نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه خوارزمی، تهران، ایران.
2 بخش بیولوژی مولکولی، پژوهشکده تحقیقات بیوتکنولوژی کشاورزی منطقه شمال کشور، رشت، ایران.
3 بخش بیولوژی مولکولی، پژوهشکده تحقیقات بیوتکنولوژی منطقه شمال کشور، رشت، ایران
4 گروه ژنتیک و بهنژادی، پژوهشکده مرکبات و میوه‌های نیمه‌گرمسیری، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی،
5 گروه فیزیولوژی و فناوری پس از برداشت، پژوهشکده مرکبات و میوه‌های نیمه گرمسیری، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
The aim of this research was to obtain triploid acid citrus genotypes. Stability of polyploidy level for two genotypes of key lime labeled as A and B and three genotypes of sweet lime labeled as C, D and E were investigated. The results showed that in tree A, four of five main branches (A1, A2, A3 and A4) were diploid (2n=2X) and only one main branch (A5) was mix-ploid (2X+4X). In tree B, all of seven main branches were equally triploid (2n=3X). All branches of C and D trees of sweet lime were tetraploid and those of E tree were diploid. Controlled crossing was made between clementine mandarin and B tree of key lime and between each of three female parents of lemon (ʻEurekaʼ, ʻCook Eurekaʼ and ʻLisbonʼ) and two sweet lime tetraploids (C and D trees). From crossing between clementine mandarin and Mexican lime (B tree) no fruit as well as no seed was obtained. However, from crosses of three female lemons (ʻEurekaʼ, ʻCook Eurekaʼ and ʻLisbonʼ) with tetraploid sweet limes fruits and seeds were obtained. Only very small seeds with high monoembryony were cultured on B5 medium. Fifteen interploid hybrids were analyzed for ploidy level by flowcytometry of which ten seedlings were diploid (nucellar) and five seedlings were triploid (2n=3X), four from ʻEurekaʼ × sweet lime and one from ʻCook Eurekaʼ × sweet lime. The triploid genotypes obtained for the first time here are supposed to be seedless, therefore should be more evaluated for phenotypic characteristics.
کلیدواژه‌ها [English]
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