ارزیابی تأثیر قارچ‌ریشة آرباسکولار و باکتری‌های محرک رشد در شرایط تنش کم‌آبی بر عملکرد گیاه زینتی استئوسپرموم (Osteospermum hybrida ‘Passion Mix’)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، دانشگاه تهران، پردیس کشاورزی و منابع طبیعی، گروه مهندسی علوم باغبانی و فضای سبز، تخصص: فیزیولوژی گل و گیاهان زینتی/ تغذیه گیاهی/ کشت بافت/ بسترهای کشت/ گیاهان زینتی باغچه ای و گلخانه ای/ شمعدانی/ جعفری/ ارکیده/ لیلیوم

2 استادیار گروه علوم باغبانی پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

3 دانش آموخته سابق کارشناسی ارشد گروه علوم باغبانی پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

4 استادیار موسسه تحقیقات خاک و آب کشور، بخش بیولوژی خاک، کرج، ایران

چکیده

در این بررسی مشخص شد که قارچ‌ریشة (میکوریز) آربسکولار گونة Glomus mossea CA همزیستی بهتری با گیاه زینتی استئوسپرموم (Osteospermum hybrida ‘Passion Mix’) در مقایسه با گونه­های دیگر این قارچ دارد. در این پژوهش تأثیر این‌گونه از قارچ‌ریشة آربسکولار و باکتری­های محرک رشد به تنهایی و یا در ترکیب، در شرایط تنش کم‌آبی بر میزان استقرار (کلون‌سازی) در ریشه و شاخص‌های کمی و کیفی رشد و میزان فسفر در بافت گیاه بررسی شد. آزمایش در شرایط گلخانه‌ای به صورت فاکتوریل و در قالب طرح بلوک‌های کامل تصادفی در سه تکرار در طی دو سال انجام گرفت. نتایج نشان داد گیاهان تلقیح‌شده با قارچ‌ریشه رشد بیشتری در مقایسه با گیاهان تلقیح نشده داشتند. بنابر نتایج، این ریزموجود تنها در سطح آبیاری70درصد ظرفیت زراعی قادر به بهبود این شاخص‌ها در گیاه بود و در سطوح آبیاری 40درصد ظرفیت زراعی هرچند که استئوسپرموم توانست به رشد خود ادامه دهد اما بهره‌گیری از این ریزموجود نقش مؤثری در بهبود رشد گیاه نداشت. همچنین تأثیر هم‌افزایی بین قارچ و باکتری‌های محرک رشد مشاهده شد بنابراین به‌کارگیری قارچ‌ریشه و باکتری‌های محرک رشد و استفاده از سطح بهینة آبیاری،‌ می‌تواند در بهبود شاخص‌های رشد گیاه استئوسپرموم سودمند باشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effects of arbuscular mycorrhizal fungus and plant growth promoting rhizobacteria (PGPR) under drought stress on growth of ornamental osteospermum (Osteospermum hybrida ‘Passion Mix’)

نویسندگان [English]

  • Azizollah Khandan-Mirkohi 1
  • Mohammadreza Taheri 2
  • Forough Zafar-Farrokhi 3
  • Farhad Rejali 4
2 Asist. Prof. Dep. of Horticulture Sciences, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 Former MSc., Dep. of Horticulture Sciences, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
4 Asist. Prof. Soil and Water Research Institute, Karaj, Iran
چکیده [English]

Effects of arbuscular mycorrhizal fungus (Glomus mossea CA) and plant growth promoting rhizobacteria (PGPR) of Bacillus pantea and Psedomonas putida were evaluated on growth parameters of Osteospermum (Osteospermum hybrida ‘Passion Mix’) under different watering conditions (field capacity, two and one third of field capacity). Arbuscular mycorrhizal fungus (AMF) and PGPR were applied as single or mixed. A factorial trial based on randomized complete block design with 3 replications per treatment and 4 replicates was carried out under greenhouse conditions. Root colonization, growth parameters and P concentration in plant was measured. Results showed that PGPR and mycorrhizal inoculated plants had better nutritional conditions than that of non-inoculated plants. Moreover, there was a synergetic effect between AMF and PGPR in this study. PGPR were as much effective as AMF on alleviating the negative impacts of drought stress. According to the results, applied microorganisms were more effective on improving the growth parameters at 70% of field capacity, while at 40% of field capacity; their effective role was reduced, although the plants were persistent, yet. Results revealed exploitation of AMF and PGPR and administering an optimized irrigation regime can be effective on improving yield of this plant.

