نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی سابق کارشناسی ارشد، دانشکدۀ کشاورزی، دانشگاه تبریز
2 دانشیار، دانشکدۀ کشاورزی، دانشگاه تبریز
چکیده
بهمنظور بررسی صفات مربوط به ریزغدهزایی درون شیشهای در سیبزمینی رقم آگریا در واکنش به استفاده از دو آنتیژیبرلین تریازولی، پاکلوبوترازول و یونیکونازول، دو آزمایش جداگانه، هر یک در پنج تیمار 0، 08/0، 16/0، 32/0 و 64/0 میلیگرم بر لیتر ماده و با چهار تکرار و در قالب طرح کامل تصادفی به اجرا درآمد. در این دو آزمایش صفات درصد ریزغدهزایی، طول و قطر ریزغدهها، وزن تر ریزغدهها و شاخساره، طول جوانههای رشدیافته روی ریزغده، طول میانگره، شمار گره و برگ بررسی شدند. درصد ریزغدهزایی، طول، قطر و وزن تر ریزغدهها با افزایش میزان تریازول مصرفی افزایش پیدا کرد. طول جوانههای رشدیافته روی ریزغده با افزایش میزان تریازولها کاهش یافت که ناشی از کاهش چشمگیر طول میانگره است. شمار گرهها و برگها نیز با افزایش غلظت آنتی ژیبرلین افزایش پیدا کرد. در شاخصهای مربوط به ریزغدهزایی گیاهان تیمارشده با پاکلوبوترازول و یونیکونازول، عملکرد بهتری نسبت به گیاهان شاهد داشتند که این امر میتواند نشاندهندۀ مؤثر بودن این مواد در ریزغدهزایی باشد. با توجه به یافتههای اخیر به نظر میرسد استفاده از پاکلوبوترازول و یونیکونازول در افزایش ریزغدهزایی و اندازۀ ریزغدهها و کاهش رشد رویشی شاخساره در سیبزمینی رقم آگریا مؤثر باشد.
کلیدواژهها
موضوعات
عنوان مقاله [English]
Optimization of in vitro microtuberization of potato (Solanum tuberosum L. cv. Agria) using paclobutrazole and uniconazol
نویسندگان [English]
- parisa ghadimi garkeroodi 1
- Fariborz Zaare Nahandi 2
- Alireza Motalebi Azar 2
- jaber panahandeh 2
- mohammad reza dadpour 2
چکیده [English]
In order to investigate the characteristics of in vitro microtuberization of potato cv. Agria in response to the two triazole anti-gibberellin, paclobutrazole and uniconazol, two independent experiment in five concentrations (0, 0.08, 0.16, 0.32, 0.64 mg/L) of each compound were carried out with four replications for each treatment in a completely randomized design. In this experiment percent of microtuberization, length and diameter of microtubers, fresh weight of microtubers and shoots, length of developed vegetative buds on microtubers, length of internodes, number of nodes and leaves were recorded. Percent of microtuberization, length and diameter of microtubers and fresh weight of microtubers were increased with increasing of triazole concentration. Length of internodes and length of developed vegetative buds on microtubers were reduced by increasing of triazole concentration. Number of nodes and leaves were increased with increasing the concentration of anti-gibberellin. For indicators of microtuberization, treated plants with paclobutrazole and uniconazol had better performance than control plants. It could be indicated the efficacy of these materials on microtuberization. According to our results, it seems that these chemicals could be effective for improving characteristics of in vitro microtuberization in potato cv. Agria.
کلیدواژهها [English]
- Anti-gibberellin
- internode length
- performance
- triazole
- vegetative growth
- Balamani, V. & Poovaiah, B. W. (1985). Retardation of shoot growth and promoting of tuber growth of potato plants by paclobutrazol. American Potato Journal, 62, 363- 369.
- Bandara, M. S., Tanino, K. K. & Waterer. D. R. 1998. Effect of size and timing of plant growth regulator treatment on grown and tuber yield in greenhouse grown Norland and Russet Burbank potatoes. Journal of Plant Growth Regulation, 17, 75-79.
