نگهداری بذر پده (Populus euphratica Oliv.) در شرایط فراسرد (Cryopreservation) و ارزیابی بذور فراسردی در آزمایشگاه و گلخانه

نوع مقاله : مقاله پژوهشی

نویسندگان

1 . کارشناس ارشد پژوهشی، استادیار پژوهش و استاد پژوهش، مؤسسۀ تحقیقات جنگل‏ها و مراتع کشور

2 . M.Sc. Student, Assistant Professor and Professor, Research Institute of Forest and Rangeland, Tehran, Iran

3 کارشناس، ادارۀ منابع طبیعی شهرستان املش، استان گیلان

4 کارشناس ارشد پژوهشی، استادیار پژوهش و استاد پژوهش، مؤسسۀ تحقیقات جنگل‏ها و مراتع کشور

چکیده

پده (Populus euphratica Oliv.) گونۀ بومی مقاوم به گرما، سرما و شوری است و دامنۀ اکولوژیک گسترده‏ای دارد. ماندگاری بذر پده در شرایط متعارف بسیار ‌کوتاه است و امکان نگهداری آن در شرایط بانک ژن وجود ندارد. برای نگهداری بذر این گونه در شرایط عادی (‏°C22+) و فراسرد یا ازت مایع (°C196-) از سه تیمار PVS2، گلیسرول 30 درصد و آبگیری همراه با بذور شاهد استفاده شد. بذور به‌مدت یک‏ هفته، یک ماه و یک سال در شرایط فراسرد ذخیره و در ‏°C22+ به ژرمیناتور وارد شدند. جوانه‏زنی بذور در ‏°C22+ در پیش‏تیمارهای گلیسرول 30 درصد و PVS2، از 90 درصد به 00/35 و 67/41 کاهش یافت. جوانه‏زنی در یک سال ذخیره‏سازی، در شرایط فراسرد (°C196-) از 90 در‌صد به 67/26 و 33/28 کاهش یافت. تیمار کاهش رطوبت بذر یا آبگیری بهترین نتیجه را در جوانه‏زنی و استقرار بذر در آزمایشگاه و گلخانه داشت. بذور شاهد در شرایط عادی بیش از چند ماه زنده‏مانی نداشتند و بذور فراسردی نیز در طول یک سال ماندگاری در شرایط فراسرد (°C196-) تغییری در زنده‏مانی، جوانه‏زنی و استقرار آن‌ها مشاهده نشد.

کلیدواژه‌ها


عنوان مقاله [English]

Cryopreservation of Populus euphratica Oliv. Seeds and Evaluation of the Cryopreserved Seeds under Laboratory and Greenhouse Conditions

نویسندگان [English]

  • Maryam Jebeli 1
  • Mohabat Ali Naderi Shahab 2
  • Hamid Reza Feizi 3
  • Alh Ashraf Jafari 4
1 . M.Sc. Student, Assistant Professor and Professor, Research Institute of Forest and Rangeland, Tehran, Iran
2 . M.Sc. Student, Assistant Professor and Professor, Research Institute of Forest and Rangeland, Tehran, Iran
3 B.Sc., Agriculture and Natural Resources Research Center, Amlash, Gilan
4 . M.Sc. Student, Assistant Professor and Professor, Research Institute of Forest and Rangeland, Tehran, Iran
چکیده [English]

Populus euphratica Oliv. is a highly heat, saline and cold tolerant native species, with wide range of ecological habitats and conditions. Seed longevity of P. euphratica is too short to be stored in traditional gene banks. For evaluating preservation of P. euphratica seeds in normal conditions (+22oC) as well as in cryogenic conditions (-196oC), three pre-cryopreservation treatments including PVS2, Desiccation and 30% Glycerol were applied before transferring the seeds into liquid nitrogen (-196oC) for 1 week, 1 month and 1 year. To evaluate the effects of the pre-treatments on seed germination and establishment, a part of the seeds were treated with PVS2, Desiccation and 30%Glycerol and incubated at +22oC, instead of liquid nitrogen (-196oC). The 30%Glycerol and PVS2 pre-treatments dramatically reduced the seed germination from 90% in control seeds to 35.00 and 41.67%. Cryogenic (-196oC) condition reduced seed germination from 90% in control seeds to 26.67% and 28.33. However, Desication showed excellent protection of seeds in cryogenic conditions in seed germination and establishment in lab and greenhouse. Control seed in normal conditions stayed for few months. Germination of cryopreserved seeds for a period of one year did not show significant reduction, compared to those kept for one week or one month at -196 oC.

