نقش براسینواستروئید روی ویژگی‌های بیوشیمیایی و کیفی میوۀ پرتقال واشنگتن ‌ناول در دورۀ انبارداری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، پژوهشکدۀ باغبانی، دانشگاه شهید باهنر کرمان، ایران

2 استادیار، پژوهشکدۀ باغبانی، دانشگاه شهید باهنر کرمان، ایران

چکیده

در این پژوهش به بررسی تأثیر براسینواستروئید بر ویژگی‌های بیوشیمیایی و کیفی میوۀ پرتقال ’واشنگتن ناول‘ در دورۀ انبارداری پرداخته شد. بدین منظور، میوه‌های پرتقال با غلظت‌های 0 (شاهد)، 75/0 و 5/1 میلی­گرم بر لیتر براسینواستروئید تیمار و در دمای 3±1 درجۀ سلسیوس و رطوبت نسبی 90-85 درصد به مدت پنج ماه نگهداری شدند و ویژگی­هایی مانند سرمازدگی، نشت یون، پراکسیداسیون ­لیپیدها، پراکسید هیدروژن، مواد جامد محلول، اسیدهای آلی، pH، اسید­آسکوربیک و فعالیت آنزیم­های پاداکسندگی (آنتی­اکسیدانی) پراکسیداز و کاتالاز در دورۀ انبار­داری ارزیابی شدند. نتایج نشان داد، کاربرد براسینواستروئید نسبت به شاهد، به‌طور معنی‌داری آسیب سرمازدگی، نشت یون، پراکسیداسیون لیپیدها و پراکسید ­هیدروژن را کاهش داد. بنا بر این نتایج، مواد جامد محلول کل، pH و اسید­آسکوربیک میوه­های تیمارشده و شاهد در دورۀ انبارداری روند افزایشی و میزان اسیدهای آلی روند کاهشی داشتند، اما استفاده از تیمار براسینواستروئید، تغییرپذیری دو روند یادشده را نسبت به شاهد کمتر تغییر داد. همچنین فعالیت آنزیم­های پاداکسندگی میوه­های تیمارشده با براسینواستروئید نسبت به شاهد، بسیار افزایش یافت. بنابر نتایج به‌دست‌آمده از این پژوهش، میوه‌های تیمارشده با 5/1 میلی­گرم بر لیتر براسینواستروئید، بالاترین کیفیت و بیشترین فعالیت آنزیم­های پاداکسندگی با کمترین آسیب سرمازدگی را داشتند.

کلیدواژه‌ها

عنوان مقاله [English]

Role of brassinosteroid on biochemical and qualitative characteristics of ‘Washington Navel’ orange fruit during storage

نویسندگان [English]

  • Bahareh Ghorbani 1
  • Zahra Pakkish 2
  • Masoud Khezri 2
1 M. Sc. Student, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
2 Assistant Professor, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
چکیده [English]

Orange fruits were treated with 0 (control), 0.75 and 1.5 mgL-1 brassinosteroid for 5 Min and then stored at 3±1°C and 85-90 % relative humidity for 5 month. Characteristics of chilling injury, ion leakage, lipid peroxidation, hydrogen peroxide, total soluble solids, titratable acidity, pH, ascorbic acid, and activity of antioxidant enzymes (peroxidase and catalase) have been evaluated. Results showed that fruits treated with brassinosteroid significantly reduced the chilling injury, ion leakage, lipid peroxidation and hydrogen peroxide, compared to control. According this results, total soluble solids, pH, and ascorbic acid, of treated and control fruits increased during storage while titratable acidity of fruits decreased. The pattern of mentioned changes reduced in brassinosteroid treatments lower than to control. The activity of antioxidant enzymes of fruits treated with brassinosteroid highly increased compared to control during storage. Fruits treated with 1.5 mgL-1 brassinosteroid showed highest quality and activity of antioxidant enzymes, with lowest chilling injury.

کلیدواژه‌ها [English]

  • antioxidant enzyme
  • brassinosteroid
  • orange
  • qualitative characteristics storage

