تأثیر کیفیت نور و برخی تنظیم کننده‌های رشد بر شاخه‌زایی و ریشه‌زایی درون شیشه‌‌ای پایه گلابی OH×F333

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشگاه بوعلی سینا، همدان

2 دانشیار، دانشگاه بوعلی سینا، همدان

چکیده

این پژوهش به منظور بررسی تأثیر کیفیت نور بر ریزازدیادی پایه گلابی333OH × Fانجام شد. ابتدا ریزنمونه‌های تک گره روی محیط کشت QL تغییر یافته حاوی BA (0، 5/0، 1 و 2 میلی‌گرم در لیتر) و IAA (0، 1/0 و 1 میلی‌گرم در لیتر) کشت و از لحاظ شاخه‌‌زایی ارزیابی شدند. سپس گیاهچه‌‌‌ها در مناسب‌‌ترین ترکیب هورمونی کشت شده و در اتاقک رشد با چهار تیمار نوری فلورسنت (شاهد)، آبی، قرمز و ترکیب یکسان آبی و قرمز حاصل از LED با شدت 57 میکرومول در متر مربع در ثانیه قرار گرفتند. برای بررسی تأثیر نور بر ریشه‌زایی، ریزنمونه‌ها در محیط کشت QL تغییر یافته حاوی 1 میلی‌گرم در لیتر IAA کشت و در اتاقک رشد با چهار تیمار نوری مشابه شاخه‌زایی قرار گرفتند. بیشترین تعداد شاخساره در نور ترکیبی آبی و قرمز و بلندترین طول شاخساره و میانگره در نور قرمز مشاهده شد. همچنین بیشترین تعداد برگ و تعداد گره در تیمار نور آبی مشاهده شد. در مورد ریشه‌زایی نیز تیمارهای نوری تأثیر معنی‌داری بر درصد ریشه‌زایی، تعداد ریشه و طول ریشه نشان دادند. بیشترین درصد ریشه‌زایی با ریشه‌زایی تمامی شاخساره‌ها در تیمار ترکیبی آبی و قرمز مشاهده شد. در مقابل کمترین ریشه‌زایی، کمترین تعداد ریشه در گیاهچه و کمترین طول ریشه در تیمار نور آبی بدست آمد. با توجه به نتایج تیمار نور ترکیبی آبی و قرمز برای افزایش میزان شاخه‌‌زایی و ریشه‌زایی در این پایه نسبت به سایر تیمار‌های نوری مناسب‌تر بود و گیاهان تحت تأثیر نور ترکیبی تعداد شاخساره بیشتری را داشتند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Influence of light quality and some growth regulators on in vitro shoot proliferation and rooting of pear rootstock OH×F333

نویسندگان [English]

  • Seyede Maryam Mousavifattah 1
  • Hassan Sarikhani 2
1
2
چکیده [English]

This research was aimed to investgate the effect of light quality on micropropagation of pear rootstock OH×F333. First, single nodes explants were cultured in modified QL medium containing BA at four levels (0, 0.5, 1, and 2 mg/l-1) and IAA at three levels (0, 0.1, and 1 mg/l-1) and were evaluated for shoot proliferation. Then, plantlets subcultured in modified QL medium containing 1 mg/l-1 BA and were maintained in growth chamber under four level of light quality consisting red, blue and red-blue light provided by LED instrument and white cool light provided by florescent, all having 75 µmol m-2 s-1 intensity. For investigation of light quality on rooting, shoots were subcultured on modified QL medium containing 1 mg/l-1 IAA and were incubated under same light treatments. The results indicated that the highest number of proliferated shoots was obtained in red-blue light while the longest shoots and internodes was in red light treatment. Moreover, the number of leaves and nodes of plantlets grown under blue light were high. Light quality showed significant effect on rooting percentage, root number and root length. The highest rooting, with 100 percent rooting of shoots, was observed in red-blue light treatment. In contrary, the lowest percentage of rooting, the lowest number of root and the shortest length of root were observed in blue light treatment. Based on current results, combination of blue and red light in comparison to other light treatment was more appropriate treatment for shoot proliferation and rooting of pear rootstock OH×F333. The explants under this treatment showed higher number of shoots.

کلیدواژه‌ها [English]

  • OH × F333 pear rootstock
  • light quality
  • proliferation
  • LED
  • rooting

مراجع

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سابقه مقاله

  • تاریخ دریافت: 27 آذر 1392
  • تاریخ بازنگری: 10 مهر 1393
  • تاریخ پذیرش: 29 مهر 1393

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