اثر اسید سالیسیلیک و پوتریسین بر برخی شاخص‌های فیزیولوژیکی و اسانس گیاه دارویی نعناع فلفلی (Mentha piperita L.)

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، گروه علوم باغبانی، دانشکدۀ کشاورزی، دانشگاه تبریز

2 دانشیار، گروه علوم باغبانی، دانشکدۀ کشاورزی، دانشگاه تبریز

3 استادیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه تبریز

چکیده

نعناع فلفلی با نام علمیL.  Mentha piperita از خانوادۀ Lamiaceae از جمله گیاهان دارویی و معطری است که اسانس آن در صنایع مختلف داروسازی استفاده می­شود. به‌منظور ارزیابی برخی شاخص­های فیزیولوژیکی و اسانس نعناع فلفلی آزمایشی به‌صورت فاکتوریل با استفاده از تیمارهای اسید سالیسیلیک (0، 1/0، 1 میلی‌مولار) و پوتریسین (0، 1/0، 1 میلی­مولار) در قالب طرح پایه بلوک‌های کامل تصادفی با سه تکرار اجرا شد. همۀ صفات در دو چین ارزیابی شدند. نتایج به‌دست‌آمده از این پژوهش نشان داد که میانگین همۀ صفات (به‌جز نسبت سبزینۀ (کلروفیل) a/b، کاروتنوئید، آنتوسیانین و مواد جامد محلول) در چین اول به‌طور معنی‌داری بیشتر از چین دوم بودند. کاربرد غلظت­های مختلف اسید سالیسیلیک منجر به کاهش میزان سبزینۀ کل و a، کارتنوئید و آنتوسیانین شد. همچنین تیمارهای 1/0 و 1 میلی­مولار اسید سالیسیلیک درصد اسانس شاخسارۀ نعناع فلفلی را در مقایسه با مصرف نکردن آن، 02/16و 91/32 درصد و نیز درصد اسانس برگ گیاه را نسبت به شاهد، 54/2 و 66/29 درصد افزایش داد. با افزایش مقادیر پوتریسین، عملکرد تر و خشک در واحد سطح، سبزینۀa ، سبزینۀ کل، کاروتنوئید و درصد اسانس برگ افزایش یافت. با به‌کارگیری 1 میلی­مولار پوتریسین میزان آنتوسیانین و درصد اسانس برگ در مقایسه با شاهد به ترتیب 91/33 و 36/21 درصد افزایش یافت. افزون بر آن اثر متقابل دو عامل مورد بررسی روی عملکرد تر در واحد سطح، سبزینۀ a وb، سبزینۀ کل، کاروتنوئید، آنتوسیانین، مواد جامد محلول و اثر متقابل سه عامل بر عملکرد خشک در واحد سطح، کاروتنوئید و مواد جامد محلول معنی­دار بودند.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of salicylic acid and putrescine on some physiological characters and essential oil of peppermint (Mentha piperita L.)

نویسندگان [English]

  • Somayeh Najjarzadeh 1
  • Jaber Panahandeh 2
  • Saeedeh Alizadeh Salteh 3
  • Fariborz Zaare-Nahandi 2
1 M.Sc. Student, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2 Associate Professor, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
3 Assistant Professor, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
چکیده [English]

Peppermint (Mentha piperita L.) is a medicinal and aromatic plant belonging to Lamiaceae family that its essential oil is used in differernt pharmaceutical industries. In order to study the effect salicylic acid and putrescine on some physiological characters and essential oil of peppermint, an experiment was conducted as a factorial using treatments of salicylic acid (0, 0.1, 1 mM) and putrescine (0, 0.1, 1 mM) in randomized complete block design with three replications. All traits were evaluated in two harvests. Results showed that all traits (except the ratio of chlorophyll a/b, carotenoid, anthocyanin and soluble solids) in the first harvest were significantly higher than the second harvest. Application of different concentrations of salicylic acid led to decrease the amount of chlorophyll a, total chlorophyll, carotenoid and anthocyanin. Also the treatments of 0.1 and 1 mM salicylic acid increased shoot essential oil perecentage in comparison to no consumption 16.45% and 32.91% and leaf oil perecentage in comparison to control 2.54% and 29.66%, respectively. With increasing the putrescine, yield per unit area, chlorophyll a, total chlorophyll, carotenoid and leaf essential oil perecentage were increased. Applying of 1mM putrescine increased the amount of anthocyanin and leaf essential oil perecentage in comparison to control 33.91% and 21.36%, respectively. In addition to, the interaction effect of two studied factors on fresh yield per unit area, chlorophyll a and b, total chlorophyll, carotenoid, anthocyanin, soluble solids and interaction effect of three factors on dry yield per unit, careatotal and soluble solids were significant.

