تأثیر کاربرد برگی پلی‌آمین‌ها بر ویژگی‌های رشدی، عمر گل‌جایی و میزان تنظیم‌کننده‌های رشد درونی گل رز رقم دولس ویتا

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، باشگاه پژوهشگران جوان و نخبگان، واحد یاسوج دانشگاه آزاد اسلامی- واحد یاسوج، یاسوج، ایران

2 استادیار، گروه علوم باغبانی، واحد شیراز، دانشگاه آزاد اسلامی- واحد شیراز، شیراز، ایران

چکیده

 بهبود رشد و گلدهی با کاربرد پلی­آمین­ها در گیاهان مختلفی گزارش شده ­است. بنابراین در این پژوهش کاربرد برگی پلی­آمین­های برون­زاد بر ویژگی‌های کمی، عمر گل­جایی (Vase life)، میزان تنظیم‌کننده­های رشد درونی و پلی­آمین­های درونی گل رز بررسی شد. بدین منظور، آزمایشی به‌صورت طرح بلوک­های کامل تصادفی با ده تیمار، سه تکرار و دو بوته در هر تکرار در یک گلخانة آبکشتی (هیدروپونیک) تجاری در شهر یاسوج انجام گرفت. تیمارها شامل محلول­پاشی بوته­ها با پوتریسین در غلظت­های 1، 2 و 3 میلی­مولار، اسپرمیدین در غلظت­های 5/0، 1و 5/1 میلی­مولار و اسپرمین در غلظت­های 1، 2و 4 میلی­مولار و شاهد (آب مقطر) بود. نتایج نشان داد، بیشترین و کمترین میزان ارتفاع و وزن شاخه به ترتیب در تیمارهای اسپرمیدین 5/1 میلی­مولار و شاهد به دست آمد. کاربرد پلی­آمین­ها باعث افزایش عمر گل­جایی شد به­طوری­که بیشترین عمر گل­جایی به میزان شانزده روز در گیاهان تیمارشده با اسپرمین 1 میلی­مولار مشاهده شد. بیشترین میزان غلظت ایندول­استیک­­اسید، اسیدجیبرلیک و زاتین در برگ گیاهان محلول­پاشی شده با غلظت 4میلی­مولار اسپرمین مشاهده شد. همچنین بیشترین میزان اسیدآبسایزیک در برگ گیاهان محلول­پاشی شده با اسپرمیدین 5/1 میلی­مولار به دست آمد. کاربرد پلی­آمین­های برون­زاد باعث افزایش میزان پوتریسین و اسپرمیدین درونی برگ شد. نتایج کلی این پژوهش نشان داد که کاربرد اسپرمیدین در غلظت 5/1 میلی‌مولار و اسپرمین 4 میلی‌مولار برون­زاد به دلیل افزایش غلظت مواد تنظیم‌کنندۀ رشد و پلی‌آمین‌های درونی، باعث بهبود ویژگی‌های رویشی و افزایش عمر گل­جایی گل رز رقم دولس ویتا شد. 

کلیدواژه‌ها


عنوان مقاله [English]

Effect of foliar application of polyamines on growth properties, vase life and endogenous plant growth regulators contents of cut rose flower (Rosa hybrida cv. Dolcvita)

نویسندگان [English]

  • Mehdi Hosseini Farahi 1
  • Masoud Zadehbagheri 2
1 Assistant Professor, Young Researcher and Elite Club, Yasooj Branch, Islamic Azad University, Yasooj, Iran
2 Assistant Professor, Department of Horticulture Science, Shiraz Branch, Islamic Azad University, Shiraz, Iran
چکیده [English]

Improvement of growth and flowering with polyamines application has been reported in different plants. So, in this study application of exogenous polyamines on quantitative characteristics, vase life and endogenous hormones and polyamines contents in rose flower was investigated. For this purpose, an experiment was conducted as randomized complete block design (RCBD) with ten treatments, three replications and two observations in each replication in a hydroponic greenhouse in Yasouj city. Treatments were including foliar application of putricine (Put) at 1, 2 and 3 mM, spermidine (Spd) at 0.5, 1 and 1.5 mM, spermin (Spm) at 1, 2 and 4 mm and control. The highest and the lowest fresh weight and flower height were obtained in the plants treated with Spd 1.5 mM and control, respectively. Foliar application of polyamines increased the vase life, so that maximum vase life (16 days) was observed in treated flowers with Spm 1 mM as compared to the other treatments. The highest content of IAA, GA3 and zeatin obtained in treated flowers with Spm 4 mM. The highest ABA content was found in treated flowers with Spd 1.5 mM. The use of exogenous polyamine increased the internal Put and Spd contents. So, the final results of this study revealed that exogenous application of 1.5 mM Spd and 4 mM Spm due to increasing of internal hormones and PAS, caused to increase of vegetative properties and vase life of rose cv. Dolcvita.

کلیدواژه‌ها [English]

  • IAA
  • GA3
  • Put
  • Spd
  • Spm and Zeatin
  1.  

