تولید و ارزیابی‎ ‎قدرت‎ ‎ترکیب‌پذیری‎ ‎عمومی‎ ‎لاین‌های‎ ‎دابل‌ هاپلویید فلفل دلمه‌ای ‏‏(‏Capsicum annuum L.‎‏)‏

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه زراعت و اصلاح نباتات، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران

2 دانشیار، گروه زراعت و اصلاح نباتات، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران

3 دانشیار، بخش تحقیقات کشت بافت و سلول، پژوهشگاه بیوتکنولوژی کشاورزی ایران

چکیده

ایجاد فناوری‌های کارآمد نظیر دابل هاپلوییدی یک راه حل سریع برای تهیه لاین‌های خالص می باشد. در این تحقیق با به کارگیری روش جنین‌زایی میکروسپور لاین‌های دابل ‌هاپلوئید (اینبرد) فلفل تولید گردید. آزمون تاپ‌کراس با استفاده از 32 لاین دابل هاپلوئید تولیدی و والد تاپ‌کراس California Wonder جهت بررسی ترکیب‌پذیری عمومی لاین‌ها صورت گرفت. هیبریدهای حاصل از تلاقی لاین× ‌تستر در سال زراعی 1397-1398 کشت شدند. به منظور ارزیابی قدرت ترکیب‌پذیری عمومی لاین‌ها صفات روز تا جوانه‌زنی، روز تا گلدهی، روز تا میوه‌دهی، وزن تر میوه، وزن خشک میوه، طول میوه، عرض میوه، قطر گوشت میوه، تعداد بذر در میوه، میانگین عملکرد تک بوته، تعداد میوه در بوته و ارتفاع بوته اندازه‌گیری و تجزیه و تحلیل شدند. مقایسه میانگین‌ها توسط آزمون دانکن نشان داد نتاج تاپ‌کراس لاین‌های دابل هاپلوئید DH53, DH57, DH64, DH55  DH217, DH202,و DH90 به ترتیب با میانگین 58/8، 22/7، 18/7، 99/5، 93/5، 80/5 و 69/5 کیلوگرم در بوته بالاترین میزان عملکرد میوه را داشتند که در این میان بالاترین مقدار مربوط به نتاج تاپ‌کراس حاصل از تلاقی DH55 × California Wonder با میانگین 58/8 کیلوگرم در بوته بود. وراثت‌پذیری صفات مورد ارزیابی بالای 70 درصد بود که در این میان صفات تعداد روز تا جوانه‌زنی و میانگین عملکرد میوه در بوته بیشترین میزان را به خود اختصاص دادند. همچنین لاین‌های اشاره شده بالاترین میزان ترکیب‌پذیری عمومی مثبت و معنی دار برای صفت متوسط عملکرد در بوته را نیز به خود اختصاص دادند. بنابراین می‌توان از این 7 لاین به عنوان لاین‌های والدینی برتر در برنامه‌های اصلاحی آینده استفاده نمود.

کلیدواژه‌ها

عنوان مقاله [English]

Production and evaluation of general combining ability of doubled haploid lines in ‎sweet pepper (Capsicum annuum L.)‎‏ ‏

نویسندگان [English]

  • Azam Zarebayati 1
  • Khodadad Mostafavi 2
  • Mehran E. Shariatpanahi 3
  • Seied Mehdi Miri 2
1 Ph. D. Candidate, Department of Agronomy and Plant Breeding, Karaj Branch, Islamic Azad University, ‎Karaj, Iran
2 Associate Professor, Department of Agronomy and Plant Breeding, Karaj Branch, Islamic Azad University, Karaj, Iran
3 Associate Professor, Department of Tissue and Cell Culture, Agricultural Biotechnology Research Institute of Iran (ABRII)‎
چکیده [English]

Creating new efficient technologies such as doubled haploids is a fast solution for producing inbred lines. In this study, using the microspore embryogenesis method, doubled haploid (inbred) pepper lines were produced. The Topcross test was performed using 32 doubled-haploid lines produced and the topcross parent of California Wonder to examine the general combining ability of the lines. Hybrids derived from crosses line × tester in 2018- 2019 were planted. In order to evaluate the general combining ability of the lines, days to germination, days to flowering, days to fruit set, fresh fruit weight, dry fruit weight, fruit length, fruit width, thickness of fruit diameter, number of seeds, average yield per plant, number of fruits per plant and plant height were measured and analyzed. Comparison of means by Duncan test showed that top-cross progeny of DH55, DH64, DH57, DH53, DH217, DH202, and DH90 doubled haploid lines respectively with average of 8.58, 7.22, 7.18, 5.99, 5.93, 5.80 and 5.69 kg per plant had the highest fruit yield, among them the highest amount was related to the Topcross offset from DH55× California Wonder with an average of 8.58 kg per plant. The heritability of the evaluated traits was over 70%, among them the number of days to germination and the average fruit yield per plant were the highest. The above-mentioned lines also had the highest positive and significant general combining ability for the average yield per plant. Therefore, these 7 lines can be used as superior parent lines in future breeding programs.

