تاثیر ریزموجودات مفید روی پاسخ‌های مورفوفیزیولوژیکی انار رقم شیشه‌کپ تحت تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشددانشکده کشاورزی دانشگاه فردوسی مشهد

2 استاد دانشکده کشاورزی دانشگاه فردوسی مشهد

3 مربی پژوهشی مرکز تحقیقات انار دانشگاه فردوسی مشهد

چکیده

ریزموجودات مفید (EM)، یکی از کود‏های زیستی تجاری شامل باکتری‏های فتوسنتزی، باکتری‏های اسید‏ لاکتیک، قارچ و اکتینومیست‏هاست. برای ارزیابی اثر ریزموجودات مفید (صفر و 1 درصد) و تنش شوری (0، 50، 100 و 150 میلی‏مولار) بر صفات مورفوفیزیولوژیک انار رقم شیشه‏کپ، آزمایشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با چهار تکرار در گلخانۀ تحقیقاتی دانشگاه فردوسی مشهد انجام گرفت. برای اعمال تنش شوری از کلرید کلسیم و کلرید سدیم به نسبت 1:10 استفاده شد. شاخص‏های مطالعه‌شده شامل ارتفاع ساقۀ اصلی، وزن تر اندام هوایی، طول ریشه، وزن خشک ریشه و حجم ریشه، محتوی نسبی آب برگ، شاخص کلروفیل و نشت الکترولیت، محتوی پتاسیم و سدیم بودند. نتایج نشان داد که تمامی شاخص‏های مورفوفیزیولوژیک اندازه‏گیری‌شده به‌جز نشت الکترولیت در تیمار 1‌درصد EM به‌طور معنا‏داری بیشتر از تیمار بدون EM تحت شرایط شوری بود. همچنین تحت شرایط تنش شدید شوری (150 میلی‏مولار) استفاده از EM موجب کاهش 5/12‌درصدی سدیم و افزایش 5/11‌درصدی پتاسیم، در مقایسه با عدم کاربرد EM شد. بر‌اساس نتایج حاصل از این پژوهش، به نظر می‏رسد، کاربرد ریزموجودات مفید می‏تواند اثرات مضر شوری را در سطوح پایین تنش کاهش ‏دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of ‘Effective Microorganisms’ on morpho-physiological traits in commercial cultivar of pomegranate (Punica granatum L. cv. shishe-kap) under salinity

نویسندگان [English]

  • Mohammad aref Poorian 1
  • Golam hosein Davari nejad 2
  • Yahya Selahvarzi 3
چکیده [English]

Effective microorganisms (EM) is a commercial biofertilizer mainly consists of photosynthetic and lactic acid bacteria, yeast and actinomycetes. In order to evaluate the influence of EM (0 and %1) and salt stress (0 mM, 50 mM, 100 mM and 150 mM) on the morphophysiological characteristics of pomegranate cv. shishe-kap, a factorial experiment was conducted based on the completely randomized design at the research greenhouse of Ferdowsi University of Mashhad. NaCl (10 part) and CaCl2 (1 part) were utilized to exert the salinity stress. Studied parameters included the main stem height, shoot fresh weight, root length, root dry weight, root volume, leaf relative water content, electrolyte leakage, chlorophyll index, potassium and sodium contents. The results showed that all the measured morphophysiological parameters expect the electrolyte leakage were significantly higher in EM treatment in comparison to no EM treatmant in saline conditions. In addition, under the extreme salinity stress (150 mM), application of 1% EM decreased the sodium content of to 12.5% and increased the potassium content to 11.5%. According to the obtained results from the above parameters, it seems that EM can reduce the harmful effects of salinity.

کلیدواژه‌ها [English]