کلیدواژه‌ها [English]

  • seed
  • Populus euphratica
  • Cryopreservation
  • desiccation
  • liquid nitrogen
 
Blakesley, D. & Kiernan, R.J. (2001). Cryopreservation of axillary buds of a Eucalyptus grandis X Eucalyptus camaldulensis hybrid. CryoLetters, 22 (1), 13-18.
Calagari, M., Jafari Mofidabadi, A., Tabari, M. & Hoseini, S.M. (2007). Genetic variation on natural population of Populus euphratica Oliv. by peroxidase isoenzyme. Iranian Journal of Forest and Poplar Research, 15(2), 115-122.
Calagari, M., Jafari Mofidabadi, A., Tabari, M. & Hosseini, S.M. (2004). Intraspecific hybridization of Populus euphratica Oliv. using in vitro technique. Journal of Science, 15(2), 109-112.
Cho, E.G., Hor, Y.L., Kim, H. H., Rao, V.R. & Engelmann, F. (2002a). Cryopreservation of Citrus madurensis embryonic axes by encapsulation- dehydration. CryoLetters, 23(5), 325-332.
Cho, E.G., Hor, Y.L., Kim, H.H., Rao, V.R. & Engelmann, F. (2002b). Cryopreservation of Citrus madurensis embryonic axes by vitrification: importance of loading and treatment with vitrification soulution. CryoLetters, 23(5), 317-324.
Jafari, M.A. & Klagary, M. (2004). Inter and intra specific hybridization in Populus euphratica Oliv. Through ovary and ovule culture. Proceeding of the Fourth International Iran & Russia Conference, pp. 159-163.
Jitsopakul, N., Tammasiri, K. & Ishikawa, K. (2008). Cryopreservation of Bletilla striata seeds, 3-day germinating seeds and protocorms by droplet-vitrification. CryoLetters, 29(6), 517-526.
Lambardi, M., Fabbri, A. & Caccavale, A. (2000). Cryopreservation of white poplar (Populus alba L.) by vitrification of in vitro – grown shoot tips. Plant Cell Reports, 19(3), 213-218.
Matsumoto, T., Sakai, A. & Yamada, K. (1994). Cryopreservation of in vitro-grown apical meristems of wasabi (Wasabia japonica) by vitrification and subsequent high plant. Plant Cell Report, 13, 442-446.
Kuleshova L.L., MacFarlane D.R., Trounson A.O. & Shaw J.M. (1999). Sugars exert a major influence on the vitrification properties of ethylene glycol–based solutions and have low toxicity to embryos and oocytes. Cryobiology, 38(2), 119-130.
Mofidabadi, A.J., Jourabchi, A., Shahrzad, S. & Mahmodi, F. (2001). New genotypes development of Populus euphratica Oliv. Using gametoclonal variation. Silvae Genetica, 50(5-6), 257-279.
Mofidabadi, A.J., Modir-Rahmati, A.R. & Tavesoli, A. (1998). Application of ovary and ovule culture in Populus alba L. x P. euphratica Oliv. hybridization. Silvae Genetica, 47(5-6), 332-334.
Naderi Shahab,M., Hatami,F., Tabari, M. & Jafari, A.A. (2009). Cryopreservation and evaluation of chinese arbor-vitae (Biota orientalis) Seeds. Journal of New Seeds, 10(4), 264-276.
Sabeti, H. (2006). Forests trees and shrubs of Iran.(4th ed.) Publication of Yazd University. pp: 536.
Saito, Y., Shiraishi, S., Tanimoto, T., Yin, L., Watanabe, S. & Ide, Y. (2002). Genetic diversity of Populus euphratica populations in northwestern China determined by RAPD DNA analysis. New Forests, 23(2), 97-103.
Sakai, A., Kobayashi, S. & Oiyama, I. (1991). Survival by vitrification of nucellar cells of navel orange (Citrus sinensis Osb.var. brasiliensis Tanaka) cooled to –196°C. Journal of Plant Physiology, 137, 465-470.
Soleimani, A., Etemad, V., Kalagari, M., Namiranian, M. & Shirvani, A. (2011). Germination percentage of Populus euphratica Oliv. originated from different provenances and comparing seedling production in greenhouse and in vitro cultures. Journal of Forest and Wood Products (JFWP), Iranian Journal of Natural Resources, 63(4), 387-397.
Walters, C., Touchell, D.H., Power, P., Wesley-Smith, J. & Antolin, M.F. (2002). A cryopreservation protocol for embryos of the endangered species Zizania texana. CryoLetters, 23, 291-298.
Walters, C., Wheeler, L.J. & Stanwood, P.C. (2004). Longevity of cryogenically-stored seeds. Cryobiology, 48, 229-244.
Wang, Z.H. & Deng, X.X. (2004). Cryopreservation of shoot-tips of citrus using vitrification: effect of reduced form of glutathione. CryoLetters, 25, 43-50.
Wen, B. & Song, S. (2007). Acquisition and loss of cryotolerance in livistona chinensis embryos during seed development. CryoLetters, 28(4), 291-302.
Volk, G.M., Harris, J.L. & Rotindo, K.E. (2006). Survival of mint shoot tips after exposure to cryoprotectant solution components. Cryobiology, 52, 305-308.
González-Benito M.E., Aguilar N. and Avila T. 2009. Germination and embryo rescue from Passiflora species seeds post-cryopreservation. CryoLetters 30 (2): 142-147.
Wood C.B., Pritchard H.W. and Lindegaard K. 2003. Seed cryopreservation and longevity of tow Salix hybrids. CryoLetters 24: 17-26.