کلیدواژه‌ها [English]

  • Heritability
  • sweet pepper
  • tester
  • yield‎

مراجع

  1. Adday, H. A. (2017). Estimation of heterosis, combining ability and some genetic parameters in sweet pepper. Journal of Plant Production, 8(5), 629-633.
  2. Aditika, Kanwar, H.S., Priyanka, Singh, Sh. & Singh, Sa. (2020). Heterotic potential, potence ratio, combining ability and genetic control of quality and yield traits in bell pepper under net-house conditions of NW Himalayas. Agricultural Research, 9, 526- 535.
  3. Airina, C.K., Pradeepkumar, T. & Krishnan, S. (2017). Combining ability studies exploiting gynoecy in cucumber (Cucumis sativus). Electronic Journal of Plant Breeding, 8(2), 724-731.
  4. Aiswarya, C. S., Vijeth, S., Sreelathakumary, I. & Prashant, K. (2020). Diallel analysis of chilli pepper (Capsicum annuum) genotypes for morphological and fruit biochemical traits. Plants, 9(1), 1.
  5. Chaudhary, A., Kumar, R. & Solankey, SS. (2013). Estimation of heterosis for yield and quality components in chilli (Capsicum annuum ). African Journal of Biotechnology. 12(47): 6605-6610.
  6. Çiner, D.Ö. & Tipirdamaz, R. (2002). The effects of cold treatment and charcoal on the in vitro androgenesis of pepper (Capsicum annuum). Turkish Journal of Botany, 26, 131-139.
  7. Dagnoko, S., Yaro-Diarisso, N., Sanogo, P. N., Adetula, O., Dolo-Nantoumé, A., Gamby-Touré, K., Traoré-Théra, A., Katilé, S. & Diallo-Ba, D. (2013). Overview of pepper (Capsicum) breeding in West Africa. African Journal of Agricultural Research, 8(13), 1108-1114.
  8. Devi, M.B., Pathania, N., & Thakur, N. (2018). Estimation of genetic variability, GCA and SCA effects for development of early and high yielding bell pepper hybrids suitable for protected cultivation. Journal of Applied and Natural Science, 10(1), 410-416.
  9. Enayati Shariatpanahi, M. & Emami Meybodi, D. )2009(. Microspore: Haploid cell with variety of applications in genetic and plant breeding. Modern Genetics, 3, 5-16. (In Farsi).
  10. Enayati Shariatpanahi, M. & Zare Bayti, A. (2020). Capsicum production, varieties and nutrition. Nova Science Publishers Inc. Chapter 4: 131-156.
  11. Enayati Shariatpanahi, M., Bal, U., Heberle-Bors, E. & Touraev, A. (2006). Stresses applied for the re-programming of plant microspores towards in vitro Physiologia Plantarum, 127, 519-534.
  12. Ene, C.O., Ogbonna, P.E., Agbo, C.U. & Chukwudi, U.P. (2019). Heterosis and combining ability in cucumber (Cucumis sativus). Information Processing in Agriculture, 6 (1), 150-157.
  13. Falconer, D.S. & Mackay, T.F.C. (1996). Introduction to quantitative genetics. Pearson Education, Harlow
  14. Gamborg, O.L., Miller, R.A. & Ojima, K. (1968). Nutrient requirements of suspension cultures of soyabean root cells. Experimental Cell Research, 50, 151-158.
  15. Greenleaf, W.H. (1986). Breeding vegetable crops, Pepper breeding. Basset MJ (ed.). The AVI Publishing Company Inc. Westport, Connecticut. Chapter 3: 67-134.
  16. Grubben, G.J.H. & El Tahir, I.M. (2004). “Capsicum annuum ” In: Grubben, G. J. H. and O. A. Denton (eds.). PROTA 2: Vegetables/Légumes. [CDRom]. PROTA, Wageningen, 154-163.
  17. Keshavarz, S., Bagheri, M., Ghanbari, A.A. & Mousavi, S.H. (2015). Comparison of pure lines selected from local landraces of pepper (Capsicum annuum ). Seed and Plant Improvement Journal, 31-1 (3), 403- 419. (In Farsi).
  18. Kumar, S., Kumar, R., Kumar, D., Gautam, N., Singh, N., Parkash, C., Dhiman, M.R. & Shukla, Y.R. (2017). Heterotic potential, potence ratio, combining ability and genetic control of yield and its contributing traits in cucumber (Cucumis sativus). New Zealand Journal of Crop and Horticultural Science, 45(3), 175- 190.
