نگهداری بذر پده (Populus euphratica Oliv.) در شرایط فراسرد (Cryopreservation) و ارزیابی بذور فراسردی در آزمایشگاه و گلخانه

نوع مقاله : مقاله پژوهشی

نویسندگان

1 . کارشناس ارشد پژوهشی، استادیار پژوهش و استاد پژوهش، مؤسسۀ تحقیقات جنگل‏ها و مراتع کشور

2 . M.Sc. Student, Assistant Professor and Professor, Research Institute of Forest and Rangeland, Tehran, Iran

3 کارشناس، ادارۀ منابع طبیعی شهرستان املش، استان گیلان

4 کارشناس ارشد پژوهشی، استادیار پژوهش و استاد پژوهش، مؤسسۀ تحقیقات جنگل‏ها و مراتع کشور

چکیده

پده (Populus euphratica Oliv.) گونۀ بومی مقاوم به گرما، سرما و شوری است و دامنۀ اکولوژیک گسترده‏ای دارد. ماندگاری بذر پده در شرایط متعارف بسیار ‌کوتاه است و امکان نگهداری آن در شرایط بانک ژن وجود ندارد. برای نگهداری بذر این گونه در شرایط عادی (‏°C22+) و فراسرد یا ازت مایع (°C196-) از سه تیمار PVS2، گلیسرول 30 درصد و آبگیری همراه با بذور شاهد استفاده شد. بذور به‌مدت یک‏ هفته، یک ماه و یک سال در شرایط فراسرد ذخیره و در ‏°C22+ به ژرمیناتور وارد شدند. جوانه‏زنی بذور در ‏°C22+ در پیش‏تیمارهای گلیسرول 30 درصد و PVS2، از 90 درصد به 00/35 و 67/41 کاهش یافت. جوانه‏زنی در یک سال ذخیره‏سازی، در شرایط فراسرد (°C196-) از 90 در‌صد به 67/26 و 33/28 کاهش یافت. تیمار کاهش رطوبت بذر یا آبگیری بهترین نتیجه را در جوانه‏زنی و استقرار بذر در آزمایشگاه و گلخانه داشت. بذور شاهد در شرایط عادی بیش از چند ماه زنده‏مانی نداشتند و بذور فراسردی نیز در طول یک سال ماندگاری در شرایط فراسرد (°C196-) تغییری در زنده‏مانی، جوانه‏زنی و استقرار آن‌ها مشاهده نشد.

کلیدواژه‌ها

عنوان مقاله [English]

Cryopreservation of Populus euphratica Oliv. Seeds and Evaluation of the Cryopreserved Seeds under Laboratory and Greenhouse Conditions

نویسندگان [English]

  • Maryam Jebeli 1
  • Mohabat Ali Naderi Shahab 2
  • Hamid Reza Feizi 3
  • Alh Ashraf Jafari 4
1 . M.Sc. Student, Assistant Professor and Professor, Research Institute of Forest and Rangeland, Tehran, Iran
2 . M.Sc. Student, Assistant Professor and Professor, Research Institute of Forest and Rangeland, Tehran, Iran
3 B.Sc., Agriculture and Natural Resources Research Center, Amlash, Gilan
4 . M.Sc. Student, Assistant Professor and Professor, Research Institute of Forest and Rangeland, Tehran, Iran
چکیده [English]

Populus euphratica Oliv. is a highly heat, saline and cold tolerant native species, with wide range of ecological habitats and conditions. Seed longevity of P. euphratica is too short to be stored in traditional gene banks. For evaluating preservation of P. euphratica seeds in normal conditions (+22oC) as well as in cryogenic conditions (-196oC), three pre-cryopreservation treatments including PVS2, Desiccation and 30% Glycerol were applied before transferring the seeds into liquid nitrogen (-196oC) for 1 week, 1 month and 1 year. To evaluate the effects of the pre-treatments on seed germination and establishment, a part of the seeds were treated with PVS2, Desiccation and 30%Glycerol and incubated at +22oC, instead of liquid nitrogen (-196oC). The 30%Glycerol and PVS2 pre-treatments dramatically reduced the seed germination from 90% in control seeds to 35.00 and 41.67%. Cryogenic (-196oC) condition reduced seed germination from 90% in control seeds to 26.67% and 28.33. However, Desication showed excellent protection of seeds in cryogenic conditions in seed germination and establishment in lab and greenhouse. Control seed in normal conditions stayed for few months. Germination of cryopreserved seeds for a period of one year did not show significant reduction, compared to those kept for one week or one month at -196 oC.

کلیدواژه‌ها [English]

  • seed
  • Populus euphratica
  • Cryopreservation
  • desiccation
  • liquid nitrogen

مراجع

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سابقه مقاله

  • تاریخ دریافت: 27 اسفند 1392
  • تاریخ بازنگری: 09 تیر 1393
  • تاریخ پذیرش: 21 تیر 1393

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