کلیدواژه‌ها [English]

  • Essential oil
  • growth regulators
  • Medicinal plant
  • polyamin
  • Salicylic Acid
  • vegetative traits
  1. Abdel Aziz Nahed, G., Taha Lobna, S. & Ibrahim Soad, M. M. (2009). Some Studies on the Effect of Putrescine, Ascorbic Acid and Thiamine on Growth, Flowering and Some Chemical Constituents of Gladiolus Plants at Nubaria. Ozean Journal of Applied Sciences, 2(2), 169-179.
  2. Ali, R M., Abbas, H. M. & Kamal R. K. (2007). The effect of treatment with polyamines on dry matter, oil and flavonoid contents in salinity stressed chamomile and sweet marjoram. Plant Soil Environment, 53(12), 529-543.
  3. Arias, M., Carbonell, J. & Agusti, M. (2005). Endogenous free polyamines and their role in fruit set of low and high parthenocarpic ability citrus cultivars. Plant Physiology, 126(8), 845-853.
  4. Ayad, H.S., Reda, F. & Abdalla. M. S. A. (2010). Effect of putrescine and zinc on vegetative growth, photosynthetic pigments lipid peroxidation and essential oil content of geranium (Pelargonium graveolens L.). World Journal Agriculture Science, 6(5), 601-608.
  5. Behnam. S., Farzaneh. M., Ahmadzadeh. M. & Tehrani. A. (2006). Composition and antifungal activity of essential oils of Mentha piperita & Lavendula angustifolia on post-harvest phytopathogens. Commun AgricultureApple Biology Science, 71(3), 1321-6.
  6. Belkhadi, A., Hediji, H., Abbes, Z., Nouairi, I., Barhoumi, Z., Zarrouk, M., Chaibi, W. & Djebali, W. (2010). Effects of exogenous salicylic acid pre-treatment on cadmium toxicity and leaf lipid content in (Linum usitatissimum L.). Ecotoxicology and Environmental Safety, 73(5), 1004-1011.
  7. Clark, I. V. & Cameron, G. C.)2002(. An aroma chemical profile menthol. Perfumer & Flavorist, 23(4), 33-46.
  8. El-Quesni, F. E. M., kandil, M. M. & Mahgoub, M. H. (2007). Some studies on the effect of putrescine and paclobutrazol on the growth and chemical composition of Bougainillea glabra L. at Nuboria. American–Eurasian Journal of Agriculture and Envirment Science, 2(5), 552-558.
  9. De Sousa, A A ., Soares, P. M., de Almeida, A. N., Maia,  A. R., de Souza, E. P. & Assreuy, A. M. (2010). Antispasmodic effect of Mentha piperita L essential oil on tracheal smooth muscle of rats. Journal Ethnopharmacol, 130(2), 433-436.
  10. Eraslan, F., Inal A., Gunes, A. & Alpaslan, M. (2007). Impact of exogenous Salicylic acid on the growth, antioxidant activity and physiology of carrot plant subjected to combined salinity and boron toxicity. Scientia Horticulturae, 27, 287-298.
  11. Eteghad, S., Mirzaei, H. Pour, S. & Kahnamui, S. (2009). Inhibitory Effects of Endemic Thymus vulgaris and Mentha piperita Essential oils on Escherichia coli O157: H7. Research Journal of Biological Sciences, 4(3), 340-344.
  12. Fariduddin, Q., Hayat, S. & Ahmad, A. (2003). Salicylic acid influences net photosynthetic rate, carboxylation efficiency, nitrate reductase activity and seed yield in Brassic juncea. Photosynthetica, 41(2), 281-284.
  13. Feiwen, P. F., Chen, J. Y., Kong, W. F., Pan, Q. H., Wan, S. B. & Huang, W. D. (2005). Salicylic acid induced the expression of phenylalanine ammonia-lyase gene in grape berry. Plant Science, 169(5), 928-934.
  14. Galeottia, N. D., Cesare Mannellia, L., Mazzantib, G., Bartolinia, A. & Ghelardini, C. (2002). Menthol: a natural analgesic compound. Neuroscience Letters, 322(5), 145-148.
  15. Gharib, F. A. L. (2006). Effect of salicylic acid on the growth, metabolic activites and oil content of basil and majoram. International Journal of Agriculture and Biology, 4, 485-492.
  16. Grieve, M. (1974). A modern herbal. London: Jonathan Cape, pp 553-543.