    1. Abdel Aziz Nahed, G., Taha Lobna, S. & Ibrahim Soad, M. M. (2009). Some studies on the effect of putrescine, ascorbic acid and thiamine on growth, flowering and some chemical constituents of gladiolus Plants at Nubaria. Ozean Journal of Applied Sciences, 2(2), 169-179.
    2. AbdEl-Wahed, M. S. A. (2006). Exogenous and endogenous polyamines relation to growth, α–cellulose precipitation in fibres productivity of cotton plant. World Journal of Agricultural Science, 2(2), 139-148.
    3. AbdEl-Wahed, M. S. A. & Gamal El Din, K. M. (2004). Stimulation effect of spermidine and stigmasterol on growth, flowering, biochemical constituents and essential oil of Chamomile plants (Chamomilla recutita L. Rausch). Bulgarian Journal Plant Physiology, 30(3-4), 48-60. 
    4. Alcázar, R., Marco, F., Cuevas, J.C., Patron, M., Ferrando A., Carrasco, P., Tiburcio, A. F. & Altabella, T. (2006). Involvement of polyamines in plant response to abiotic stress. Biotechnology letters, 28, 1867-1876.
    5. Altman, A. (1989). Polyamines and plant hormones. In: Bachrach U, Heimer YM, (eds.), The physiology of polyamines, Vol. 31. Boca Raton, FL: CRC Press Inc, 121-145.
    6. Anjum, M. A. (2011). Effect of exogenously applied spermidine on growth and physiology of citrus rootstock Troyer citrange under saline conditions. Turkish Journal Agriculture Forestry, 35, 43-53.
    7. Bais, H. P & Ravishankar, G. A. (2002). Role of polyamines in the ontogeny of plants and their biotechnological applications. Plant Cell, Tissue and Organ Culture, 69, 1-34.
    8. Benavides, M. D., Gallegoo, S. M., Comba, M. E. & Tomaro, M. L. (2000). Relationship between polyamines and paraquat toxicity in sunflower leaf discs. Plant Growth Regualators, 31(3), 215-224.
    9. Borrell, A., Carbonell, L., Farras, R., Puig-Parellads, P. & Tiburcio, A. F. (1997). Polyamines inhibit lipid peroxidation in senescing Oat leaves. Physiology Plant, 99, 385-390.
    10. Bouchereau, A., Aziz, A., Larher, F. & Martin-Tanguy, J. (1999). Polyamines and environmental challenges: Recent development. Plant Science, 140,103-125.
    11. Costa, M. L., Civello, P. M., Chaves, R. & Martinez, C. A. (2002). Characterization of Mg-dechelase activity obtained from Fragaria ananasa fruit. Plant Physiology Biochemestry, 10, 111-118.
    12. Dastyaran, M. & Hosseini Farahi, M. (2015). Effects of humic acid and puterscine on vegetative properties and vase life of rose in soilless culture system. Journal of Science and Technology of Greenhouse Culture, 5(20), 241-250.
    13. Dantuluri, V. S. R., Misra, R. L. & Singh, V. P. (2008). Effect of polyamines on post harvest life of Gladiolus spikes. Journal of Ornamental Horticulture, 11(1), 66-68.
    14. Davies, W. J., Mansfield, T. A. & Hetherington, A. M. (1990). Sensing of soil water status and the regulation of plant growth and development. Plant Cell Environmental, 13, 709-720.
    15. El-Sayed, I. M. (2009). Physiological and biological studies on chrysanthemum plant. MSc. Agricultural Science (Ornamental Horticulture), Faculty of Agricultural, Cario Univesity, Egypt.
    16. Ergun, N., Topcuoglu, S. F. & Yildiz, A. (2002). Auxin (Indol-3-acetic acid), gibberellic aicd (GA3), abscisic acid (ABA) and cytokinin (Zeatin) production by some species of mosses and lichens. Turkish Journal of Botany, 26, 13-19.
    17. Esna-Ashari, M. & Zokaee Khosroshahi, M. R. (2008). Polyamines & Horticultural Science. Bu-ali Sina University Press No 293. 1St Ed. 188 p.
    18. Fuhrer, J., Kaur–Sawhney, R. Shin, L. M. & Galston, A. W. (1982). Effect of exogenous 1, 3 Diaminopropane and spermidine on senescence of Oat leaves. II Inhibition of ethylene biosynthesis and possible mode of action. Plant Physiology, 70, 1597-1600.
    19. Galston, A. W. & Kaur-Sawhney, R. (1990). Polyamines in plant physiology. Plant Physiology, 94, 406-410.
    20. Hosseini Farahi, M., Eshghi, S., Kavoosi, B., Amiri Fahliani, R & Dastyaran, M. (2013). Effects of spermidine and calcium sulfate on quantitative and qualitative traits and vase life of rose (Rosa hybrida cv. Dolcvita) grown in hydroponic system. Journal of Science and Technology of Greenhouse Culture, 4(14), 15-26.
    21. Hussein, M.M., Nadia, H., El-Geready, M. & Elesuki, M. (2006). Role of puterscine in resistance to salinity of pea plants (Pisum sativm). Journal of Applied Science Research, 2(9), 598-604.
