در مراحل بعد رقم یاقوتی پتانسیل بالاتری برای رویارویی با شرایط کم‌آبی در مقایسه با رقم بی‌دانۀ سفید دارند.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of oxidative parameters in three grapevine cultivars under drought stress

نویسندگان [English]

  • Reza Soukhtesaraee 1
  • Ali Ebadi 2
  • Seyed Alireza Salami 3
  • Hossein Lesani 2
1 Former M. Sc. Student, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Assistant Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

For evaluation of physiological and biochemical response of some grapevine cultivars to drought stress, an experiment was carried out in 2014 as a factorial arranged in a randomized complete block design with three replications. Three cultivars i. e. “Bidane Sefid”, “Chafte” and “Yaghooti” and four drought stress levels, including -0.3, -0.6, -1 and -1.5 MP soil water potential were employed. In this experiment, two years old grapes were cultured in pots with 28 cm in diameter and 60 cm in height, containing sandy-loam soil in field conditions. Results showed that in “Bidane sefid”, under drought stress (-0.3 to -1.5 MP), RWC, chlorophyll a and b content, and antioxidant enzymes CAT, APX, GPX activity along with DPPH scavenging capacity were declined, but electrolyte leakage, MDA content, LOX enzyme activity (responsible for membrane unsaturated fatty acid peroxidation), along with H2O2 accumulation were increased. Results indicated that ‘Bidane Sefid’ under drought stress had higher oxidative damage, led to lower membrane integrity. In comparison with “Bidane sefid”, “Chafte” and “Yaghooti” under drought stress, due to higher antioxidant enzymes (CAT, APX, GPX) with higher DPPH scavenging capacity, exhibited lower oxidative damage, led to higher membrane integrity. According to our results, it can be concluded that “Chafte” followed by “Yaghooti” had higher potential to tolerate drought stress compared with “Bidane sefid”.

کلیدواژه‌ها [English]

  • Antioxidant Enzymes
  • Drought stress
  • Grapevine
  • Membrane integrity
  • oxidative stress
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