اثر نوع ریز نمونه و ترکیبات مختلف هورمونی بر باززایی مستقیم گیاه بادرنجبویه (Melissa officinalis L.)

جنگجو, خدیجه; حسنی, عباس; حسینی, بهمن; جعفری, مراد; علیزاده, مرتضی

doi:10.22059/ijhs.2015.53489

اثر نوع ریز نمونه و ترکیبات مختلف هورمونی بر باززایی مستقیم گیاه بادرنجبویه (Melissa officinalis L.)

نوع مقاله : مقاله پژوهشی

نویسندگان

¹ دانشجوی سابق کارشناسی ارشددانشکده کشاورزی دانشگاه ارومیه

² دانشیار،دانشکده کشاورزی، دانشگاه ارومیه،

³ دانشیار دانشکده کشاورزی دانشگاه ارومیه

⁴ استادیار دانشکده کشاورزی دانشگاه ارومیه

⁵ دانشجوی سابق کارشناسی ارشد دانشکده کشاورزی دانشگاه ارومیه

10.22059/ijhs.2015.53489

چکیده

بادرنجبویه (Melissa officinalis L.) گیاهی علفی، چندساله و متعلق به تیرۀ نعناعیان (Lamiaceae) است که کاربرد‏ دارویی و خوراکی وسیعی دارد. پژوهش حاضر به‏منظور بررسی پتانسیل 5 نوع ریزنمونۀ مختلف (قطعات گره، مریستم انتهایی، هیپوکوتیل، کوتیلدون و قطعات برگ) درراستای باززایی مستقیم گیاه بادرنجبویه با استفاده از غلظت‏های مختلف هورمون 6- بنزیل‏آمینوپورین (BAP) (2/2، 4/4 و 8/8 میکرومولار) با یا بدون 1 میکرومولار هورمون ایندول‏استیک اسید (IAA) به‏صورت فاکتوریل در قالب طرح کاملاً تصادفی و در سه تکرار انجام گرفت. بیشترین تعداد القای جوانه (به‏طور متوسط 59/23 و 90/16 جوانه به‏ترتیب برای ریزنمونه‏های مریستم انتهایی و قطعات گره) در غلظت 8/8 میکرومولار BAP مشاهده شد. بالاترین درصد باززایی (99درصد) در ریزنمونه‏های مریستم انتهایی و قطعات گره به‏ترتیب در محیط‏های MS تکمیل‌شده با 8/8 و 2/2 میکرومولار BAP حاصل شد. در سایر ریزنمونه‏ها هیچ‏گونه باززایی مشاهده نشد. ریشه‏زایی گیاهچه‏های باززایی‌شده در محیط‏های MS، ½ MS و MS تکمیل‌شده با 1، 5/2، 92/4 و 84/9 میکرومولار ایندول بوتیریک اسید (IBA) ارزیابی شد. بیشترین تعداد ریشة تشکیل‌شده (06/9 ریشه‏چه در گیاهچه) در محیط MS تکمیل‌شده با 92/4 میکرومولار IBA به دست آمد.‌

کلیدواژه‌ها

20.1001.1.2008482.1393.45.4.8.6

عنوان مقاله [English]

Effect of Explant Type and Different Hormonal Combinations on Direct Regeneration of Lemon balm (Melissa officinalis L.)

نویسندگان [English]

hadijeh Jangjou ¹
Abbas Hassani ²
Bahman Hosseini ³
Morad Jafari ⁴
Morteza Alizadeh ⁵

¹ Department of Horticulture-Faculty of Agriculture-Urmia University

² Department of Horticulture-Faculty of Agriculture-Urmia University

³ Department of Horticulture-Faculty of Agriculture-Urmia University

⁴ Department of Plant Breeding and Biothecnology-Faculty of Agriculture-Urmia University

⁵ Department of Horticulture-Faculty of Agriculture-Urmia University

چکیده [English]

Lemon balm (Melissa officinalis L.) is an herbaceous perennial plant of the Lamiaceae family that has a wide edible and medicinal uses. In this research the potential of five different explants (nodal segments, shoot tip, cotyledon, hypocotyl and leaf segments) on MS mediums supplemented with different concentrations of 6-Benzylaminopurine (BAP) (2.2, 4.4 and 8.8 µm) alone or in combination with 1µm Indoleacetic acid (IAA) for direct regeneration of Lemon balm was investigated. The experiment was conducted using a factorial based on completely randomized design with three replications. The maximum mean number of shoots (23.59 and 16.90 shoots in shoot tip and nodal segment explants, respectively) was observed on the MS medium with 8.8 µm BAP. The highest regeneration rate (99%) in shoot tip and nodal segment explants was achieved on the MS medium supplemented with 8.8 and 2.2 µm BAP, respectively. No shoot regeneration occurred in other explants. The rooting of regenerated shoots was assessed on MS, ½MS and MS medium supplemented with 1, 2.5, 4.92 and 9.84 µm Indole butyric acid (IBA). The highest mean number of roots (9.06 roots per explants) was observed on the MS medium supplemented with 4.92 µm IBA.

کلیدواژه‌ها [English]

Lemon balm (Melissa officinalis L.)
direct regeneration
Nodal segment
BAP
MS medium

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