نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی سابق کارشناسی ارشد، دانشکدۀ کشاورزی، دانشگاه تبریز
2 دانشیار، دانشکدۀ کشاورزی، دانشگاه تبریز
چکیده
به‌منظور بررسی صفات مربوط به ریزغدهزایی درون شیشه‌ای در سیبزمینی رقم آگریا در واکنش به استفاده از دو آنتی‌ژیبرلین تریازولی، پاکلوبوترازول و یونیکونازول، دو آزمایش جداگانه، هر یک در پنج تیمار 0، 08/0، 16/0، 32/0 و 64/0 میلی‌گرم بر لیتر ماده و با چهار تکرار و در قالب طرح کامل تصادفی به اجرا درآمد. در این دو آزمایش صفات درصد ریزغدهزایی، طول و قطر ریز‌غده‌ها، وزن تر ریز‌غده‌ها و شاخساره، طول جوانه‌های رشدیافته روی ریزغده، طول میان‌گره، شمار گره و برگ بررسی شدند. درصد ریزغدهزایی، طول، قطر و وزن تر ریز‌غده‌ها با افزایش میزان تریازول مصرفی افزایش پیدا کرد. طول جوانه‌های رشدیافته روی ریزغده با افزایش میزان تریازول‌ها کاهش یافت که ناشی از کاهش چشمگیر طول میان‌گره است. شمار گره‌ها و برگ‌ها نیز با افزایش غلظت آنتی ژیبرلین افزایش پیدا کرد. در شاخص‌های مربوط به ریزغدهزایی گیاهان تیمارشده با پاکلوبوترازول و یونیکونازول، عملکرد بهتری نسبت به گیاهان شاهد داشتند که این امر می‌تواند نشان‌دهندۀ مؤثر بودن این مواد در ریزغدهزایی باشد. با توجه به یافته‌های اخیر به نظر می‌رسد استفاده از پاکلوبوترازول و یونیکونازول در افزایش ریزغدهزایی و اندازۀ ریز‌غده‌ها و کاهش رشد رویشی شاخساره در سیبزمینی رقم آگریا مؤثر باشد.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
Optimization of in vitro microtuberization of potato (Solanum tuberosum L. cv. Agria) using paclobutrazole and uniconazol
نویسندگان [English]
- parisa ghadimi garkeroodi 1
- Fariborz Zaare Nahandi 2
- Alireza Motalebi Azar 2
- jaber panahandeh 2
- mohammad reza dadpour 2
چکیده [English]
In order to investigate the characteristics of in vitro microtuberization of potato cv. Agria in response to the two triazole anti-gibberellin, paclobutrazole and uniconazol, two independent experiment in five concentrations (0, 0.08, 0.16, 0.32, 0.64 mg/L) of each compound were carried out with four replications for each treatment in a completely randomized design. In this experiment percent of microtuberization, length and diameter of microtubers, fresh weight of microtubers and shoots, length of developed vegetative buds on microtubers, length of internodes, number of nodes and leaves were recorded. Percent of microtuberization, length and diameter of microtubers and fresh weight of microtubers were increased with increasing of triazole concentration. Length of internodes and length of developed vegetative buds on microtubers were reduced by increasing of triazole concentration. Number of nodes and leaves were increased with increasing the concentration of anti-gibberellin. For indicators of microtuberization, treated plants with paclobutrazole and uniconazol had better performance than control plants. It could be indicated the efficacy of these materials on microtuberization. According to our results, it seems that these chemicals could be effective for improving characteristics of in vitro microtuberization in potato cv. Agria.
کلیدواژه‌ها [English]
- Anti-gibberellin
- internode length
- performance
- triazole
- vegetative growth
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