کلیدواژه‌ها [English]

  • Field capacity
  • Irrigation
  • microorganism
  • Root colonization
  • Symbiosis
Al-Karaki, G. N. (1998). Benefit, cost and water-use efficiency of arbuscular mycorrhizal durum wheat grown under drought stress. Mycorrhiza, 8(1), 41-45.
Al-Karaki, G. N. (2000). Growth of mycorrhizal tomato and mineral acquisition under salt stress. Mycorrhiza, 10(2), 51-54.
Aragno, M. (2003). Beneficial soil microorganisms: A key for sustainable agriculture. in Proceedingsof the Seventh International Conference on Kyusei Nature Farming.
Artursson, V. & Jansson, J.K. (2003). Use of bromodeoxyuridine immunocapture to identify active bacteria associated with arbuscular mycorrhizal hyphae. Applied and Environmental Microbiology, 69(10), 6208-6215.
Arshad, M. & Frankenberger Jr.W. (1989). Biosynthesis of ethylene byAcremonium falciforme. Soil Biology and Biochemistry, 21(5), 633-638.
Banerjee, M. R., Yesmin, L. & Vessey, J. K. (2006). Plant-growth-promoting rhizobacteria as biofertilizers and biopesticides. Handbook of microbial biofertilizers. Food Products Press, New York, p. 137-181.
Barazani, O. & Friedman, J. (1999). Is IAA the major root growth factor secreted from plant-growth-mediating bacteria? Journal of Chemical Ecology, 25(10), 2397-2406.
Bashan, Y. & Dubrovsky, J. (1996). Azospirillum spp. participation in dry matter partitioning in grasses at the whole plant level. Biology and Fertility of Soils, 23(4), 435-440.
Bianciotto, V., Minerdi, D., Perotto, S. & Bonfante P. (1996). Cellular interactions between arbuscular mycorrhizal fungi and rhizosphere bacteria. Protoplasma, 193(1-4), 123-131.
Bolan, N. (1991). A critical review on the role of mycorrhizal fungi in the uptake of phosphorus by plants. Plant and soil, 134(2), 189-207.
Burd, G. I., Dixon, D. G. & Glick, B. R. (1998). A plant growth-promoting bacterium that decreases nickel toxicity in seedlings. Applied and Environmental Microbiology, 64(10), 3663-3668.
Duponnois, R. & Garbaye, J. (1991). Effect of dual inoculation of Douglas fir with the ectomycorrhizal fungus Laccaria laccata and mycorrhization helper bacteria (MHB) in two bare-root forest nurseries. Plant and Soil, 138(2), 169-176.
Evans, M. L. & Cleland, R. E. (1985). The action of auxin on plant cell elongation. Critical reviews in plant sciences, 2(4), 317-365.
Frankenberger Jr, W. & Arshad, M. (1995). Phytohormones in soils: microbial production and function. Marcel Dekker Inc.
Frey-Klett, P., Pierrat, J.C. & Garbaye, J. (1997). Location and Survival of Mycorrhiza Helper Pseudomonas fluorescens during Establishment of Ectomycorrhizal Symbiosis between Laccaria bicolor and Douglas Fir. Applied and Environmental Microbiology, 63(1), 139-144.
Ghourchiani, M., Alikhani, H., Akbari, Gh., Zareie, M. & Dadi, I. (2012). Effect of phosphate solublizing bacteria, arbuscular mycorrhizal fungi and chemical P fertilizer on yield and yield characters of Zea mays under normal and water stress coditions in Karaj. Iranian Journal of Field Crops Research, 10(1), 214-224.
Giovannetti, M. & Mosse, B. (1980). An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection inroots. New phytologist, 84(3), 489-500.
Giovannini, A., Zottini, M., Morreale, G., Spena, A. & Allavena, A. (1999). Ornamental traits modification by rol genes in Osteospermum ecklonis transformed with Agrobacterium tumefaciens. In Vitro Cellular & Developmental Biology-Plant, 35(1), 70-75.
