تأثیر فلز کادمیم بر توانائی زیستی و فعالیت برخی آنزیم‌های پاداکسندگی در پینة گیاه پریوش (Catharanthus roseus)

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکده علوم، دانشگاه اراک

2 استادیار، دانشکده علوم، دانشگاه اراک

چکیده

پریوش گیاهی زینتی و داروئی است که ازنظر اقتصادی نیز اهمیت دارد. از پینة (کالوس) گیاه پریوش دروایة (سوسپانسیون) یاخته‌ای تهیه و پس از تیمار با دزهای مختلف نیترات کادمیم به مدت 1، 3، 6 روز توانائی زیست توسط تریپان بلو و MTT بررسی شد. سوسپانسیون یاخته‌ای برای بررسی ریخت‌شناسی (مورفولوژی) و بافت پینه برای بررسی فعالیت آنزیم‌های کاتالاز، گایاکول پراکسیداز و سوپراکسید دیسموتاز و پراکسیداسیون لیپیدی پس از تیمار با 0، 20، 30 و 40 میلی‌مولار به مدت سه روز استفاده شد. داده‌ها به روش ANOVA یک‌سویه و آزمون دانکن تجزیه (آنالیز) و  p<0.05 به‌ عنوان سطح معنی‌داری در نظر گرفته شد. داده‌های تریپان بلو و MTT نشان‌دهندة تفاوت معنی‌دار (p<0.05) میانگین توانایی زیستی به‌صورت وابسته به دز در مقایسه با کنترل بود. همچنین فعالیت آنزیم‌‌های موردبررسی در پاسخ به تنش اکسایشی (اکسیداتیوی) ناشی از نیترات کادمیم افزایش معنی‌دار (p<0.05) نشان داد. میزان مالون دی آلدئید به‌عنوان محصول پراکسیداسیون لیپید نیز با افزایش معنی‌دار همراه بود. نیترات کادمیم باعث تخریب غشاء و کاهش توانائی زیستی یاخته‌‌ها شد، افزایش فعالیت آنزیم‌‌های پاداکسندگی قادر به جبران آسیب وارده نبود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effect of cadmium on viability and activity of some of the antioxidant enzymes in the callus of Catharanthus roseus

نویسندگان [English]

  • Hajar Moradipoor 1
  • Mohammad Hossein Abnosi 2
  • Mohammadreza Amirjani 2
  • Majid Mahdiyeh 2
1 Student
چکیده [English]

Catharantus-roseus is a medicinal and ornamental plant with growing attention toward its economical value. To determine cell viability based on trypan blue  and  MTT assay, cell suspension from callus of Catharantus-roseus were treated with different concentrations of cadmium-nitratee for 1, 3 and 6 days.  Also cell suspension was used to investigate the cell morphology for a period of 3 days using 0, 20, 30 and 40 mM of cadmium nitrate. In addition, the callus was also treated for a same period and concentrations to estimate the level of lipid peroxidation and the activity of superoxide dismutase, catalase and guaiacol peroxidase. Data was analysed using one-way-ANOVA (Duncan-test) and pCatharantus roseus. Activity of the antioxidant enzymes increased significantly but was not able to compensate the caused damages.

کلیدواژه‌ها [English]

  • Antioxidant Enzymes
  • cadmium nitrate
  • catharantus roseus callus
  • cellular response
  • Malondialdehyde
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