تأثیر نوع پلی آمین بر انبارمانی میوه آلو، رقم ‘شابلون’

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناس ارشد، دانشکدۀ کشاورزی، دانشگاه تبریز

2 دانشیار، دانشکدۀ کشاورزی، دانشگاه تبریز

3 استادیار، دانشکدۀ کشاورزی، دانشگاه تبریز

چکیده

در این آزمایش تأثیر سه نوع پلی‌آمین پوتریسین، اسپرمیدین و اسپرمین بر عمر پس از برداشت میوۀ آلو رقم ‘شابلون’ بررسی شد. میوه ها در محلول پلی‌آمین‌ها با دو غلظت 1 و 2 میلی‌مولار و نیز آب مقطر (تیمار شاهد) به‌مدت 5 دقیقه غوطه‌ور و در سردخانه‌ای با دمای 5/0± 5/1 درجۀ سانتی‌گراد و رطوبت نسبی 80‌ـ 85 درصد انبار شدند. آزمایش در قالب فاکتوریل بر پایۀ طرح کاملاً تصادفی با 7 تیمار و 6 زمان انبارداری در سه تکرار انجام شد. پس از شروع انبارداری، هر هفته یک بار میزان سفتی بافت، اسیدیتۀ قابل تیتراسیون و pH میوه ها، مواد جامد محلول و کاهش وزن اندازه‌گیری شد. همچنین میزان تولید اتیلن در سه هفته انبارداری و میزان فعالیت آنزیم پلی‌گالاکتروناز در هفتۀ اول و آخر انبارمانی مطالعه شد. پلی‌آمین‌ها به‌طور معنا داری تولید اتیلن را نسبت به میوه های شاهد کاهش دادند و سبب حفظ سفتی میوه ها شدند. در بین پلی‌آمین‌ها اسپرمین 2 میلی‌مولار، مؤثرترین نوع پلی‌آمین در حفظ سفتی بافت بود. این تیمار به همراه پوتریسین 2 میلی‌مولار و اسپرمیدین 1 میلی‌مولار در جلوگیری از تولید اتیلن نیز مؤثر واقع شدند، در‌حالی‌که در جلوگیری از فعالیت آنزیم پلی‌گالاکتروناز، تقریباً بین سه نوع پلی‌آمین اختلاف معنا‌داری مشاهده نشد. تیمار پلی‌آمین‌ها به‌طور معنا داری از کاهش وزن میوه‌ها در طول دورۀ انبارداری جلوگیری کرد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of polyamines on storage life of plum fruit cv. ’Shablon ‘

نویسندگان [English]

  • Saba Shokrallah Fam 1
  • Jafar Hajiloo 2
  • Fariborz Zare Nahandi 3
1 Former M. Sc. Student, Faculty of Agriculture, University of Tabriz, Iran
2 Associate Professor, Faculty of Agriculture, University of Tabriz, Iran
3 Assistant Professor, Faculty of Agriculture, University of Tabriz, Iran
چکیده [English]

In this experiment, effects of three polyamines (putrescine, spermidine and spermine) on postharvest life of plum fruit cv. ’Shablonwere studied. Fruit was dipped in solution of polyamines with 1 and 2 mM and distilled water (control) for 5 min in a cold storage at 1.5±0.5°C and 80-85% RH. Experiment was conducted in a factorial with a complete randomized design with 7 treatments and 6 storage time with three replications. Fruit firmness, titratable acidity and pH, soluble solids and weight loss were evaluated once a week. Also, the rate of ethylene production was determined in three weeks, and polygalacturonase enzyme activity was measured at the first and last of storage life. Polyamines reduced ethylene production significantly and was maintained the fruit firmness. The most effective treatment in maintaining the firmness was 2 mM spermine. Also, this treatment was effective in preventing of ethylene production with 2 mM putrescine and 1 mM spermidine. No difference was found between the three polyamines in prevention of polygalacturonase enzyme activity. Polyamine treatments were prevented fruit weight loss significantly during storage.

کلیدواژه‌ها [English]

