تأثیر تنظیم‌کننده‌های رشد گیاهی بر ریزازدیادی گیاه گزانگبین (Astragalus adscendens Boiss and Haussk) با جوانة انتهایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکدة کشاورزی، دانشگاه فردوسی مشهد

2 دانشیار، دانشکدة کشاورزی، دانشگاه فردوسی مشهد

3 استادیار، دانشکدة کشاورزی، دانشگاه فردوسی مشهد

4 استادیار پژوهشکدة علوم گیاهی، دانشگاه فردوسی مشهد

چکیده

گزانگبین (Astragalus adscendens)متعلق به جنس گون‌ها و یکی از گونه‌های پر­اهمیت ایران از نظر ارزش دارویی، جنبه‌های اقتصادی و اجتماعی است. به‌ دلیل قدرت افزایش کم این گیاه با بذر و قلمه، امکان کشت درون شیشه‌ای این گیاه ارزیابی شد. اثر سه نوع سیتوکینین مختلف (BAP1، KIN2 و Ze3) با غلظت‌های 5/0، 1، 2 و mg/l 4 در ترکیب با 0 و mg/l 5/0 NAA4 بر پرآوری جوانة انتهایی بررسی شد. آزمایش به صورت فاکتوریل در قالب طرح کامل تصادفی انجام شد. شاخساره‌‌ها به محیط کشت ریشه‌زایی حاوی IBA5 و NAA با غلظت‌های 0، 3/0، 6/0 و mg/l 9/0 از هر کدام منتقل شدند. نتایج نشان داد تأثیر کاربرد سیتوکینین‌های مختلف و غلظت‌های استفاده‌شده از هر کدام و همچنین کاربرد NAA در میزان شاخسارة تولیدشده، شمار برگ و طول شاخساره‌ها معنی‌دار بود. بیشترین شاخه‌زایی (5/8 شاخساره در هر ریزنمونه) به همراه شمار برگ مناسب (4/22 برگ در هر شاخساره) در محیط کشت MS6 حاوی mg/l 4BAP  به دست آمد. همچنین بیشترین مقدار ریشه‌زایی در محیط کشت MS حاوی mg/l 3/0 از هورمون NAA به دست آمد (100 درصد). بیش از 40 درصد گیاهچه‌های تولیدشده در گلدان حاوی مخلوط خاک، پیت و پرلیت (1:1:1) سازگار شدند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Study on the effect of plant growth regulations (PGRs) on micropropagation of gavane-gaz-angubin (Astragalus adscendens Boiss & Haussk) via apical meristem

نویسندگان [English]

  • Ghasem Esmaeili 1
  • Majid Azizi 2
  • Hosein Aroei 3
  • Leila Samiei 4
1
2
3
4
چکیده [English]

Gavane-gaz-angubin (Persian Manna) belongs to Astragalus genus (Leguminosae). This plant is one of the most important species in Iran for its medicinal value. Considering the low propagation rate of this plant from seed and cutting, the feasibility of in-vitro propagation of this plant was investigated. Effect of different types and concentrations of cytokinins such as BAP, KIN, and Ze (0.5, 1, 2 and 4 mg/l) in combination with NAA (0, 0.5 mg/l) were evaluated for production of apical bud in MS medium. Experiments were conducted in a factorial based on completely randomized design. Shoots were transferred to rooting medium and this medium contained different concentrations (0, 0.3, 0.6, 0.9 mg/l) of IBA or NAA. Results showed that not only the significant effect of different types and concentrations of cytokinins, but also confirmed effect of various concentrations of NAA on shoots number, leaf number and shoots length. Results indicated that MS medium containing 4 mg/l BAP lead to production of highest shoot number (8.5 shoots per explants) as well as the highest leaf number. The highest rooting production (100%) obtained in MS medium with 0.3 mg/l NAA, Likewise. More than 40 % of plants were adapted in pots with the mixture of soil, peat and perlite (1:1:1, v/v).

