بررسی تأثیر تنش شوری بر برخی صفات فیزیولوژیکی و بیوشیمیایی توده‌های مختلف گیاه دارویی شنبلیله (Trigonella foenum- graecum L.)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، دانشکدۀ کشاورزی، دانشگاه فردوسی مشهد

2 استاد، دانشکدۀ کشاورزی، دانشگاه فردوسی مشهد

3 استادیار، دانشکدۀ کشاورزی، دانشگاه فردوسی مشهد

چکیده

شنبلیله (Trigonella foenum-graecum L.) از مهم‌ترین گیاهانی است که در صنایع دارویی استفاده می­شود. به‌منظور بررسی تأثیر شوری کلرید سدیم بر محتوای رطوبت نسبی، پایداری غشاء، محتوی سبزینه (کلروفیل)، سدیم، پتاسیم، نسبت سدیم به پتاسیم و انباشت متابولیت­های سازگاری (پرولین)، آزمایشی به‌صورت فاکتوریل در قالب طرح کامل تصادفی با سه تکرار به‌صورت گلدانی در سال 1392 اجرا شد. تیمارهای آزمایشی شامل هشت تودۀ بومی شنبلیله از اصفهان، تبریز، همدان، ساری، چالوس، آمل، مشهد، یاسوج و چهار سطح شوری (60، 120، 180 میلی­مولار و آب آشامیدنی به‌عنوان شاهد) بود. نتایج تجزیه­های آماری نشان داد که تنش شوری اثر معنی­داری بر محتوای رطوبت نسبی، پایداری غشاء، سبزینۀ a، سبزینۀ b، سبزینۀ کل، سدیم، پتاسیم و میزان پرولین داشت. با افزایش سطوح شوری آب آبیاری همۀ صفات به‌جز میزان پرولین، سدیم و نسبت سدیم به پتاسیم کاهش یافت. ازنظر صفات فیزیولوژیکی و بیوشیمیایی بیشترین میزان از گروه شاهد (شوری صفر) به دست آمد. بیشترین میزان سدیم، سدیم به پتاسیم و پرولین در آخرین سطح شوری (180 میلی­مولار) نسبت به شاهد مشاهده شد. بین توده­ها، به ترتیب توده­های چالوس، مشهد و اصفهان متحمل‌ترین توده­ها به شوری بودند. بر پایۀ نتایج این پژوهش، به نظر می­رسد بتوان از این سه توده به‌عنوان توده­های حاوی ژن­های متحمل به شوری برای اصلاح توده­هایی با ظرفیت عملکرد زیاد در مناطق شور استفاده کرد.

کلیدواژه‌ها

عنوان مقاله [English]

Investigation of the effects of salt stress on some physiological and biochemical characteristics of different landraces of fenugreek (Trigonella foenum - graecum L.)

نویسندگان [English]

  • Hassan Farhadi 1
  • Majid Azizi 2
  • Seyed Hossein Nemati 3
1 M. Sc. Student, College of Agriculture, Ferdowsi University of Mashhad, Iran
2 Professor, College of Agriculture, Ferdowsi University of Mashhad, Iran
3 Assistant Professor, College of Agriculture, Ferdowsi University of Mashhad, Iran
چکیده [English]

Fenugreek is one of the important medicinal plant that used in the pharmaceutical industry. In order to investigate the effects of salinity stress on relative water content, membrane stability, total chlorophyll, sodium, potassium, sodium to potassium ratio and proline, a factorial experiment was conducted in a completely randomized design with three replications in 2013. Experimental treatments were combination of eight fenugreek landraces (Isfahan, Tabriz, Hamedan, Sari, Challous, Amol, Mashhad and Yasooj) and four salinity levels (60, 120 and 180 mM NaCl and tap water as a control). Comparison of treatment means revealed significant differences among different salinity levels on relative humidity, membrane stability, chlorophyll a, chlorophyll b, total chlorophyll, sodium, and potassium and proline amount. Different levels of salinity led to significant reductions in all of measured in except of proline, sodium and sodium to potassium. The maximum amount of physiological and biochemical characteristics belonged to control (0 mM). The maximum of sodium, sodium to potassium and proline were observed in the salinity level of 180 mM compared to control. Between landraces, Challous, Mashhad, and Isfahan showed the highest amount in most of the studied characteristics in comparison with others. According to the results, these three landraces can be considered as the landraces which contains genes of salt tolerance that could be used to improve genotypes with high potential yield in salin condation.

کلیدواژه‌ها [English]

  • Chlorophyll
  • Environmental Stress
  • membrane stability
  • proline
  • relative water content (RWC)

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سابقه مقاله

  • تاریخ دریافت: 23 اسفند 1393
  • تاریخ بازنگری: 15 اردیبهشت 1394
  • تاریخ پذیرش: 29 اردیبهشت 1394

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