مراجع

  1. Aghdam, M­. ­S., Asghari, M., Farmani, B., Mohayeji, M. & Moradbeygi, H. (2012). Impact of postharvest brassinosteroids treatment on PAL activity in tomato fruit in response to chilling stress. Scientia Horticulturae, 144, 116-120.
  2. Basiouny, F.­­­ M. (1996).­ Blueberry fruit quality and storability influenced by postharvest application of polyamines and heat treatments. Proccding Fland State Horticulturae Society, 109, 269-272.
  3. Bradford, M.­ M. (1976). ­A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.
  4. Burdurlu, H.­ S., Nuray, K. & Feryal, K. (2006).­Degradation of vitamin C in citrus juice concentrates during storage. Journal of Food Engineering, 74, 211-216.
  5. Cao, S.­ F. & Zheng, Y. ­H. (2008).­ Postharvest biology and handling of loquat fruit. Stewart Postharvest Review, 4, 1-5.
  6. Cioroi, M. ­(2007).­Study on L-ascorbic acid contents from exotic fruits. Cercetari Agronomicin Moldova, 1, 23-27.
  7. Chang, K. (1992).­The evaluation of Citrus demand and supply. In: Proceeding of International Society Citric. Italy, 3, 1153-1155.
  8. Clous, S.­ D. & Sasse, M. (1998).­Brassinosteroids: essential regulators of plant growth and development. Annual Physiology Reviews, 49, 427- 451.
  9. Dhindsa, R. S., Dhindsa, P. & Thorpe, A. T. (1981). ­Leaf senescence correlated with increased levels of membrane permeability and lipid peroxidation and decrease levels of superoxide dismutase and catalase. Journal Experimental Botany, 32, 93-101.
  10. Enteshari, S., Kalantari, K. & Ghorbani, M. (2006).­The effect of epibrassinosteroid and different bands of cultra violent radiation on the pigments content in glycine max. Pakistan Journal of Biological Science, 9, 231-237. 
  11. FAO. (2014).­ Food and Agriculture Organization of the United Nations Website. in: http:// www.faosat.org.
  12. Forney, C.­F. & Peterson S.­J. (1990).­Chilling induced potassium leakage of cultured Citrus cells. Physiology Plant, 78, 193-196
  13. Fotouhi-Ghazvini, R. & Fattahi Moghadam, J. (2003). Citrus growing in Iran. The University of Guilan Press.305p.(in Farsi)
  14. Heath, R. ­L. &­ Packer, L.­ (1969).­Photoperoxidation in isolated chloroplast, kinetics and stoichiometry of fatty acid peroxidation.  Archive of Biochemistry and Biophysiology, 125, 189-198
  15. Kelebek, H., Selli, S., Canbas, A. & Cabaroglu, T. (2009). HPLC determination of organic acids, sugars, phenolic composition and antioxidant capacity of orange wine made from a Turkish cv. Kozan. Microchemical Journal, 91, 187-192.
  16. Khripach, V., Zhabinskii, V. & Groot, A. D. (1998). Brassinosteroids: a new class of plant hormones. Academic Press. United States of Amenca. Pp. 460-469.
  17. Kochba, J., Lavee, S. & Spiegel-Roy, P. (1977). Differences in peroxidase activity and isoenzymes in embryogenic and non-embryogenic ‘Shamouti’ orange ovular callus lines. Plant and Cell Physiology, 18, 463-497.
  18. Manners, G. ­D., Breksa, A. ­P., Schoch, T.­ K. & Hidalgo, M. ­B. (2003). Analysis of bitter limonoids in citrus juices by atmospheric pressure chemical ionization and electrospray ionization liquid chromatography-mass spectrometry. Journal Agricultural and Food Chemistry, 51, 3709-3714.
  19. Montesinos-Herrero,­ C. & Palou, L. (2010). Combination of physical and low-toxicity chemical postharvest treatments for the management of citrus fruit: a review. Stewart Postharvest Review, 1, 11-225.
  20. Nour, V., Trandafir, I. & Ionica, M.­ E. (2010). HPLC organic acid analysis in different citrus juice under reversed phase conditions. Note Botanical and Horticulturae Agrobotanic, 38, 44-48.
  21. Nilprapruck, P., Authanithee, F. & Keebjan, P. (2008). Effect of exogenous methyl jasmonate on chilling injury and quality of pineapple. Silpakorn University Science and Techolog, 2, 33-42.
  22. Obenland, D., Collin, S., Sievert, J. & Arpaia, M.­ L. (2012). Impact of high-temperature forced-air heating of navel oranges on quality attributes, sensory parameters, and flavor volatiles. HortScience, 47, 386-390.
  23. Odriozola-Serrano, I., Hernndez-Jover, T. & Martn-Belloso, O. (2007). Comparative evaluation of UV–HPLC methods and reducing agents to determine vitamin C in fruits. Food Chemistry, 105, 1151-1158.
