بررسی ریزازدیادی درون شیشه‌ای برخی از رقم‌های امیدبخش ’به‘ (Cydonia oblonga Mill.)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد گروه بیوتکنولوژی کشاورزی، دانشکده کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی واحد دامغان

2 دانشیار، بخش تحقیقات باغبانی، مؤسسة تحقیقات اصلاح و تهیة نهال و بذر، کرج

3 استادیار گروه بیوتکنولوژی کشاورزی، دانشکده کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی واحد دامغان

4 کارشناس ارشد بیوتکنولوژی کشاورزی، دانشکده کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، تهران

5 کارشناس ارشد بخش تحقیقات باغبانی، مؤسسه تحقیقات اصلاح و تهیه نهال و بذر، کرج

چکیده

در بین درختان میوة دانه‌دار، درخت ’به‘ حساس‌ترین میزبان‌ بیماری آتشک بوده و گزینش رقم‌های متحمل آن اهمیت دارد. در این تحقیق برای تعیین شرایط بهینة افزایش، ریز نمونه‌های رقم‌های امیدبخش KVD4، NB2، PH2 به همراه پایة کوئینس C و رقم شاهد اصفهان (KVD3) در محیط‌های MS، QL و QL تغییریافته (mQL)، ارزیابی و بیشترین میزان رشد در کمترین زمان و بهترین پرآوری در mQL مشاهده شد. در بررسی تأثیر غلظت­های مختلف دو هورمون گیاهی سایتوکینین‌ 2iP و BAP در محیط پایة mQL، به ترتیب برتری محیط حاوی 5/0 و 1 میلی‌گرم بر لیتر 2iP و BAP با بهترین شمار و کیفیت ریز شاخه‌ها مشخص شد. ریشه‌زایی شاخساره‌ها در تیمار دراز‌مدت با غلظت‌های 1/0 میلی‌گرم بر لیتر IBA و تأثیر توأم IBA وBAP با غلظت‌های‌ 1 میلی‌گرم بر لیتر و تیمار کوتاه‌مدت با غلظت‌های 5/0، 1 و 2 میلی‌گرم بر لیتر IBA، مشخص کرد که تیمار کوتاه‌مدت، ریشه‌زایی بهتری دارد. بیشترین درصد ریشه‌زایی، توسعة برگی، طول و کیفیت ریشه در غلظت 2 میلی‌گرم بر لیتر IBA در تیمار کوتاه‌مدت ایجاد شد. به‌طور‌کلی برای ریزازدیادی رقم‌ها و بقاء مطلوب ریز شاخه‌ها، محیط رشدی مشتمل بر نمک‌های پایة mQL غنی‌شده با 3 درصد ساکارز، 1 میلی‌گرم بر لیتر BAP، 1 میلی‌گرم بر لیتر 2iP، 5/0 درصد پکتین و 6/0 درصد آگار توصیه می‌شود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Study on in vitro propagation of some promising quince (Cydonia oblonga) cultivars

نویسندگان [English]

  • Fereshte Khosravinezhad 1
  • Hamid Abdollahi 2
  • Bahare Kashefi 3
  • Maryam Hassani 4
  • Zeinab Salehi 5
1 M.Sc Student, Agricultural Biotechnology Department, Faculty of Agriculture and Natural Resources, Islamic Azad University of Damghan Branch
2 Head of Tissue Culture lab and researcher of pome fruit trees
3 Assistant Professor, Agricultural Biotechnology Department, Faculty of Agriculture and Natural Resources, Islamic Azad University of Damghan Branch,
4 M.Sc, Agricultural Biotechnology Department, Faculty of Agriculture and Natural Resources, Islamic Azad University, Science and Research Branch, Tehran.
5 M.Sc, Horticulture Research Department, Seed and Plant Improvement Institute, Karaj
چکیده [English]

Among pome fruit trees, quince is the most susceptible tree to fire blight. Following selection of several promising quince cultivars, this research aimed at optimization of in vitro propagation protocol of the selected materials. Therefore, several experiments were carried out for determination of the best culture medium and plant growth regulators for establishment, proliferation and root induction of these cultivars. At first, proliferation and growth of KVD4, NB2 and PH2 genotypes were compared with Quince C and cv. Isfahan (KVD3) as control, in three culture media based on MS, QL and modified QL, in which mQL was observed to have the highest growth, and the best proliferation and shootlets quality. Then, mQL as the base culture medium was used to study effects of 2ip and BAP in two 0.5 and 1 mg/L concentrations. Based on the results, the culture medium containing 2ip (0.5 mg/L) and BAP (1 mg/L) showed the highest number and quality of shootlets. Finally, having examined root induction of the shoots in two long term treatments with IBA, 0.1 mg/L alone, and simultaneously with IBA and BAP (both 1 mg/L), and in short term treatment with IBA at 0.5, 1 and 2 mg/L concentrations, showed that the short term treatment gave better root induction. Also, the highest percentage of roots, leaf development, length and quality of roots resulted in short term treatment with 2 mg/L IBA.

کلیدواژه‌ها [English]

  • culture media
  • cytokinin
  • growth regulators
  • quince
  • root induction
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