  19. Lemma, D. (1998). Seed production guideline for tomatoes, onion and hot pepper. IAR, Addis Ababa. 1-22.
  20. Marame, F., Dessalegne, L., Fininsa, C. & Sigvald, R. (2009). Heterosis and heritability in crosses among Asian and Ethiopian parents of hot pepper genotypes. Euphytica, 168, 235-247.
  21. Mirarab, M., Ahmadikhah, A. & Pahlavani, M.H. (2011). Study on combining ability, heterosis and genetic parameters of yield traits in rice. African Journals of Biotechnology, 10(59), 12512-12519.
  22. Miri, S.M. (2020). Artificial polyploidy in the improvement of horticultural crops. Journal of Plant Physiology and Breeding, 10(1), 1-28.
  23. Mohammadi, M., Saidi, M. & Khademi, O. (2018). Evaluation of effect of gibberellins and calcium spray in different growth stages on some qualitative and quantitative traits of sweet pepper. Iranian Journal of Horticultural Science, 48(4), 823-832. (In Farsi).
  24. Moradi Dezfouli, P., Sedghi, M., Enayati Shariatpanahi, M. & Alizade, B. (2017). Production of doubled haploid lines in rapeseed (Brassica napus) via microspore embryogenesis and evaluation of general combining ability of the lines for morphological and yield traits. Crop Biotechnology, 18, 1-14. (In Farsi).
  25. Moslemi, F.S., Olfati, J. & Hamidoghli, Y. (2019). The evaluation of cross progeny between elite lines and commercial hybrid ‘Negeen’. Iranian Journal of Horticultural Science, 50(3), 539-548. (In Farsi).
  26. Mule, P.N., Khandelwal, V., Lodam V.A., Shinde D.A., Patil P.P. & Patil A.B. (2012). Heterosis and combining ability in cucumber (Cucumis sativus). Madras Agriculture Journal, 99 (7-9), 420- 423.
  27. Nduwumuremyi, A., Tongoona, P. & Habimana, S. (2013). Mating designs: helpful tool for quantitative plant breeding analysis. Journal of Plant Breeding and Genetics, 1(3), 117-129.
  28. Niazian, M. & Shariatpanahi, M.E. (2020). In vitro-based doubled haploid production: recent improvements. Euphytica, 216 (69), 1- 21.
  29. Retes-Manjarrez, J.E., Hernández-Verdugo, S., Evrard, A. & Garzón-Tiznado, J.A. (2017). Heritability of the resistance to pepper huasteco yellow vein virus in wild genotypes of Capsicum annuum. Euphytica, 213, 275.
  30. Rodrigues, R. Gonçalves, LSA., Bento, CS., Sudré, CP., Robaina, RR. & Amaral Júnior, AT. (2012). Combining ability and heterosis for agronomic traits in chili pepper. Horticultura Brasileira 30: 226-233.
  31. Rohini, N., Lakshmanan, V., Saraladevi, D., Amalraj, J.J & Govindaraju, P. (2017). Assessment of combining ability for yield and quality components in hot pepper (Capsicum annuum). Spanish Journal of Agriculture Research, 15(2), 1-12.
  32. Shen, J., Dirks, R. & Havey, M.J. (2015). Diallel crossing among doubled haploids of cucumber reveals significant reciprocal-cross differences. Journal of the American Society for Horticultural Science, 140(2), 178-182.
  33. Sprague, G.F. & Tatum, L.A. (1942). General versus specific combining ability in single crosses of corn. Agron Journal, 34, 923- 932.
  34. Testoni, A. Eccher Zerbini, P. & Sozzi, A. (1983). Objective quality evaluation of fruit of some sweet pepper varieties for fresh consumption. In: Belletti, P., Nassi, M. O. & Quagliotti, L. (eds.) Capsicum Newsletter 2: 73-74. Institute of Plant Breeding and Seed Production. Turin, Italy
  35. Usman, Magaji G., Rafii, M.Y., Ismail, M.R., Malek, M.A. & Abdul Latif, M. (2014). Heritability and Genetic Advance among Chili Pepper Genotypes for Heat Tolerance and Morphophysiological Characteristics. The Scientific World Journal, 1-14.
علوم باغبانی ایران
دوره 52، شماره 4
بهمن 1400
صفحه 965-976

فایل ها

سابقه مقاله

  • تاریخ دریافت: 25 اسفند 1399
  • تاریخ بازنگری: 06 اردیبهشت 1400
  • تاریخ پذیرش: 28 اردیبهشت 1400

هم رسانی

ارجاع به این مقاله

آمار