نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی سابق کارشناسی ارشد، دانشکدة کشاورزی، دانشگاه ایلام
2 استادیار، دانشکدة کشاورزی، دانشگاه ایلام
3 دانشیار، مؤسسة تحقیقات اصلاح و تهیة نهال و بذر کرج
4 استادیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج
چکیده
به منظور ریزازدیادی چهار پایة پر رشد گلابی شامل پایههای کنجونی، Pyrus betulifolia، درگزی و پایة Gh1 اثر چند محیط کشت پایه (MS، QL و QL تغییریافته)، سایتوکینین و اکسین بر میزان باززایی، پرآوری و ریشهزایی آن‌ها در شرایط درون شیشهای در مؤسسة تحقیقات اصلاح و تهیة نهال و بذر کرج بررسی شد. نتایج نشان داد در بین محیط‌های کشت پایه، بیشترین میزان پرآوری برای همة پایهها، در محیط کشت QL مشاهده شد. در بین پایههای گلابی، پایة Gh1 در هر دو محیط کشت QL و QL تغییریافته، باززایی مطلوبی را نشان داد. نتایج مربوط به پرآوری ریزنمونهها در غلظت‌های 1 و 2 میلیگرم در لیتر BAP و 0، 1 و 2 میلیگرم در لیتر 2ip نشان داد، بیشترین پرآوری در غلظت 1 میلیگرم در لیتر BAP به دست آمد، اما با اضافه کردن 2ip به محیط کشت، تأثیر معنا‌داری بر پرآوری ریزنمونهها نداشت. همچنین درصد ریشهزایی پایهها با استفاده از غلظت‌های مختلف IBA نشان داد که بیشینه ریشهزایی در غلظت 1 میلیگرم در لیتر IBA به دست آمد. ارزیابی درصد ریشهزایی در دو محیط کشت حاوی آگار و پرلایت نشان داد که بیشترین ریشهزایی پایهها به‌غیراز پایة P. betulifolia، در محیط کشت حاوی پرلایت به دست آمد. درمجموع به منظور موفقیت در ریزازدیادی پایههای یادشده در شرایط درون شیشهای استفاده از محیط کشت QL حاوی BAP (1 میلیگرم در لیتر) برای پرآوری مطلوب و تیمار 1 میلیگرم در لیتر IBA در شرایط پرلیت برای ریشهزایی مطلوب، قابل توصیه است.
کلیدواژه‌ها
عنوان مقاله [English]
Optimization of in vitro micropropagation protocol for some vigorous rootstocks of pear
نویسندگان [English]
- Masumeh Mansouryar 1
- Javad Erfani Moghadam 2
- Hamid Abdollahi 3
- Seyed Alireza Salami 4
2 Assistant Professor of Department of Horticulture, Ilam University
چکیده [English]
In order to micropropagate of four vigorous rootstocks of pear, including Pyrus betulifolia, Konjuni, Dargazi and Gh1, effects of basic plant culture media (MS, QL and modified QL), cytokinins and auxins on productivity, proliferation and rooting were studied in Seed and Plant Improvement Institute, Karaj. The highest amount of productivity for all rootstocks was observed in QL medium. Among pear rootstocks, Gh1 rootstock in both QL and modified QL mediums showed proper growth. Results of proliferation (BAP with 1 and 2 mg/l and 2ip at 0, 1 and 2 mg/l) illustrated that the highest amount of proliferation was achieved with 1 mg/l BAP, while adding 2ip into medium had no significant influence on proliferation rate. In addition, maximum rooting percentage was obtained by IBA at 1 mg/l. Also, evaluation of rooting percentage in both culture media containing agar and perlite proved that the highest rooting of all rootstocks (except P. betulifolia) was achieved in culture medium containing perlite. In general, to succeed micropropagation of aforementioned rootstocks, utilizing QL culture medium containing BAP (1 mg/l) is recommended for optimal proliferation and treatment of IBA (1 mg/l) in perlite is suitable for proper rooting.
کلیدواژه‌ها [English]
- Auxin
- cytokinin
- Pear
- proliferation
- rooting
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