  • Ethylene
  • firmness
  • polygalacturonase
  • storage life
  • weight loss
  1. Ball, J.A. (1997). Evaluation of two lipid-based edible coatings for their ability to preserve postharvest quality of green bell peppers. Master Thesis. Faculty of the Virginia Polytechnic and state University, Blacksburg, Virginia, USA.
  2. Bors, N., Langebartels, C., Mitchel, C. & Sanderman, H. (1989). Polyamines as radical scavengers and protectants against ozone damage. Journal of Phytochemistry, 28, 1589-1595.
  3. Bradford, MM. (1976). A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principles of protein-dye binding. Journal of Annal Biochemical, 72, 248-254.
  4. Bregoli, A.M., Scaramagli, S., Costa, G., Sabatini, E., Ziosi, V., Biondi S. & Torrigiani, P. (2002). Peach (Prunus persica L.) fruit ripening: aminoethoxyvinylglycine (AVG) and exogenous polyamines affect ethylene emission and flesh firmness. Journal of Plant Physiology, 114, 472-481.
  5. Drolet, G., Dumbroff, E.B., Legge, R.L. & Thompson, J.E. (1986). Radical scavenging properties of polyamines. Journal of Phytochemistry, 25, 367-371.
  6. Heby, O. & Persson, L. (1990). Molecular genetics of polyamine synthesis in eukaryotic cells. Journal of Trends in Biochemical Sciences, 15, 153-158.
  7. Huber, DJ. (1983). The role of cell wall hydrolyses in fruit softening. Journal of Horticultural Review, 5, 169-219.
  8. Khan, A.S. Singh, Z., Abbasi, N.A. & Swinny, E.E. (2008). Pre or post-harvest application of putrescine and low temperature storage affect fruit ripening and quality of Agelino plum. Journal of Science of Food and Agriculture, 88, 1687-1695.
  9. Martinez-Romero, D., Serrano, M., Carbonell, A., Burgos, L., Riquelme F. & Valero. D. (2002). Effects of postharvest putrescine treatment on extending shelf life and reducing mechanical damage in apricot. Journal of Food Science, 67, 1706-1712.
  10. Martinez-Tellez, M.A., Ramos-Clamont, M.G., Gardea, A.A. & Vargas-Arispuro, I. (2002). Effect of infiltrated polyamines on polygalacturonase activity and chilling injury responses in Zucchini Squash (Cucurbita pepo L.). Journal of Biochemical and Biophysical Research Communications, 295(1), 98-101.
  11. Mirdehghan, S., Rahimi, M., Castillo, S., Martinez-Romero, D., Serrano, M. & Valero, D. (2007). Pre-storage application of polyamins by pressure or immersion improves shelf life of pomegranate stored at chilling temperature by increasing andogenous polyamine level. Journal of Post harvest Biology and Technology, 44(1), 26-33.
  12. Mostofi, Y. & Najafi, F. (2005). Laboratory analytical methods in horticultural science. Tehran University Publication. (In Farsi)
  13. Perez-Vicente, A., Martinez-Romero, D., Carbonell, A., Srrano, M., Riquelme, F., Guillen, F. & Valero, D. (2002). Role of polyamines in extending shelf life and reduction of mechanical damage during plum (Prunus Salicina Lind L.) storage. Journal of Postharvest Biology and Technology, 25(1), 25-32.
  14. Rahemi, M. (2005). Postharvest physiology: an introduction to physiology and handling of fruits, vegetables and ornamental plants (3rd ed.). Shiraz University Publication. (In Farsi)
  15. Serrano, M., Martinez-Romero, D. Guillen F. & Valero. D. (2003). Effects of exogenous putrescine on improving shelf life of four plum cultivar. Journal of Postharvest Biology and Technology, 30, 259-271.
  16. Sood, S. & Nagar, P.K. (2008). Post-harvest alteration in polyamins and ethylene in two diverse rose species. Journal of Acta Physiology Plant, 30, 243-248.
  17. Valero, D., Serrano, M., Martinea-Madrid, M.C. & Riquelme, F. (1997). Polyamines, Ethylen and physiochemical changes in low temperature-stored peach (Prunus Persica L. CV. Maycrest). Journal of Agriculture and Food Chemistery, 45, 3406-3410.
  18. Valero, D., Vicente, AP., Romero, DM., Castilo, S. & Guillen, FS. (2002). Plum storability improved after calcium and heat postharvest treatments. Journal of Food Science, 67, 2571-2575.
  19. Takahashi, T. & Kakehi, J.I. (2010). Polyamine: Ubiquitous polycations with unique roles in growth and stress responses. Journal of Annals of botany, 105, 1-6.
  20. Torrigiani, P., Bregoli, A. M., Ziosi, V., Scaramagli, S., Ciriaci, T., Rasori, A., Biondi, S. & Costa, G. (2004). Preharvest polyamine and AVG applications modulate fruit ripening in Stark Red Gold nectarines. Journal of Postharvest Biology and Technology, 33, 293-308.
  21. Zokaee Khosroshahi, M.R., Esna-Ashari, M., Ershadi, A. & Ahmadi. A. (2006). Effect of exogenous putrescine on post-harvest life of strawberry fruit, cultivar Selva. Journal of Agricultural research, soil, water and plant in agriculture, 6(1). (In Farsi).
  22. Zokaee Khosroshahi, M.R., Esna-Ashari, M. & Ershadi, A. (2007). Effect of exogenous putrescine on post-harvest life of strawberry (Fragaria ananassa Duch.) fruit, cultivar Selva. Journal of Scientia Horticulturae, 114, 27-32.
  23. Zokaee, Khosroshahi, M.R. & Esna-Ashari, M. (2008). Effect of putrescine on shelflife and postharvest physiology of strawberry, apricot, peach and cherry. Journal of Science and Technology of Agriculture and Natural Resources, 12(45). (In Farsi).
  24. Zokaee, Khosroshahi, M.R. & Esna-Ashari., M. (2008). Effect of exogenous putrescine treatment on the quality and storage life of peach (Prunus persica L.) fruit. Journal of Postharvest Technology and Innovation, 1, 278-287.