بررسی اثر محلول‌پاشی پوترسین بر افزایش تحمل دانهال‌های گردوی ایرانی(Juglans regia L.) به تنش خشکی

نوع مقاله : مقاله پژوهشی


دانشجوی سابق کارشناسی ارشد و استادیار، دانشگاه شهید باهنر کرمان، دانشکدۀ کشاورزی


به‌منظور بررسی اثر محلول‏پاشی غلظت‏های مختلف پوترسین بر افزایش تحمل دانهال‏های گردوی ایرانی به تنش خشکی، آزمایشی به‌صورت فاکتوریل در قالب طرح پایۀ کاملاً تصادفی با 9 تیمار و 20 تکرار در یک گلخانۀ کاملاً کنترل‌شده اجرا شد. تیمارها شامل پوترسین در غلظت‏های صفر، 5/0 و 1 میلی‏مولار و تنش خشکی در سه سطح شاهد (80‌درصد ظرفیت زراعی)، تنش متوسط (50‌درصد ظرفیت زراعی) و تنش شدید (20‌درصد ظرفیت زراعی) بودند. نتایج مربوط به پارامترهای مورفولوژیکی، فیزیولوژیکی و بیوشیمیایی نشان داد که محلول‏پاشی دانهال‌ها با غلظت 1‏‏ میلی‏مولار پوترسین با تأثیر بر پارامترهای فیزیولوژیکی و بیوشیمیایی و بهبود فعالیت آنزیم‏های آنتی‌اکسیدانی (افزایش آنزیم‏های کاتالاز، آسکوربات پراکسیداز و گایاکول پراکسیداز) سبب افزایش تحمل دانهال‏های گردوی ایرانی به تنش خشکی می‌‌شود. بیشترین میزان طول ساقه (67/35 سانتی‌متر)، سطح برگ (6/303 سانتی‌متر مربع)، محتوای نسبی آب برگ (51/58‌درصد)، وزن تر کل (20/47 گرم) و کمترین نشت یونی (28/28‌درصد) مربوط به تیمار 1 میلی‌مولار پوترسین است. مشخص شد که محلول‌پاشی پوترسین با غلظت 1 میلی‌مولار می‌تواند در افزایش تحمل دانهال‌های گردوی ایرانی (Juglans regia L.) به تنش خشکی مؤثر است.


عنوان مقاله [English]

Effect of foliar application of putrescine to enhance drought tolerance of Persian walnut (Juglans regia L.) seedlings

نویسندگان [English]

  • Parisa Parvin
  • Masood Khezri
Former Graduate Student and Assistant Professor, Shahid Bahonar University of Kerman, Kerman, Iran
چکیده [English]

To study the effect of foliar application of putrescine on enhanceing drought tolerance of Persian walnut (Juglans regia L.) seedlings, an experiment had been carried out as factorial based on completely randomized design in a controlled greenhouse with 9 treatments and 20 replications. In this study, treatments were putrescine (0, 0.5 and 1 mM) and three stress levels including control (80% of field capacity), moderate drought stress (50% field capacity) and severe drought stress (20% field capacity). The results of morphological, physiological and biochemical parameters showed that these parameters were affected by the stress levels as well as different concentrations of putrescine. Foliar spray of 1mM putrescine affected the physiological and biochemical parameters and enhanced the tolerance of Persian walnut seedlings to drought stress through improving antioxidant activity systems (via increasing catalase, ascorbate peroxidase and guaiacol peroxidase). The highest rate of shoot length (35.67 cm), leaf area (303.6 cm), leaf relative water content (58.51%), the total fresh weight (47.20 mg) and the lowest ion leakage (28.28%) was related to 1 mM putrescine treatment. It was found that foliar spray of 1mM putrescine was effective on enhanceing drought tolerance of Persian walnut (Juglans regia L.) seedlings.

کلیدواژه‌ها [English]

  • Field capacity
  • polyamines
  • Antioxidant Enzymes
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