اثر پیش تیمار خشکی روی فعالیت آنزیم های آنتی اکسیدانی و کاهش خسارت تنش خشکی در دو گونه چمن بنت گراس خزنده Agrostis stolonifera cv. Palustris) و فستوکای پا بلند (Festuca arundinacea cv. Greystone)

نوع مقاله: مقاله کامل

نویسندگان

1 دانشجوی سابق کارشناسی ارشد دانشکده علوم کشاورزی، دانشگاه گیلان

2 دانشیار دانشکده علوم کشاورزی، دانشگاه گیلان

3 استادیار دانشکده علوم کشاورزی دانشگاه گیلان

4 استادیار دانشکده علوم کشاورزی، دانشگاه گیلان

چکیده

پیش‌تیمار خشکی، یعنی آبیاری با تکرار کم و با فواصل طولانی، که به‌منظور گسترش سیستم ریشه‏ای و کاهش خسارت خشکی در گیاهان استفاده‏ می‏شود. در پژوهش حاضر، اثر پیش‌تیمار خشکی به مدت 30 روز در افزایش تحمل به خشکی دو گونه چمن بنت‌گراس خزنده و فستوکای پابلند ارزیابی شد. گیاهان پیش‌تیمارشده و شاهد سپس به مدت 50 روز در معرض تنش خشکی با سطوح مختلف نیروی مکش خاک (20‌ـ 23، 40‌ـ 43، 50‌ـ 53 و 70‌ـ 73 سانتی‌بار) قرار گرفتند و در پایان، به‌منظور رشد مجدد، رطوبت خاک به مدت 15 روز در حد ظرفیت زراعی حفظ شد. نتایج نشان داد که پیش‌تیمار خشکی سبب افزایش نسبت طول ریشه به شاخساره در دو گونه‏ چمن شد. در پایان 50 روز تنش خشکی، میزان فعالیت آنزیم‌‌های پراکسیداز، سوپراکسید دیسموتاز، آسکوربات پراکسیداز و کاتالاز در گیاهان پیش‌تیمارشده در مقایسه با گیاهان شاهد بالاتر بود. در مرحلۀ رشد مجدد، گیاهان پیش‌تیمارشده در بنت‌گراس خزنده بر‌خلاف فستوکای پابلند، فعالیت آنزیم‏های آنتی‌اکسیدانی بالاتری از شاهد نشان دادند. در‌مجموع پیش‌تیمار خشکی با افزایش نسبت ریشه به شاخساره و تقویت سیستم‏ آنتی‌اکسیدانی، تحمل به خشکی را در هر دو گونه چمن افزایش داد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Drought Pre- conditioning on Antioxidant enzymes activity and reducing drought stress damage in two turfgrass species, creeping bentgrass and tall fescue

نویسندگان [English]

  • Kamran Amiri Nasab 1
  • Mahmood Ghasemnezhad 2
  • Hedayat Zakizadeh 3
  • Mohammad Hassan Biglouei 4
1 Faculty of Agricultural Sciences, University of Guilan
2 Faculty of Agricultural Sciences, University of Guilan
3 University of Guilan
4 Faculty of Agricultural Sciences, University of Guilan
چکیده [English]

Drought preconditioning, which is irrigation with low frequency and longer duration, could be used for extending root system and suppressing drought damage in plants. In the present study, the effect of drought preconditioning for 30 days on increasing tolerance to drought stress in two turfgrass species, creeping bentgrass (Agrostis stolonifera cv.Palustris) and tall fescue (Festuca arundinacea cv. Greystone) was investigated. Drought preconditioning and control plants were irrigated for 50 days with different levels of soil suction (20-23,40-43, 50-53 and 70-73 centibar), and finally, plants were watered up to field capacity point for 15 days, in order to recovery. The results showed that drought preconditioning increased root to shoot length ratio in both grass species. Preconditioning in both species also showed the higher leaf peroxidase, superoxide dismutase, ascorbat peroxidase and catalase activity than control plants at the end of 50 days of drought stress treatments. At recovery stage, activity of antioxidant enzymes was higher only in preconditioned creeping bent grass compared to control plants. Overall, drought preconditioning could increase drought stress tolerance in both turfgrass species by increasing root to shoot ratio and improving enzymatic antioxidant systems.

کلیدواژه‌ها [English]

  • drought tolerance
  • hardening
  • recovery
  • Root to shoot ratio
  • water stress
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