Graham, J. & Eissenstat, D. (1994). Host genotype and the formation and function of VAmycorrhizae. Plant and Soil, 159(1), 179-185.
Grichko,V. P. & Glick, B. R. (2001). Amelioration of flooding stress by ACC deaminase-containingplant growth-promoting bacteria. Plant Physiology and Biochemistry, 39(1), 11-17.
Goicoechea, N., Antolin, M., Strnad, M. & Sánchez-Díaz, M. (1996). Root cytokinins, acid phosphatase and nodule activity in drought-stressed mycorrhizal or nitrogen-fixing alfalfa plants. Journal of experimental botany, 47(5), 683-686.
Javed, I., Arshad, M. & Ali, K. (1998). Evaluation of rhizobacteria for their growth promoting activity in maize. Pakistan Journal of Soil Science, 2(1), 16-20.
Kapulnik, Y., Sarig, S., Nur, I., Okon, Y. & Henis, Y. (1982). The effect of Azospirillum inoculation on growth and yield of corn. Israel journal of botany, 31(1-4), 247-255.
Khandan‐Mirkohi, A. & Schenk, M.K. (2009). Phosphorus efficiency of ornamental plants in peat substrates. Journal of Plant Nutrition and Soil Science, 172(3), 369-377.
Khandan-Mirkohi, A., Taheri, M.R., Zafar-Farrokhi, F. & Rejali, F. (2015). The effect of mycorrhizal symbiosis on the water uptake efficiency and some growth traits of Osteospermum (Osteospermum hybrida ‘Passion Mix’). Iranian Journal of Horticultural Science, 45(4), 361-375.
Kohler, J., Caravaca, F., Carrasco, L. & Roldan, A. (2007). Interactions between a plant growth-promoting rhizobacterium, an AM fungus and a phosphate-solubilising fungus in the rhizosphere of Lactuca sativa. Applied Soil Ecology, 35(3), 480-487.
Koltai, H. (2010). Mycorrhiza in floriculture: difficulties and opportunities. Symbiosis, 52(2-3), 55-63.
Koske, R. & Gemma, J. (1989). A modified procedure for staining roots to detect VA mycorrhizas. Mycological research, 92(4), 486-488.
Marschner, H. & Dell, B. (1994). Nutrient uptake in mycorrhizal symbiosis. Plant and Soil, 159(1), 89-102.
Marulanda, A., Barea, J.-M. & Azcón, R. (2009). Stimulation of plant growth and drought tolerance by native microorganisms (AM fungi and bacteria) from dry environments: mechanisms related to bacterial effectiveness. Journal of plant growth regulation, 28(2), 115-124.
Meyer, J. R. & Linderman, R. (1986). Response of subterranean clover to dual inoculation with vesicular-arbuscular mycorrhizal fungi and a plant growth-promoting bacterium, Pseudomonas putida. Soil Biology and Biochemistry, 18(2), 185-190.
Molla, A., Shamsuddin, Z., Halimi, M., Morziah, M. & Puteh, A. (2001). Potential for enhancement of root growth and nodulation of soybean co-inoculated with Azospirillum and Bradyrhizobium in laboratory systems. Soil Biology and Biochemistry, 33(4), 457-463.
Nowak, J. (2001). The effects of rooting media, CO2 enrichment, P-nutrition and mycorrhizal inoculation on rooting and growth of Osteospermum. in International Symposium on Growing Media and Hydroponics, 644.
Nye, P.H. & Tinker, P.B. (1977). Solute movement in the soil-root system. Studies in Ecology. Blackwell Scientific Publications, Oxford. p. 342.
Pan, Y., Wu, L. J. & Yu, Z. L. (2006). Effect of salt and drought stress on antioxidant enzymes activities and SOD isoenzymes of liquorice (Glycyrrhiza uralensis Fisch). Plant growth regulation, 49(2-3), 157-165.
Patten, C. L. & Glick, B. R. (2002). Role of Pseudomonas putida indoleacetic acid in development of the host plant root system. Applied and Environmental Microbiology, 68(8), 3795-3801.
