تغییرپذیری رنگدانه‌های نورساختی، اسید کلروژنیک، اسید کافئیک و پرولین پینة کنگرفرنگی تحت تأثیر کاربرد محرک‏های متابولیتی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، دانشکدۀ تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان

2 دانشیار، دانشکدۀ تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان

3 دانشیار دانشکدۀ صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان

چکیده

اسید سالیسیلیک (SA) و متیل جاسمونات (MeJA) به‌عنوان دو هورمون گیاهی با القای ساخت (سنتز) آنزیم فنیل آلانین آمونیالیاز و تحریک تولید و تجمع پروتئین کیناز­ها، اکسیژن­های رادیکال آزاد و اسمولیت­هایی مانند پرولین نقش بسزایی در تولید متابولیت­های ثانویه ایفاء می­کنند. در این پژوهش تأثیر محرک­های SA و MeJA بر تجمع امینواسید پرولین، محتوای اسید کلروژنیک و اسید کافئیک و همچنین غلظت رنگدانه‌های درونی پینة (کالوس) کنگرفرنگی بررسی شد. به این منظور نمونه‏های پینة کنگرفرنگی با پنج سطح 0، 50، 100، 200 و 250 میکرومولار SA و MeJA (الیسیتور) با چهار تکرار، در قالب طرح کامل تصادفی تیمار شدند. بر پایۀ نتایج به‌دست‌آمده تجمع پرولین و محتوای اسید کلروژنیک و اسید کافئیک تحت تأثیر غلظت‌های مختلف محرک‏های به‌کاربرده‌شده قرار داشتند. نکتۀ جالب‌توجه اینکه بین این ترکیب‌ها و تأثیر محرک‏های مورداستفاده همبستگی مشاهده شد. به‌طوری‌که تحت تأثیر تیمار 100 میکرومولار SA بیشترین تجمع اسید کلروژنیک، اسید کافئیک و پرولین مشاهده شد. افزایش سطح محرک­ها از این مقدار،کاهش محتوای اسید کلروژنیک، اسید کافئیک و میزان پرولین را به همراه داشت. در نمونه‌های تیمارشده با MeJA نیز نمونه­های تیمارشده با متیل جاسمونات 100 میکرومولار بیشترین محتوای اسید کافئیک و پرولین را داشته و بیشترین میزان اسید کلروژنیک در تیمار 200 میکرومولار تجمع یافت همچنین بر پایۀ نتایج به‌دست‌آمده ممکن است بیان شود، اگرچه تولید متابولیت‏ها تحت تأثیر محرک‏های متابولیتی افزایش می‌یابند، بااین‌وجود افزایش غلظت محرک‌های متابولیتی بیش‌ازحد بهینه نتیجۀ عکس خواهد داشت.

کلیدواژه‌ها


عنوان مقاله [English]

Influence of elicitors on photosynthesis pigments, caffeic acid, chlorogenic acid and proline content of Cynara scolymus callus

نویسندگان [English]

  • Saba Samadi 1
  • Azim Ghasemnezhad 2
  • Mehdi Alizadeh 2
  • Mehran Alami 3
1 M.Sc. Student, Department of Plant production, Agricultural Sciences and Natural Resources University of Gorgan, Gorgan, Iran
2 Associate Professor, Department of Plant production, Agricultural Sciences and Natural Resources University of Gorgan, Gorgan, Iran
3 Associate Professor, Department of Food Science, Agricultural Sciences and Natural Resources, University of Gorgan, Gorgan, Iran
چکیده [English]

Salicylic acid (SA) and Methyl jasmonate (MeJA) as phytohormone regulate synthesis of secondary metabolites in a wide range of plant species by accumulation of phenylalanine ammonia lyase enzyme (PAL) and accelerate the production of oxygen free radicals, protein kinase, proline and nitrite oxide content. To study the effect of these compounds on synthesis of secondary metabolites, in two separate experiments the callus of artichoke was treated with different levels of MJ and SA (0, 50, 100, 200,250 µM) in the solid MS medium. A positive correlation between proline accumulation and phenolic compounds and negative correlation by photosynthesis pigments showed that in artichoke, the cells try to balance primary and secondary metabolites. Based on the obtained results, when the elicitors applied to cell culture, the amount of phenyl propanoid compounds and proline significantly increased. Application of SA at 100µM had the highest effect on the production of chlorogenic acid and caffeic acid as well as proline. And MJ in 100 µM had the maximum amount of caffeic acid and proline and highest amount of chlorogenic acid was seen in 200 µM. It can be concluded that poly phenolic compounds production, influenced by executed treatments. Optimization the elicitor concentrations could lead to desirable secondary metabolite production of artichoke under in-vitro conditions.

کلیدواژه‌ها [English]

  • Caffeic Acid
  • chlorogenic acid
  • Methyl jasmonate
  • proline
  • Salicylic Acid
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