    22. Kuznetsov, V. K. & Shevyakovaf, N. I. (2007). Polyamiens and stress tolerance of plants. Plant Stress, 1(1), 50-71.
    23. Lobna, S. T. & Rawiaid, A. E. (2011). Stimulation effect of some bioregulators on flowering, chemical constituents, essential oil and phytohormones of tuberose (Polianthes tuberos L.). New York Science Journal, 4(5), 16-21.
    24. Leea, M. M., Leea, S. H. & Parkb, K. Y. (1997). Effects of spermine on ethylene biosynthesis in cut Carnation (Dianthus caryophyllus L.) flowers during senescence. Journal of Plant Physiology, 151(1), 68-73.
    25. Mahgoub, M. H., Abd El Aziz, N. G. & Mazhar, M. A. (2011). Response of Dahlia pinnata L. plant to foliar spray with putrescine and thiamine on growth, flowering and photosynthetic pigments. American-Eurasian Journal Agriculture and Environmental Science, 10(5), 769-775.
    26. Mahgoub, M. H., El-Ghorab, A. H. & Bekheta, M. H. (2006). Effect of some bioregulators endogenous phytohormones, chemical composition, essential oil and its antioxidant activity carnation (Dianthus caryophyllus L.). Journal of Agriculture Science. Mansoura University, 31(7), 4229-4245.
    27. Mahros, K. M., El-Saady, M. B., Mahgoub, M. H., Afaf, M. H. & El-Sayed, M. I. (2011). Effect of putrescine and uniconazole treatments on flower characters and photosynthetic pigments of Chrysanthemum indicum L. Plant. Journal of American Science, 7(3), 399-408.
    28. Martin-Tanguy, J. (2001). Metabolism and function of polyamines in plants. Recent development (new approaches). Plant Growth Regulators, 34(14), 135-148.
    29. Narcin, D. (1995). Stress and polyamine metabolism. Bulgarian Journal Plant Physiology, 21(2-3), 3-14.
    30. Nemeth, M., Janda, T., Horvath, E., Paldi, E. & Szalai, G. (2002). Exogenous salicylic acid increases polyamine content but may decrease drought tolerance in maize. Plant Science, 162, 569-574.
    31. Palavan-Ünsal, N. (1995). Stress and polyamine metabolism. Bulgarian Journal of Plant Physiology, 21, 3-14.
    32. San-Francisco, S., Houdusse, F., Zamarreño, A.M., Garnica, M., Casanova, E. & García-Mina, J. M. (2005). Effects of IAA and IAA precursors on the development, mineral nutrition, IAA content and free polyamine content of pepper plants cultivated in hydroponic conditions. Scientia Horticulture, 106, 38-52.
    33. Silveira, V., Santa-Catarina, C., Tun, N. N., Scherer, G. F. E., Handro, W., Guerra, M. P. & Floh, E. I. S. (2006). Polyamine effects on the endogenous polyamine contents, nitric oxide release, growth and differentiation of embryogenic suspension cultures of Araucaria angustifolia (Bert.) O. Ktze. Plant Science, 171, 91-98.
    34. Sood, S. & Nagar, P. K. (2008). Post-harvest alterations in polyamines and ethylene in two diverse rose species. Acta Physiology Plant, 30, 243-248.
    35. Sood, S. & Nagar, P. K. (2003). The effect of polyamines on leaf senescence in two diverse rose species. Plant Growth Regulation, 39, 155-160.
    36. Steiner, N., Santa-Catarina, C., Silveira, V., Floh, E. I. S. & Guerra, M. P. (2007). Polyamine effects on growth and endogenous hormones levels in Araucaria angustifolia embryogenic cultures. Plant Cell, Tissue and Organ Culture, 89, 55-62.
    37. Unyayar, S., Topcuoglu, S. F. & Unyayar, A. (1996). A modified method for extraction and identification of indole-acetic acid (IAA), gibberellic acid (GA3), abscisic acid (ABA) and Zeatin produceby phanerochaete chrysosporium ME446. Bulgarian Journal Plant Physiology, 22(3-4), 105-110.
    38. Talaat, I, M., Bekhea, M. A. & Mahgoub, M. H. (2005). Physiological response of periwinkle plants (Catharanthus roseus L.) to tryptophan and putrescine. International Journal of Agriculture and Biology, 2, 210-213.
    39. Tassoni, A., Accettulli, P. & Bagni, N. (2006). Exogenous spermidine delays senescence of Dianthus caryophyllus flowers. Plant Biosystems, 140, 107-114.
    40. Thomas, T. & Thomas, T. J. (2001). Polyamines in cell growth and cell death: molecular mechanisms and therapeutic applications. Cellular and Molecular Life Sciences, 58, 224-258.
    41. Youssef, A. A., Mahgoub, M. H. & Talaat, M. I. (2004). Physiological and biochemical aspects of Matthiola incana L. plants under the effect of putrescine and kinetin treatments. Egypt Journal of Applied Science, 19(9B), 492-510.