نوع مقاله : مقاله پژوهشی
نویسندگان
گروه پژوهشی بیوتکنولوژی گیاهان باغبانی، پژوهشکده بیوتکنولوژی صنعتی، سازمان جهاد دانشگاهی خراسان رضوی، مشهد، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
The liquid medium offers a more consistent environment and eliminates the hassle of transferring explants during the subcultures, creating optimal conditions for plant micropropagation. To study the micropropagation of GN15 rootstock, in the first we analyzed the impact of various plant growth regulator combinations on node explant establishment in semi-solid MS culture. Then to compare the temporary immersion bioreactor and semi-solid culture conditions on propagation rate and plant health of GN15 rootstock, factors of immersion times (10 minutes every 6, 12, and 24 h), plant growth regulator concentrations (0, 0.5, and 1 mg/l BA) and sucrose concentrations (3, 4, and 5 %) in MS medium were evaluated in three separate experiments. Rooting of microcuttings was assessed in both systems with MS medium containing 0.6 mg/l IBA. The successful establishment of explants was depended heavily on plant growth regulators, MS medium containg 0.5 mg/l BA was the best. The results of propagation under two different systems demonstrated that the temporary immersion system with 10-minute immersions every 6 hours, 0.5 mg/l BA, and 3% sucrose yielded optimal growth conditions for GN15 plantlets. However, the rooting and acclimatization tests revealed that the temporary immersion system was not suitable for rooting the GN15 rootstock under the given conditions.
کلیدواژهها [English]
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