اثر اسپرمیدین بر ماده‏زایی شش توده پیاز خوراکی بومی استان خراسان در محیط درون‌شیشه‏ ای

نوع مقاله : مقاله پژوهشی

نویسندگان

دانشجوی سابق کارشناسی ارشد، دانشیار و استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

چکیده

این پژوهش به‌‌منظور بررسی اثر دو غلظت اسپرمیدین (5/0 و 1 میکرو‏مولار) بر القای ماده‏زایی شش توده پیاز خوراکی بومی استان خراسان در محیط درون‌شیشه‏ای انجام شد. نتایج نشان داد بیشترین و کمترین درصد رویان‏زایی به‌ترتیب در محیط‏های کشت M2 و M5 به دست آمد. بیشترین (41/1) و کمترین (33/0) درصد رویان‏زایی به‌ترتیب متعلق به توده‏های روشناوند بیرجند و سفید نیشابور بود. بیشترین درصد باززایی (33/3) مربوط به تودۀ آشخانۀ بجنورد در محیط کشت M2 بود. تودۀ آشخانۀ بجنورد و تودۀ درگز بیشترین و کمترین درصد بقای گیاه را داشتند (‌به‌ترتیب 25/81 و80/49 درصد). بنابراین، اسپرمیدین در دو غلظت 5/0 و 1 میکرومولار، بدون تنظیم‌کننده‏های رشد گیاهی دیگر، بر ماده‏زایی پیاز تأثیر مثبت نداشت و نبایستی جایگزین 2,4-D و BA شود. ترکیب اسپرمیدین با هورمون‌های دیگر رشدی می‏تواند سبب القای ماده‏زایی در پیاز شود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of spermidine on in vitro gynogenesis of six onion accessions from Khorasan province

نویسندگان [English]

  • Zhila Zanghue
  • Mohamad Reza Hasandokht
  • Abd-o-lkarim Kashi
. Former Graduate Student, Associate Professor and Professor, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

In order to investigate the effect of two spermidine concentrations (0.5 and 1 µ M ) on in vitro gynogenesis of six onion accessions from Khorasan province this research was carried out. Results showed that the highest and lowest percentage of gynogenesis rate was belonged to M2 and M5 media, respectively. The highest (1.41) and lowest (0.33) percentage of gynogenic embryo were belonged to Roshnavand-e- Birjand and Sefid-e- Naishabur accessions, respectively. The highest percentage of plant regeneration was belonged to Roshnavand-e- Birjand accession in M2 medium culture. Ashkhane-e- Bojnourd and Dargaz accessions had the highest and lowest percentage of plant survival rate (81.25 and 49.8, respectively). Therefore, spermidine in two concentrations of 0.5 and 1 µ M, without other plant growth regulators, had no positive effect on onion gynogenesis and should not be used instead of 2,4-D and BA. Combination of spermidine with other plant hormones could induce gynogenesis in onion.

کلیدواژه‌ها [English]

  • Onion
  • gynogenesis
  • In vitro culture
  • Spermidine
  1.  

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