تأثیر تنظیم‌کننده‌های رشد گیاهی بر ریزازدیادی گیاه گزانگبین (Astragalus adscendens Boiss and Haussk) با جوانة انتهایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکدة کشاورزی، دانشگاه فردوسی مشهد

2 دانشیار، دانشکدة کشاورزی، دانشگاه فردوسی مشهد

3 استادیار، دانشکدة کشاورزی، دانشگاه فردوسی مشهد

4 استادیار پژوهشکدة علوم گیاهی، دانشگاه فردوسی مشهد

چکیده

گزانگبین (Astragalus adscendens)متعلق به جنس گون‌ها و یکی از گونه‌های پر­اهمیت ایران از نظر ارزش دارویی، جنبه‌های اقتصادی و اجتماعی است. به‌ دلیل قدرت افزایش کم این گیاه با بذر و قلمه، امکان کشت درون شیشه‌ای این گیاه ارزیابی شد. اثر سه نوع سیتوکینین مختلف (BAP1، KIN2 و Ze3) با غلظت‌های 5/0، 1، 2 و mg/l 4 در ترکیب با 0 و mg/l 5/0 NAA4 بر پرآوری جوانة انتهایی بررسی شد. آزمایش به صورت فاکتوریل در قالب طرح کامل تصادفی انجام شد. شاخساره‌‌ها به محیط کشت ریشه‌زایی حاوی IBA5 و NAA با غلظت‌های 0، 3/0، 6/0 و mg/l 9/0 از هر کدام منتقل شدند. نتایج نشان داد تأثیر کاربرد سیتوکینین‌های مختلف و غلظت‌های استفاده‌شده از هر کدام و همچنین کاربرد NAA در میزان شاخسارة تولیدشده، شمار برگ و طول شاخساره‌ها معنی‌دار بود. بیشترین شاخه‌زایی (5/8 شاخساره در هر ریزنمونه) به همراه شمار برگ مناسب (4/22 برگ در هر شاخساره) در محیط کشت MS6 حاوی mg/l 4BAP  به دست آمد. همچنین بیشترین مقدار ریشه‌زایی در محیط کشت MS حاوی mg/l 3/0 از هورمون NAA به دست آمد (100 درصد). بیش از 40 درصد گیاهچه‌های تولیدشده در گلدان حاوی مخلوط خاک، پیت و پرلیت (1:1:1) سازگار شدند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Study on the effect of plant growth regulations (PGRs) on micropropagation of gavane-gaz-angubin (Astragalus adscendens Boiss & Haussk) via apical meristem

نویسندگان [English]

  • Ghasem Esmaeili 1
  • Majid Azizi 2
  • Hosein Aroei 3
  • Leila Samiei 4
چکیده [English]

Gavane-gaz-angubin (Persian Manna) belongs to Astragalus genus (Leguminosae). This plant is one of the most important species in Iran for its medicinal value. Considering the low propagation rate of this plant from seed and cutting, the feasibility of in-vitro propagation of this plant was investigated. Effect of different types and concentrations of cytokinins such as BAP, KIN, and Ze (0.5, 1, 2 and 4 mg/l) in combination with NAA (0, 0.5 mg/l) were evaluated for production of apical bud in MS medium. Experiments were conducted in a factorial based on completely randomized design. Shoots were transferred to rooting medium and this medium contained different concentrations (0, 0.3, 0.6, 0.9 mg/l) of IBA or NAA. Results showed that not only the significant effect of different types and concentrations of cytokinins, but also confirmed effect of various concentrations of NAA on shoots number, leaf number and shoots length. Results indicated that MS medium containing 4 mg/l BAP lead to production of highest shoot number (8.5 shoots per explants) as well as the highest leaf number. The highest rooting production (100%) obtained in MS medium with 0.3 mg/l NAA, Likewise. More than 40 % of plants were adapted in pots with the mixture of soil, peat and perlite (1:1:1, v/v).

کلیدواژه‌ها [English]

  • Gavane gazi
  • manna
  • proliferation
  • rooting
  • Tissue culture
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