  17. Hashemi, Sh., Esrare, Z.&  Pourseyedi, Sh. (2010). Effect of seed priming by Salicylic acid the growth, some physiological characters and biochemical of the King (Lepidum sativum L). Plant Biology, 2(2), 1-10. (in Farsi)
  18. Horvath, E., Szalai, G. & Janda, T. (2007). Induction of abiotic stress tolerance by salicylic acid signaling. Journal PlantGrowth Regulation, 26, 290-300.
  19. Hyodo, H.&  Yang, Sh. (1997). Ethylene enhance synthesis of phenylalanine ammonialyase in pea seedlings. Plant Physiology, 47, 765-770.
  20. Javaheri, M., Mashayekhi, K., Dadkhah, A. & Zaker Tavallaee, F. )2012(. Effects of Salicylic acid on Yield and quality characters of tomato fruit (Lycopersicum esculantum Mill). International Journal of Agriculture and Crop Science, 4, 1184-1187.
  21. Kaydan, D., Yagmur, M.&  Okut, N. )2007). Effects of salicylic Acid on the Growth and some physiological characters in salt stressed wheat )Triticum aestivum L.) . Tarim Bilimleri Dergisi, 13(2), 114-119.
  22. Khan, W., Prithviraj, B. & Smith, D. L) .2003(. Photosynthetic responses of corn and soybean to foliar application of salicylates. Journal of Plant Physiology, 160, 485-492.
  23. Kim, J. S., Lee, B. H., Kim, S. H., Ok, K. H. &  Cho, K. Y. (2006) Response to environmental and chemical signals for anthocyanin biosynthesis in nonchlorophyllous corn (Zea mays L.) leaf. Journal of Plant Biology, 49, 16-25.
  24. Kong, J., Chia, L., Goh, N., Chia, T. &  Brouillard, R. (2003). Analysis and biological activities of anthocyanins. Phytochemistry, 64, 923-933.
  25. Khorshid, M. (2013). Effect of putrescine on antioxidative properties in Matricaria chamomilla. International Journal of Farming and Allied Sciences, 612-617.
  26. Kumar, A., Samarth, R. M. & Yasmeen, S. (2004). Anticancer and radioprotective potentials of Mentha piperita L. BioFactors, 22(1-4), 87 - 91.
  27. Ma, J.Y., Zhou, R. & Cheng, B. S. (1996). Effect of spermine on the peroxidase activity of detached wheat leaves. Journal Shandang Agriculture.Univversity, 27, 176-180.
  28. Mahgoub, M. H., Abb El Aziz, N. G. & Mazhar, A. M. A. (2011). Response of Dahlia pinnata L. plant to foliar spray with putrescine and thiamine on growth, flowering and photosynthetic pigments. Journal Agriculture and Enviroment Science, 10(5), 769-775.
  29. Malik, A.U., Singh, Z. & Dhaliwal, S. S. (2003). Exogenous application of putrescine affects mango fruit quality and shelf life. Acta Horticulturae, 628, ISSN 0567-7572.
  30. Mendoza, A. B., Godina, F. R., Torres, V. R., Rodriguez, H. R., & Maiti, R. K. (2002). Chilli seed treatment with salicylic and sulfosalicylic acid modifies seedling epidermal anatomy and cold stress tolerance. Crop Research, 24, 19-25.
  31. Moharekar, S.T., Lokhande, S. D., Hara, T., Tanaka, R., Tanaka A. & Chavan, P. D. (2003). Effect of salicylic acid on chlorophyll and carotenoid contents of wheat and moong seedlings. Photosynthetica, 41(2), 315-317.
  32. Movahed, N., Eshghi, S., Tafazoli, E. & Jamali, B. 2012. Effects of polyamins on vegetative characteristics, growth, flowering and yield of strawberry ('Paros' and 'Selva'). Acta Horticulturae, 926, 287-293.
  33. Nanvakenary, R., Moradi, H. &. Ghasemiomran, S. (2013). Effects of putrescine on morphological and physiological characteristics of ornamental plant African violet (Saintpaulia ionantha). Bulletin of Environment, Pharmacology and Life Sciences, 2(10), 118-122.
  34. Popova, L., Ananieva, V., Hristova, V., Christov, K., Geovgieva, K., Alexieva, V. & Stoinova, Z. (2003). Salicylic acid and methyl jasmonate- induced protection on photosynthesis to paraquat oxidative stress. Bulgarian Journal of Plant Physiology (Special issue) 133-152.