کلیدواژه‌ها [English]

  • Gavane gazi
  • manna
  • proliferation
  • rooting
  • Tissue culture

مراجع

  1. Ahmad, N. & Anis, M. (2007). Rapid Clonal Multiplication of a Woody Tree, Vitex negundo L. Through Axillary shoots Proliferation. Agroforestry Systems, 71 (3), 195-200.
  2. Azimi, M., Mesdaghi, M. & Farohpor, M. (2005). Study of the relationships of cyamophila dicora loginova population and vegetation parameters of Astragalus adscendens in feridounshahr. Isfahan. Journal of Science and Technology of Agriculture and Natural Research, 9 (3), 243-252. (in Farsi)
  3. Azimi, M. (2005(. Ecology and habitat management of Astragalus adscendens in Isfahan province (case study of feridoun shahr city). M.Sc. Thesis. Agriculture Science and Naturel Research, Sari University, Iran.
  4. 4.       Başalma, D., Uranbey, S., Gürlek, D. & Özcan, S. (2008). TDZ-induced plant regeneration in Astragalus cicer L. African Journal of Biotechnology, 7(8), 955-959.
  5. 5.       Behroznam, B., Khoshkhoei, M., Tafazoli, A. & Khalighi, A. (2008). Comparison effect of medium culture and plant growth regulations of proliferation and rooting of Jasminum grandiflorum in vitro culture condition. Journal of Research in Agricultural Science, 4(1), 83-92. (in Farsi)
  6. Esmaili, GH. & Azizi, M. (2013). Effect of different concentration of IBA on three types of gaz-e-angubin (Astragalus adscendens). In: 2nd National Congress on Medicinal Plant, 15-16 may. University of Shahid Beheshti, Tehran, Iran, pp, 610.
  7. Erisen, S., Yorgancilar, M., Atalay, E., Babaoglu, M. & Duran, A. (2010). Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey. Electronic Journal of Biotechnology, ISSN, 0717-3458.
  8. Farahnaky, A., Shojaei, Z.A., Sadeghi. Khomami, A. & Majzoobi, M. (2009). Physicochemical Properties and Rheological Behaviour of Gaz-Angubin. International Journal of Food Properties, 12, 347-357.
  9. Farsi, M. & Zolala, J. (2011). Introduction to plant biotechnology (5th ed.). Ferdowsi University of Mashhad Publication.
  10. Hou, S.W. & Jia, J.F. (2004). High Frequency Plant Regeneration from Astragalus melilotoides Hypocotyl and Stem Explants via Somatic Embryogenesis and Organogenesis. Plant Cell, Tissue and Organ Culture, 79 (1), 95-100.
  11. 11.    Hung, C.Y. & Xie, J. (2008). Development of an Efficient Plant Regeneration System for the Selenium-Hyperaccumulator Astragalus racemosus and the Nonaccumulator Astragalus Canadensis. Horticultural Science, 43(7), 2138-2142.
  12. 12.    Jalili Marandi, R., Naseri, L., Mohseniazer, M., Hajitagiloo, R. & Marhamati, M. (2011). Investigation on interaction effect of banzyladenine and chitosan on in vitro proliferation of strawberry (fragaria × ananassa cv. Selva). Agricultural biotechnology, 10 (1), 27-34. (in Farsi)
  13. Kardovani, P. (2005). Pastures in Iran problems and solutions (4th ed.). University of Tehran publication.
  14. Li, R.J., Qiu, S-D., Chen, H.X., Tian, H. & Wang, H.X. (2007). The Immunotherapeutic Effects of Astragalus Polysaccharide in Type 1 Diabetic Mice. Biological and Pharmaceutical Bulletin, 30(3), 470-476.
  15. Luo, J.P. & Jia, J.F. (1998). Plant Regeneration from Callus Protoplasts of the Forage Legume Astragalus adsurgens Pall. Plant Cell Reports, 17(6-7), 313-317.