- Barani, M., Akbari, N. & Ahmadi, H. (2013). The effect of gibberellic acid (GA3) on seed size and sprouting of potato tubers (Solanum tuberosum L.). Academic Journal, 8, 3898-3903.
- Chapman, H.W. (2006). Tuberization in the potato plant. Physiologia Plantarum, 11, 215-224.
- 5. Davis, T. D., Sankhla, N. & Upadhyaya, A. (1988). Triazole plant growth regulators. Horticultural Reviews, 10, 63-105.
- 6. Dobránszki, J., Magyar-Tábori, K. &Hudák, I. (2008). In vitro tuberization in hormone free system on solidified medium and dormancy of microtubers. Fruit, Vegetable and Cereal Science and Biotechnology, 2, 82-94.
- Fletcher, A., Gilley, A., Sankhla, N. & Davies, T. (2000). Triazoles as plant groth regulators and stress protectants. Horticultural Reviews, 24, 55-138.
- Hoque, M. E. (2010). In vitro tuberization in potato (Solanum tuberosum L.). Plant Omics Journal, 3, 7-11.
- Kamoutsis, A. P., Chronopoulou-Sereli, A.G. & Paspatis, E.A. (1999). Paclobutrazol affects growth and flower bud production in gardenia under different light regimes. HortScience, 34, 674-675.
- Mares, D., Marshner, H. & Krauss, A. (1981). Effect of gibberellic acid on growth and carbohydrate metabolism of developing tubers of potato (Solanum tuberosum). Physiologia Plantarum, 52, 267- 274.
- Medina, R., Burgos, A., Difranco, V., Moroginski, L. & Cenoz, P. (2012). Effect of chlorocholine and paclobutrazol on cassava (Manihot esculenta Crantz cv. Rocha) plant growth and tuberous root quality. Agriscince, 29, 51-58.
- Muthukumarasamy, M. & Panneerselvam, R. (1997). Triazoles induced protein metabolism in the salt stressed Raphanus sativus seedling. Journal of the Indian Botanical Society, 76, 39-42.
- Nistor, A., Campeanu, G., Atanasiu, N., Chiro, N. & Karacsonyi, D. (2010). Influence of potato genotypes on in vitro production of microtubers. Romanian Biotechnological Letters, 15, 5317- 5324.
- Paiva_Neto, V.B. & Campos_Otoni, W. (2003). Carbon sucrose and their osmotic potential in plant tissue culture; does it matter?. Scienthica Horticulture, 97, 193-202.
- Panneerselvam, R., Muthukumarasamy, M. & Karikalan, L. (1997). Triadimefon enhance growth and net photosynthesis rate in NaCl stressed plant of Raphanus sativus L. Photosynthetica, 34, 605-609.
- Rossini pinto, A., Deleo Rodrigues,T., Leeita, I & Barbosa, J. (2005). Growth retardant on development and ornamental quality of potted (Lilliput) Zinnia elegans Jacq. Scientia Agricola, 62, 337-345.
- Sankar, B., Jaleel, C.A., Kishorekumar, P., Somasondaram, R. & Panneerselvam, R. (2007). Effect of paclobutrazol on water stress amelioration through antioxidants and free radical scavenging enzymes in Arachis hypogaea L. Colloids and Surfaces B: Biointerfaces, 60, 229-235.
- Sharma, S., Chanemougasoundharam, A., Sarkar, D. & Pandey, S. K. (2004). Carboxylic acids affect induction, development and quality of potato (Solanum tuberosum L.) microtubers grown in vitro from single-node explants. Plant Growth Regulation, 44, 219-229.
- Simko, I. (1994). Effect of paclobutrazol on in vitro formation of potato micro tubers and their sprouting after storage. Biologia plantarum, 36, 15 -20.
- Tsegaw, T. & Hammes, P. (2003). Growth responses of potato (Solanum tuberosum) grown in hot tropical lowland to applied paclobutrazol: 2. Tuber attributes. New Zealand Journal of Crop and Horticulture Science, 33, 43- 51.
-
Xu, X., Van Lammeren, A.M., Vermeer. E. & Vreugdenhil, D. (1998). The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro. Plant Physiology, 117, 575-584.