  24. Pantastico, E.­ B., Soule, J. & Grierson, W. (1968). Chilling injury in tropical and subtropical fruits: II. limes and grapefruit. Proceeding of Tropical Region American Society. Horticultural Science, 12, 171-183.
  25. Pipattanawong, N., Fujishige, N., Yamane, K. & Ogata, R. (1996). Effect of brassinosteroid on vegetative and reproductive growth in two day-neutral strawberries. HortScience, 65, 651-654.
  26. Purvis, A.­ C. (1985). Relationship between chilling injury of grape fruit and moisture loss during storage. Amelioration by polyethylene shrink film. Journal of American Society Horticultural Science, 110, 385-388.
  27. Sairam, R. K., Deshmukh, P.S. & Shukla, D.S. (1997). Tolerance to drought and temperature stress is relation to increased antioxidant enzyme activity in Wheat. Journal of  Agronomy Crop Science, 178, 171-177
  28. Schirra, M. & D’hallewin, G. (1997). Storage performance of Fortune mandarins following hot water dips. Postharvest Biology and Technology, 10, 229-238.
  29. Schirra, M., Mulas, M., Fadda, A., Mignani, I. & Lurie, S. (2005). Chemical and quality traits of ‘Olinda’ and ‘Campbell’ oranges after heat treatment at 44 or 46-C for fruit fly disinfestations. Lebenson. Wiss. Technology, 38,519-527.
  30. Serrano, M., Martinez-Romero, D., Guillen, F. & Valero, D. (2003). Effects of exogenous putrescine on improving shelf life of four plum cultivar. Postharvest Biology and Technology, 30, 259-271.
  31. Sha, S.­F., Li, J.­C. & Zhang, S.­L. (2011). Change in the organic acid content and related metabolic enzyme activities in developing Xinping pear fruit. African Journal of Agricultural Research, 6, 3560-3566.
  32. Shahbaz, M. & Ashraf, M. (2007). Influence of exogenous application of brassinosteroid on growth and mineral nutrients of wheat under saline conditions. Plant Physiology, 143, 513-522.
  33. Skog, L. J. & Chu, C.­ L. (2001). Effect of ozone on qualities of fruits and vegetables in cold storage. Canadian Journal of Plant Science, 81, 773-778.
  34. Slaughter, D.­ C., Obenland, D. ­M., Thompson, J. ­F., Arpaia, M. ­L. & Margosan, D.­ A. (2008). Non-destructive freeze damage detection in oranges using machine vision and ultraviolet fluorescence. Postharvest Biology and Technology, 48, 341-346.
  35. Velikova, V., Yordanov, I. & Edreva, A. (2000). Oxidaive stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Science, 151, 59-66.
  36. Villiers, F., Jourdain, A., Bastien, O., Leonhardt, N., Fujioka, S., Tichtincky, G., Parcy, F., Bourguignon, J. & Hugouvieux, V. (2012). Evidence for functional interaction between brassinosteroids and cadmium response in Arabidopsis thaliana. Journal Exprimental Botany, 10, 335-346.
  37. Xia, X. ­J., Wang, Y. J., Zhou, Y. H., Tao, Y., Mao, W. H., Shi, K., Asami, T., Chen, Z. & Yu, J. Q. (2009). Reactive oxygen species are involved in brassinosteroid-induced stress tolerance in cucumber. Plant Physiology, 150, 801-814.
  38. Zhang, J. & Kirkham, M.­ B. (1996). Enzymatic responses of the ascorbate-glutathione cycle to drought in sorghum and sunflower. Plant Science, 113, 139-147
  39. Zhang, C. ­F. & Tian, S. P. (2010). Peach fruit acquired tolerance to low temperature stress by accumulation of linolenic acid and N-acylphosphatidylethanolamine in plasma membrane. Food Chemistry, 120, 864–872.
  40. Zhang, A., Zhang, J., Ye, N., Cao, J., Tan, M., Zhang, J. & Jiang, M. (2010). ZmMPK5 is required for the NADPH oxidase-mediated self-propagation of apoplastic H2O2 in brassinosteroid-induced antioxidant defense in leaves of maize. Journal of Experimental Botany, 61, 4399-4411.
  41. Zhu, Z., Zhang, Z., Qin, G. & Tian, S. P. (2010). Effects of brassinosteroids on postharvest disease and senescence of jujube fruit in storage. Postharvest Biology and Technology, 56, 50-55.
  42. Zokaee-Khosroshahi, M.­ R. & Esna-Ashari, M. (2007). Postharvest putresciene treatments extend the storage- life of apricot (Prunus armeniaca L.) Etokhm–Sefid, fruit. Journal of Horticultural Science and Biotechnology, 82, 986-990.
  43. Zokae-khosroshahi, M.­ R., Esna-Ashari, M. & Ershadi, A. (2007). Effect of exogenous puterscine on post-harvest life of strawberry (Fragari ananassa Duch) fruit, cultivare selva. Scientia Horticulturae, 114, 27-32.

فایل ها

سابقه مقاله

  • تاریخ دریافت: 28 بهمن 1393
  • تاریخ بازنگری: 02 خرداد 1394
  • تاریخ پذیرش: 01 تیر 1394

هم رسانی

ارجاع به این مقاله

آمار