نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه کشاورزی، دانشگاه پیام نور، تهران، ایران
2 Department of Agriculture, Payame Noor University, Tehran, Iran
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
The Iranian poppy (Papaver bracteatum) is a medicinal plant known for its ability to produce the alkaloid tebaine and grows in specific regions of Iran. This study aimed to identify the optimal protein extraction method and investigate the genetic diversity of 18 populations of Iranian poppy collected from various parts of Iran, along with a control sample of Papaver somniferum, using seed protein electrophoresis patterns. The results indicated that the protein extraction method utilizing phenol yielded the best results. Additionally, after staining with Coomassie Brilliant Blue G250, the band patterns displayed higher clarity and provided greater precision in tracking protein bands. A total of 68 bands were observed within a molecular weight range of 120-13 kDa when comparing electrophoresis patterns. The least and most number of bands were recorded in populations P2 (Lar Dam, Alborz) and P17 (Pol Zanguleh region, Alborz), respectively. Genetic similarity among the studied populations varied significantly, ranging from 0.4 to 0.8. The greatest distance was noted between populations P15 and P7 from two different regions: Yush-Baladeh village in Alborz and Pir-Imran village in Kurdistan. Seed protein electrophoresis effectively distinguished between species P.bracteatum and P.somniferum. Cluster analysis using UPGMA method along with Dice similarity coefficient revealed five distinct groups among the studied populations. The findings suggest that geographic location has minimal impact on population similarity and their clustering; therefore, seed protein electrophoresis can be utilized for isolating and identifying different populations of this species in conservation programs.
کلیدواژهها [English]
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