  • Effective microorganisms
  • leaf relative water content
  • stem height
  • Chlorophyll index
  1. Allahverdiyev, S. R., Atik, A., Bayazıt, S. I. & Aida, S. (2011). The response of photosystem II and photosynthetic pigments to salt and Baikal I in tree seedlings. African Journal of Biotechnology, 10(4), 535-538.
  2. Bhatia, S.C. (1991). Biochemistry in Agricultural Sciences Vol. II. Shree Publishers & Distributors. New Delhi, India.
  3. Blum, A. & Ebercon, A. (1981). Cell membrane stability as a measure of drought and heat tolerance in wheat. Crop Science, 21, 43-47.
  4. Daly, M.J. & Stewart, D.P.C. (1999). Influence of effective microorganisms (EM) on vegetable production and carbon mineralization—a preliminary investigation. Journal of Sustainable Agriculture, 14, 15–25.
  5. Garg, G. (2010). Response in germination and seedling growth in Phaseoulus mungo under salt and drought stress. Journal of Environmental Biology, 31, 261-264.
  6. German, M. A., Burdman, S. & Okon, Y. ( 2000). Effects of AzosPirillum brasilense on root morphology of common bean under different water regims. Biology and Fertility of Soils, 32, 259 – 264.
  7. Grattan, S. R. & Grieve, C.M. (1999). Salinity – mineral nutrient relations in horticultural crops. Scientia Horticulturae, 78, 127-157.
  8. Heidari Sharif Abad, H. (2001). Plants and salinity. Research Institute of Forests and Rangelands press. Tehran. (In Farsi).
  9. Higa, T. (1993). An Earth Saving Revolution. Sunmark Publishing Tokyo, Japan.
  10. Higa, T. (1991). Effective microorganisms: a biotechnology for mankind. pp. 8–14. In: J.F. Parr., S.B. Hornick., C.E. Whitman (Eds.) Proceedings of the First International Conference on Kyusei Nature Farming. U.S. Department of Agriculture, Washington, DC, USA.
  11. Imai, S. & Higa, T. (1994). Kyusei nature farming in japan: Effect of EM on the growth and yield of spinach. P. 92-96. In parr., J.F. S.B. Hornick and M.E. Simpson (ed). Proceedings of the Second International Conference on Kyusei Nature Farming. U.S.Department of Agriculture, Washington, D.C., USA.
  12. Jamil, M., Lee, D. B., Jung, K. Y., Ashraf, M., Lee, S. C. & Rha, E. S. (2006). Effect of salt (NaCl) stress on germination and early seedling growth of four vegetable species. Journal of Central European Agriculture, 7, 273-282.
  13. Javaid, A. & Bajwa, R. (2011). Field evaluation of effective microorganisms (EM) application for growth, nodulation, and nutrition of mung bean. Turk Journal of Agriculture, 35,443–452.
  14.  Kafi, M. & Damghani, M. (2002). Mechanisms of Environmental Stress Resistance in Plants. Author.Basra, A.S and Basra, A.R.K. Ferdowsi University of Mashhad Press. (In Farsi).
  15. Kaplan, M., Hayek, T., Raz, A., Coleman, R., Dornfeld, L., Vaya, J. & Aviram, M. (2001). Pomegranate juice supplementation to atherosclerotic mice reduces macrophage lipid peroxidation, cellular cholesterol accumulation and development of atherosclerosis. Journal of Nutrition, 131 (8), 2082–9.
  16. Kaya, C., Higgs, D., Ince, F., Amador, B. M., Caki, A. & Sakar, E. (2003). Ameliorativeeffects of potassium phosphate onsalt-stressed pepper and cucumber. Journal of Plant Nutrition, 26, 807-820.
  17. Kusvuran, S. (2010). Relationships between physiological mechanisms of tolerances to drought and salinity in melons. Department of horticulture, institute of natural and applied sciences university of cukurova, Ph.D thesis, Adana, p. 356.
  18. Larcher, W. (1995). Physiological plant ecology (3rd ed). pp: 390. Springer Publishing.
  19. Mansour, M. M. F. (2013). Plasma membrane permeability as an indicator of salt tolerance in plants. Biologia Plantarum, 57, 1-10.
  20. Marcum, K. B. (1998). Cell membrane theromotability and whole-plant heat tolerance of Kentucky bluegrass. Crop Science, 38, 1214-1218.
  21. Mayak, S., Tirosh, T. & Glick, B.R. (2004b). Plant growth-promoting bacteria that confer resistance in tomato plant to salt stress. Plant Physiology and Biochemistry, 42,565-572.
  22. Muhammad, S., Akbar, M. & Neve, H.U. (1987). Effects of Na/Ca and Na/K ratios in salin culture solution on the growth and mineral nutrient of rice (Oriza sativa L.). Plant and Soil, 104, 57-62.
  23. Naeini, M. R., Khoshgoftarmanesh, A. H. & Fallahi, E. (2006). Partitioning of chlorine, sodium, and potassium and shoot growth of three pomegranate cultivars under different levels of salinity. Journal of Plant Nutrition, 29, 1835–1843.
  24. Nazarbeygi, E., Yazdi, H.L., Naseri, R. & Soleimani, R. (2011). The effects of different levels of salinity on proline and A-, B- chlorophylls in canola. Amer-Eurasian. Journal of Agricultural & Environmental Sciences, 10(1), 70 74.
  25. Pal, M., Singh, D. K., Rao, L. S. & Singh, K. P. (2004). Photosynthetic characteristics and activity of antioxidant enzymes in salinity tolerant and sensitive rice cultivars. Indian Journal of Plant Physiology, 9, 407-4 12.
  26. Parida, A.K. & Das, A. B. (2005). Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety, 60, 324-349.
  27. Patil, V. K. & Waghmare, P. R. (1982). Salinity tolerance of pomegranate. Journal of Maharashtra Agricultural University, 7, 268–269.
  28. Saravanakumar, D., Kavino, M., Raguchander, T., Subbian, P. & Samiyappan, R. (2011). Plant growth promoting bacteria enhance water stress resistance in green gram plants. Acta Physiologiae Plantarum, 33, 203-209.
  29. Shokouhian , A. A., Davarynejad, GH., Tehranifar, A., Imani, A. & Rasoulzadeh, A. 2013. Investigation of effective Microorganisms (EM) Impact in Water Stress Condition on Growth of Almond (prunus dulcis Mill) Seedling. Journal of Basic and Applied Scientific Research, 3(2s), 86-92.
  30. Tabatabaei, S.J. (2009). Principele of mineral nutrition of plants. Publication of Kharazmi. (In Farsi).
  31. Volkmar, K. M., Hu, Y. & Steppuhn, H. (1998). Physiological responses of plants to salinity. Areview of Canadian Journal of Plant Science, 78, 19- 27.