معرفی پروتکل تجاری به منظور ازدیاد درون شیشه‌ای کلم زینتی Brassica oleracea var. acephala

نوع مقاله: مقاله کامل

نویسندگان

استادیار دانشکده کشاورزی ومنابع طبیعی دانشگاه اراک

چکیده

طی سالیان اخیر کلم زینتی به‌دلیل داشتن اندام‏های هوایی رنگی بادوام و مقاومت خوب به شرایط محیطی در فضای سبز بسیار استفاده می‌شود. بنابراین، ازدیاد درون‌شیشه‏ای کلم زینتی در چند آزمایش مطالعه شد. غلظت‏های مختلف بنزیل آدنین (mg/L1/0، 5/0 و 1)، 2,4-D (mg/L1/0، 5/0)، نیترات نقره (mg/L 2و4) و نوع رقم (بنفش و سفید) در مرحلۀ استقرار به‏کار گرفته شد. همچنین تیمارهای مرحلۀ پرآوری شامل بنزیل آدنین (mg/L1/0 و 5/0) و ایندول بوتیریک اسید (mg/L1/0 و 5/0) بود. سازگاری گیاهچه‏های درون‌شیشه‏ای در مرحلۀ سازگاری با استفاده از غلظت‏های مختلف MS و عصارۀ ورمی کمپوست (کامل و یک دوم) اجرا شد. نتایج نشان داد کاربرد  mg/L1/0 بنزیل آدنین به همراه mg/ L 1 2,4-D باززایی قابل قبولی را در این گیاه زینتی فراهم می‌آورد. محیط کشت پرآوری تکمیل‌شده با mg/L 5/0 از بنزیل آدنین و ایندول بوتیریک اسید سبب توسعۀ شاخساره‏ها شد. سرانجام گیاهچه‏های درون‌شیشه‌ای با انتقال به محیط کشت MS تقلیل‌یافته به‌طور موفقیت‌آمیزی سازگار شدند. به‌طور‌کلی، در این پژوهش با استفاده از محیط کشت نسبتاً کم‌هزینه، در دسترس و حداقل غلظت تنظیم‌کننده‌های باززایی موفقیت‌آمیز کلم زینتی به دست آمد.

کلیدواژه‌ها


عنوان مقاله [English]

Introduction of commercial protocol for in vitro proliferation of Brassica oleracea var. acephala

نویسندگان [English]

  • Mina Taghizadeh
  • Mousa Solgi
چکیده [English]

In recent years, ornamental kale is commonly used in landscapes due to its colorful shoots and resistance to the diverce environmental conditions. Thus, its in vitro proliferation was studied in series of experiments. Different BA (0/10.1, 0.5 and 1 mg/L), 2,4-D (0.1, 0.5 mg/L) and silver nitrate (2, 4 mg/L) treatments were applied for the establishment stage. Treatments of the proliferation stage also included BA (0.1 and 0.5 mg/L) and IBA (0.1 and 0.5 mg/L). Acclimatization of in vitro plantlets was conducted using different concentrations of MS (complete and half strength) and vermicompost extract (direct and diluted). Results revealed that, using of 0.1 mg/L BA with one mg/L 2,4-D was able to provide regeneration in this ornamental plant, acceptably. Proliferation medium supplemented with 0.5 and 0.5 mg/L BA caused shoot development. Finally, in vitro plantlets were successfully adapted by transferring to media containing reduced MS medium without plant growth regulators. In conclusion, by using the relatively low cost, accessible media and the least concentrations of plant growth regulators, successful regeneration of kale ornamental were obtained.

کلیدواژه‌ها [English]

  • Kale ornamental
  • in vitro regeneration
  • plant growth regulators
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