نوع مقاله : مقاله پژوهشی
نویسندگان
گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Since successful fruit sets in controlled hybridization programs outside the flowering season or using pollen for production purposes at appropriate times, the presence of viable pollen grains at the time of stigma receptivity is of great importance. Considering the unique and specificity of pollen in many plant groups and the morphological and structural characteristics of pollen, in this research, the effect of the storage time (12 months) of the pollen grain of `Sebri’ cultivar and promising A95 genotype of European pear at different temperatures (laboratory ambient temperature, 4, -20 and -80°C) was investigated through culturing in a suitable medium containing sucrose (%15), boric acid (20 ppm), calcium nitrate (1.219 mM) with %0.8 agar. Their morphology was evaluated using a scanning electron microscope (SEM). The results of pollen grain culture demonstrated that the highest viability of pollen grains was observed in the A95 promising genotype at 20°C after three months of storage. Results showed that the pollen germination at different storage temperatures increased with a decrease in temperature and decreased over the storage period. The results of the morphological examination of pollen grains by SEM revealed that the studied pollen grains were monads, spheroidal in shape, radially symmetrical, and had three longitudinal furrows on their surface. The shape of the pollen grains was prolate, and their sizes varied from 35.31-39.51 micrometers.
کلیدواژهها [English]
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