مقایسۀ کمّی بیان ژن‏ های CBF1 و CBF4 تحت تنش سرما در ارقام انگور وینیفرا (Vitis vinifera L.) خلیلی دانه ‏دار، شاهرودی و گونۀ ریپاریا (Vitis riparia)

عبادی, علی; کریمی علویجه, مریم; موسوی, سید امیر; سلامی, سید علی رضا

doi:10.22059/ijhs.2015.55859

مقایسۀ کمّی بیان ژن‏ های CBF1 و CBF4 تحت تنش سرما در ارقام انگور وینیفرا (Vitis vinifera L.) خلیلی دانه ‏دار، شاهرودی و گونۀ ریپاریا (Vitis riparia)

نوع مقاله : مقاله پژوهشی

نویسندگان

¹ استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

² دانشجوی سابق دکتری پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

³ دانشیار پژوهشگاه مهندسی ژنتیک ایران

⁴ استادیار پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

10.22059/ijhs.2015.55859

چکیده

با توجه به اینکه انگور از جنبۀ اقتصادی اهمیت خاصی دارد، انجام مطالعاتی برای بررسی جنبه‏های متفاوت فرایند‏‏هایی که منجر به ایجاد مقاومت در برابر سرماهای غیر‏منتظرۀ پاییز یا دیررس بهاره می‏شود، ضروری به نظر می‏رسد. در این راستا بررسی بیان ژن‏هایی که موجب افزایش تحمل به سرما می‏شوند اهمیت ویژه‏ای دارد. لازمۀ بیان ژن‏های سنتز‏کنندۀ پروتئین‏هایی که در سازگاری به سرما نقش دارند گروهی از عوامل رونویسی‏اند. در این آزمایش تفاوت بیان دو عامل رونویسی CBF1 و CBF4 در ارقام انگور خلیلی دانه‏دار، شاهرودی و گونۀ ریپاریا تحت تنش سرما مقایسه شد. نتایج نشان داد بیان ژن CBF1 در دقایق اولیة بعد از تنش سرما افزایش نشان داد و انگور ریپاریا بیان بیشتری نسبت به دو رقم انگور وینیفرا داشت، همچنین کمترین میزان بیان ژن CBF1 مربوط به رقم شاهرودی بود که بعد از گذشت یک روز از شروع تنش مشاهده شد. در مورد ژن CBF4 افزایش بیان با گذشت ساعت‏های اولیة پس از تنش آغاز شد و مشابه ژن CBF1 بیشترین بیان مربوط به انگور ریپاریا بود. مقایسۀ میزان بیان این دو ژن در انگور ریپاریا نشان داد که به‏طور‏کلی، میزان بیان ژن CBF4 حدود 10 برابر میزان بیان ژن CBF1 بود.
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کلیدواژه‌ها

20.1001.1.2008482.1394.46.3.4.7

عنوان مقاله [English]

Quantitative expression analysis of CBF1 and CBF4 genes under cold stress treatments in grape cultivars “Khalili-Danedar”, “Shahroodi” in comparison with Vitis Riparia

نویسندگان [English]

Ali Ebadi ¹
Maryam Karimi Alvijeh ²
SeyedAmir Moosavi ³
Seyed Alireza Salami ⁴

¹ Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran

² Post Graduate Student, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran

³ Assistant Professor, Genetic Engineering Research Institute of Iran

⁴ Assistant Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran

چکیده [English]

With regard to its economic importance of grapevine, study on different aspects of factors that induce hardiness to the unexpected early-season and late-season cold seems to be essential. Some of the transcription factors are essential for synthesis of proteins that are important for cold adaptation. In the present study, differences in the expression patterns of two CBF1 and CBF4 transcription factors were evaluated under cold stress conditions in the “Khalili-Danedar”, “Shahroodi” and “Riparia”. Results showed that expression of CBF1 was increased at early minutes of cold stress and “Riparia” showed higher expression compared with two other genotypes. Also the least expression was recorded for “Shahroodi” after 24 h of cold treatment. Regarding CBF4, increase in the expression was started one hour after cold treatment and similar to the CBF1, the highest expression was recorded for “Riparia”. Results of expression patterns of these two genes in “Riparia” grape showed that expression of CBF4 was about 10 fold of CBF1.

کلیدواژه‌ها [English]

grape
transcription factors
Cold stress
gene expression
CBF1
CBF4

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