  16. Luo, J.P., Jia, J-F., Gu, Y.H. & Liu, J. (1999). High Frequency Somatic Embryogenesis and Plant Regeneration in Callus Cultures of Astragalus adsurgens Pall. Plant Science, 143, 93–99.
  17. Masomi, A. (1994(. Astragalus of Iran. Research Institute of Forest and Rangeland Publication, Tehran.
  18. Mercier, H., Vieira, C.C.J. & Figueredo-Ribeiro, R.C.L. (1992). Tissue Culture and Plant Propagation of Gomphrena officinalis, a Brazilian Medicinal Plant. Plant Cell, Tissue and Organ Culture, 28 (3), 249-254.
  19. Mohamadi, M. & Deni, M. (2001). Identification of producing factors, how to produce and operation of manna in Iran. Iranian Journal of Medicinal and Aromatic Plants, 17, 79-118. (In Farsi)
  20. Puspashree, P. & Shiba, P.R. (2012). In Vitro Micropropagation of Desmodium Gangeticum (L.) DC (Fam- Fabaceae): A Medicinal Legume through Axillary Bud Multiplication. Pakistan Journal of Biological Sciences, 15(10), 477-483.
  21. Rout, G.R. (2005). Micropropagation of Clitoria Ternatea Linn. (Fabaceae) An Important Medicinal Plant. In Vitro Cellular and Developmental Biology-Plant, 41(4), 516-519.
  22. Rolston, M.P. (1978). Water Impermeable Seed Dormancy. The Botanical Review, 44(3), 365-960.
  23. Rios, JL. & Waterman, P.G. (1997). A review of the Pharmacology and Toxicology of Astragalus. Phytotherapy Research, 11 (6), 411-418.
  24. Samsam Shariat, H. & Moatar, F. (2003). Plants and natural drugs (4th ed.). Rozbahan-Tehran publication.
  25. Seifallahi, A. & Ebadi, R. (2002). Gaz psyllids species, their distribution and population densities in Isfahan province. Iranian Journal of Agricultural Science, 33 (2), 187-196. (in Farsi)
  26. Seifollahi, A. & Shafezade, SH. (2002). Rang managements and the need to protect from Astragalus adscendens Boiss as hast of Gaz psyllids. Iranian Journal of Range and Desert Research, 7(1), 231-240. (in Farsi)
  27. Steephen, M., Nagarajan, S. & Ganesh, D. (2010). Phloroglucinol and Silver Nitrate Enhances Axillary Shoot Proliferation in Nodal Explants of Vitex negundo L. –an Aromatic Medicinal Plant. Iranian Journal of Biotechnology, 8(2), 82-89.
  28. Sudha, G.C. & Seeni, S. (1994). In Vitro Multiplication and Field Establishment of Adhatoda beddomei CB Clarke, A Rare Medicinal Plant. Plant Cell Reports, 13, 203-207.
  29. Sahoo, Y. & Chand, K. (1998). Micropropagation of Vitex negundo L. a woody aromatic medicinal shrub through high-frequency axillary shoot proliferation. Plant Cell Reports, 18(3-4), 301-307.
  30. Turgut-Kara, N. & Ar, S. (2006). Micropropagation of Astragalus maximus willd. Biotechnology and Biotechnological Equipment, 20(1), 20-23.
  31. Uranbey, S. (2005). Thidiazuron Induced Adventitious Shoot Regeneration in Hyoscyamus niger. Biologia Plantarum, 49(3), 427-430.

  32. Vidoz, M. L. (2006). Tissue Culture OF Trifolium polymorphum, T. carolinianum, Adesmia latifolia, A. bicolor and Lotononis bainesii. Abstract of Thesis Presented to the Graduate School of the University of Florida in Partial Fulfillment of the Requirements for the Degree of Master of Science.

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سابقه مقاله

  • تاریخ دریافت: 19 بهمن 1392
  • تاریخ بازنگری: 19 آذر 1393
  • تاریخ پذیرش: 07 دی 1393

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