  35. Qinghua, S. H. & Zhujun, Z. (2008) Effect of exogenous salicylic acid on manganese toxicity, element contents and antioxidative system in cucumber. Environmental and Experimental Botany, 63, 317-326.
  36. Rangana, S. (1977). Mannual for analysis of fruit and vegetable products. Total McGraw Hill Publishing Company, New Delhi. pp. 73-76.
  37. Raskin, I. (1992). Salicylate, a new plant hormone. Plant Physiology, 99(3), 799-803.
  38. Rossi, A., Serraino, I., Dugo, P. D., Paola, R., Mondello, L., Genovese, T., Morabito, D., Dugo, G., Sautebin, L., Caputi, A. P. & Cuzzocrea, S. (2003). Protective effects of anthocyanins from blackberry in a rat model of acute lung inflammation. FreeRadical Research, 37, 891-900.
  39. Rowshan, V., Khoshkhoi, M. & Javidnia, K. (2010). Effects of salicylic acid on quality and quantity of essential oil components in Salvia macrosiphon. Journal Bioligy Environment Science, 4(11), 77-82.
  40. Seun-Ah, Y., Sang-Kyung, J., Eun-Jung, L., Chang-Hyun, S. & In-Seon, L. (2010). Comparative study of the chemical composition and antioxidant activity of six essential oils and their components. Natural Product Reports, 24(2), 140-151.
  41. Singh, B. & Usha, K. (2003). Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress. Plant Growth Regulation, 39, 137-141.
  42. Sood, S. & Nagar, P. K. (2008). Post-harvest alteration in polyamins and ethylene in two diverse rose species. Acta Physiology Plant, 30, 243-248.
  43. Srivastava, M. K. & Dwivedi, U. N. (2000). Delayed ripening of banana fruit by salicylic acid. Plant Science, 158, 87-96.
  44. Talaat, I. M., Bekheta, M. A. & Mahgoub, M. H. (2005). Physiological response of periwinkle plants (Catharanthus roseus L.) to tryptophan and putrescine. International Journal of Agricultural and Biological, 7, 210-213
  45. Talaat, I. M. & Balbaa, L. K. (2010). Physiological response of sweet basil (Ocimum basilicum L.) to putrescine and trans-cinnamic acid. American-Eurasian Journal of Agriculture and Environment Science, 8(40), 438-445.
  46. Tang, W. & Newton, J. R. (2005). Polyamines reduced salt induced oxidative damage by increasing the activities of antioxidant enzymes and decreasing lipid peroxidation in Virginia pine. Plant Growth Regulation, 46, 31-43.
  47. Taiz, L. & Zeiger, E. (2006). Plant Physiology. 4th Edition. Sinauer Associates Inc. Sunderland, Massachusetts. USA. P: 456.
  48. Trease, G. E. & Evans, W. C. (1983). Pharmacognosy. 12th ed. Eastbourne: Bailliere Tindall, pp 421-424.
  49. Valero, D., Vicente, A. P., Romero, D. M., Castilo, S. & Guillen, F. S. (2002). Plum storability improved after calcium and heat postharvest treatments. Journal of Food Science, 67, 2571-2575.
  50. Valmorbida, J. & Boaro, C. S. F. (2007). Growth and development of Mentha piperita L. in nutrient solution as affected by rates of potassium. Brazilian Archives of Biology and Technol, 50(3), 379-84.
  51. Wagner, G. J. (1979). Content and vacuole/extra amino acids and anthocyanins in protoplast, vacuole distribution of neutral sugars. Free Plant Physiology, 64, 88-93.
  52. Yildirim, E. & Dursun, A. (2009). Effect of foliar salicylic acid application in plant growth and yield of tomato under greenhouse condition. Acta Horticulture, 807, 395-400
  53. Youssef, A. A., Mahgoub, M. A. & Talaat, I. M. (2004). Physiological and biochemical aspects of Matthiola incana L. plants under the effect of putrescine and Kinetin treaments. Egyption Journal of Applied Science, 19, 492-510.
  54. Zawoznik, M. S., Gropp, M. D., Tomaro, M. & Benavides, M. P. (2007). Endogenous salicylic acid potentiates cadmium- induced oxidative stress in Arabidopsis thaliana. Plant Science, 173, 190-197.
  55. Zhu, J. K. (2001). Cell signaling under salt, water and cold stresses. Plant Biology, 4(1), 401-406.