نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد، گروه کشاورزی، واحد شیروان، دانشگاه آزاد اسلامی خراسان شمالی، بجنورد، ایران

2 دانشیار، بخش تحقیقات علوم زراعی و باغی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی خراسان رضوی، سازمان ‏تحقیقات، آموزش و ترویج کشاورزی، مشهد، ایران

3 دانش‌آموخته دکتری باغبانی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی خراسان رضوی، سازمان تحقیقات، آموزش و ‏ترویج کشاورزی، مشهد، ایران

4 استادیار، گروه کشاورزی، واحد شیروان، دانشگاه آزاد اسلامی خراسان شمالی، بجنورد، ایران

10.22059/ijhs.2019.277562.1614

چکیده

این مطالعه به منظور بررسی اثر غلظتهای مختلف آگار و کربوهیدرات بر میزان پرآوری پایه پاکوتاه گیلاس (PHL-C) در شرایط درون شیشه ای و در سال 1396 انجام شد. از محیط کشت MS و آزمایش فاکتوریل با دو عامل و طرح کاملاً تصادفی در 6 تکرار و هر تکرار شامل 3 ریزنمونه استفاده شد. از تک جوانه جهت تکثیر استفاده شد. فاکتور اول، غلظت آگار در سه سطح ( 5/0، 6/0، 7/0 درصد) و فاکتور دوم غلظت کربوهیدارت شامل 6 سطح (ساکارز و شکر هر کدام در سه سطح 20، 30، 40 گرم در لیتر) بود که طی مراحل شاخه زایی، ریشه زایی و سپس سازگاری مورد ارزیابی قرار گرفت. نتایج نشان داد بین غلظتهای مختلف آگار از نظر ضریب تکثیر، درصد ریشه زایی، کیفیت گیاهچه، وزن تر گیاهچه و درصد بقا تفاوت معنی داری وجود ندارد، در سایر صفات اندازه‌گیری شده آگار با غلظت 7/0 بهترین نتیجه را داشت. به جز در صفات طول گیاهچه، تعداد برگ و وزن تر گیاهچه که ساکارز نقش پر رنگ‌تری داشته، در سایر صفات تفاوت معنی داری بین شکر و ساکارز دیده نشد. با توجه به اینکه در صفات مهم و اساسی تکثیر درون شیشه ای پایه PHL-C شکر دارای تاثیر بهتری بوده، پیشنهاد می شود جهت کاهش هزینه تولید محیط کشت، خصوصاً در تولید تجاری، از شکر معمولی و یا ترکیب شکر و ساکارز استفاده شود.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation the effect of different concentrations of agar and carbohydrate on the ‎micro-propagation of PHL-C, a dwarf cherry rootstock

نویسندگان [English]

  • Toktam Sheidayie 1
  • Ebrahim Ganji Moghadam 2
  • Shadi Attar 3
  • Morteza Azimzadeh 4

1 Former M.Sc. Student, Shirvan Branch, Islamic Azad University North Khorasan, Bojnourd, Iran

2 Associate Professor, Department of Crop and Horticulture Science Research, Khorasan Razavi, Agricultural and Natural Resources ‎Research and Education Center AREEO, Mashhad, Iran

3 Former Ph.D. Student, Khorasan Razavi, Agricultural and Natural Resources Research and Education Center AREEO, Mashhad, ‎Iran

4 Assistant Professor, Shirvan Branch, Islamic Azad University North Khorasan, Bojnourd, Iran

چکیده [English]

This study was conducted to investigate the effect of different concentrations of agar and carbohydrate on micro-propagation of dwarf cherry rootstock (PHL-C) under in-vitro conditions in 2017 year. This experiment was conducted as factorial in a completely randomized design with six replications and three plantlets in each replication. The first factor was agar in three levels (0.5, 0.6, 0.7%) and the second factor was carbohydrate in six levels (sucrose and sugar each in 3 levels of 20, 30, 40 g / l) and evaluated in proliferation, rooting and adaptation steps. The single node was used for propagation The results showed that there was no significant difference between different agar concentrations in terms of proliferation index, rooting percentage, plantlet quality, plantlet fresh weight and survival percentage, but in other measured traits, agar with concentration of 0.7 % had the best results. Except in the traits plantlet height, leaf number and plantlet fresh weight which the sucrose had a more positive effect, in other traits there was no significant difference between sugar and sucrose. Due to the fact that in the essential traits of in vitro propagation of PHL-C, sugar has a better effect, it is suggested to use table sugar or a combination of sugar and sucrose to reduce the cost of producing the culture medium, especially in commercial production.

کلیدواژه‌ها [English]

  • Proliferation index
  • rooting
  • Sucrose
  • Survival
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