Pivonia, S., Levita, R., Dori, I., Ganot, L., Meir, S., Salim, S., Koltai, H. (2010). Application of mycorrhizae to ornamental horticultural crops: lisianthus (Eustoma grandiflorum) as a test case. Spanish Journal of Agricultural Research, 8(S1), 5-10.
Porcel, R. & Ruiz-Lozano, J. M. (2004). Arbuscular mycorrhizal influence on leaf water potential, solute accumulation, and oxidative stress in soybean plants subjected to drought stress. Journal of experimental botany, 55(403), 1743-1750.
Premachandra, G. S., Hahn, D. T., Rhodes, D. & Joly, R. J. (1995). Leaf water relations and solute accumulation in two grain sorghum lines exhibiting contrasting drought tolerance. Journal of experimental botany, 46(12), 1833-1841.
Rodríguez, H. & Fraga, R. (1999). Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology advances, 17(4), 319-339.
Sarig, S., Okon, Y. & Blum, A. (1992). Effect of Azospirillum brasilense inoculation on growth dynamics and hydraulic conductivity of Sorghum bicolor roots. Journal of Plant Nutrition, 15(6-7), 805-819.
Shaharoona, B., Arshad, M., Zahir, Z. A. & Khalid, A. (2006). Performance of Pseudomonas spp. containing ACC-deaminase for improving growth and yield of maize (Zea mays L.) in the presence of nitrogenous fertilizer. Soil Biology and Biochemistry, 38(9), 2971-2975.
Sohn, B. K., Kim, K. Y., Chung, S. J., Kim, W. S., Park, S. M., Kang, J. G., Lee, J. H. (2003). Effect of the different timing of AMF inoculation on plant growth and flower quality of chrysanthemum. Scientia horticulturae, 98(2), 173-183.
Srinath, J., Bagyaraj, D. & Satyanarayana, B. (2003). Enhanced growth and nutrition of micropropagated Ficus benjamina to Glomus mosseae co-inoculated with Trichoderma harzianum and Bacillus coagulans. World Journal of Microbiology and Biotechnology, 19(1), 69-72.
Tennant, D. (1975). A test of a modified line intersect method of estimating root length. The Journal of Ecology, 995-1001.
Tinker, P.B., Jones, M.D. & Durall, D.M. (1992). A functional comparison of ecto- and endo-mycorrhizas. In: D.J. Reid, D.H. Lewis, A.H. Fitter and I.J. Alexander (Ed), Mycorrhizas in Ecosystems. (pp. 303-310.) CAB International, Wellingford, England.
Toro, M., Azcón, R. & Barea, J. (1998). The use of isotopic dilution techniques to evaluate the interactive effects of Rhizobium genotype, mycorrhizal fungi, phosphate‐solubilizing rhizobacteria and rock phosphate on nitrogen and phosphorus acquisition by Medicago sativa. New phytologist, 138(2), 265-273.
Van Loon, L. (2007). Plant responses to plant growth-promoting rhizobacteria. European Journal of Plant Pathology, 119(3), 243-254.
Vessey, J.K. (2003). Plant growth promoting rhizobacteria as biofertilizers. Plant and soil, 255(2), 571-586.
Vivanco, J. M. & Flores, H. E. (2000). Control of root formation by plant growth regulators. Plant growth regulators in agriculture and horticulture. Ed., Basra, AS, Food Products Press, New York, 1-25.
Vosátka, M. & Gryndler, M. (1999). Treatment with culture fractions from Pseudomonas putida  modifies the development of Glomus fistulosum mycorrhiza and the response of potato and maize plants to inoculation. Applied Soil Ecology, 11(2), 245-251.
Zahir, Z. A., Arshad, M. & Frankenberger Jr, W. T. (2003). Plant growth promoting rhizobacteria: applications and perspectives in agriculture. Advances in Agronomy, 81, 97-168.