Pre-harvest application of chitosan and Aloe vera gel coating on biochemical ‎parameters and post-harvest life of ‘Asgari’ grapes

Document Type : Full Paper


1 Associate Professor, Agriculture Faculty, Lorestan University, Khorramabad, ‎Khorramabad, Iran

2 Ph. D. Candidate, Agriculture Faculty, Lorestan University, Khorramabad, ‎Khorramabad, Iran

3 Graduate M. Sc. Student, Agriculture Faculty, Lorestan University, Khorramabad, Khorramabad, Iran


The use of biodegradable films and edible coatings with antimicrobial properties as a suitable alternative to synthetic preservatives is important in order to maintain food security and prevent capital wastage. In this respect, the effect of pre-harvest chitosan spraying (at three concentrations of 0, 2 and 3%) and post-harvest immerging in Aloe vera gel (25 and 33%) on the quality and shelf life of ‘Asgari’ grape fruit in five different times (0, 7, 14, 21 and 28 days after harvest) at 4 °C was evaluated. The results showed that berries treated with chitosan and Aloe vera gel contained higher content of vitamin C, soluble solids, Total flavonoids content, peroxidase activity and shelf life and lower content of malondealdehide (MDA) than the control, as well as, polyphenol oxidase enzyme activity and weight loss percentage were lower in fruits treated with chitosan and aloe vera gel.The highest amount of vitamin C, soluble solids and peroxidase activity in all five measurements belonged to the combined treatments of chitosan 2% and Aloe gel 25% and the highest flavonoid content and fruit shelf life was observed in the treatment of chitosan 3% with Aloe gel 25%. In all five measurements, it was observed that 2% chitosan treatment with 25% Aloe gel had the lowest weight loss, polyphenol oxidase activity and MDA.


Main Subjects

  1. Abassi, N., Kushad, M., & Endress, A. (1998). Active oxygen-scavenging enzymes activities in developing apple flowers and fruits. Scientia Horticulturae, 74(3), 183-194.
  2. Adiletta, G., Zampella, L., Coletta, C., & Petriccione, M. (2019). Chitosan coating to preserve the qualitative traits and improve antioxidant system in fresh figs (Ficus carica). Agriculture. 9(84), 193-213.
  3. Ali, A., Maqbool, M., Ramachandran, S., Alderson, P.G. (2010). Gum arabic as a novel edible coating for enhancing shelf-life and improving postharvest quality of tomato (Solanum lycopersicum) fruit. Postharvest Biological Technology, 58 (1), 42-47.
  4. Asadi, Z., Beig Mohamadi, Z. and Mirmajidi, A. (2020). Investigate of the effect of the effect of food coating containing spirulina platensis, chitosan and gelatin on physicochemical, sensory and nutritional properties of dried kiwi. Food Science and Technology, 102 (17), 68-53. )In Farsi(.
  5. Ashraf, M., Shaheen, Sh., Naseer, S., & Akram, N. A. (2013). Salt stress affects water relations, photosynthesis, and oxidative defense mechanisms in (Solanum melongena). Journal of Plant Interactions, 8(1), 85-96.
  6. Bako, S. P., & Adams, F. (2005). Respiratory weight loss in yam (Dioscorea rotundata Poir) tubers, fruits of Valencia oranges (Citrus sinensis) and tomato (Lycopersicon esculentum Mill.) stored using plant derived materials as protective coating in Zaria, Nigeria. International Journal of Botany, 1(2), 143-146.
  7. Buege, J.A., & Aust, S.D. (1978). Microsomal lipid peroxidation. Methods Enzyme, 52, 302-310.
  8. Corbo, M.R., Speranza, B., Campaniello, D., D’Amato, D., Sinigaglia, M. (2010). Fresh-cut fruits preservation: current status and emerging technologies. Microbial Biotechnology. 15(25), 1143-1154.
  9. Ding, P. (2013). Physico-chemical changes in dabai (Canarium odontophyllum) fruit during modified atmosphere storage. International Food Research Journal, 20(6), 3033-3040.
  10. Dong, H., Cheng, L., Tan, J., Zheng K., & Jiang, Y. (2004). Effects of chitosan coating on quality and shelf life of peeled litchi fruit. Journal of Food Engineering, 64, 355-358.
  11. Duan, J., Wu, R., Stric, B. C., & Zhao, Y. (2011). Effect of edible coatings of fresh blueberries (Duke and Elliott) under commercial storage conditions. Postharvest Biology and Technology, 59(1), 71-79.
  12. Ehtesham Nia, A., Taghipour, S., & Siahmansour, S. (2021). Pre-harvest application of chitosan and postharvest Aloe vera gel coating enhances quality of table grape (Vitis vinifera cv. ‘Yaghouti’) during postharvest period. Food Chemistry, 347 (2021), 129012.
  13. Galindo, F. G., Herppich, W., Gekas, V., & Sjöholm, I. (2004). Factors affecting quality and postharvest properties of vegetables: Integration of water relations and metabolism. Critical Reviews in Food Science and Nutrition, 44(3), 139-154.
  14. Ghasi Mogadam, M., Salahvarzi, Y., & Abedi, B. (2020). Increasing the shelf life and preserving of the quality of apple fruit ̔ Golab̕ using an edible coating of Aloe vera gel and essential oil of Shirazi thyme, Iranian Journal of Horticultural Sciences, 51 (4), 911-899. (In Farsi).
  15. Han, C., Zuo, J., Wang, Q., Xu, L., Zhai, B., & Wang, Z. (2014). Effects of chitosan coating on postharvest quality and shelf life of sponge gourd (Luffa cylindrica) during storage. Scientia Horticulturae, 55(16), 166 - 178.
  16. Harish Prashanth, K. V., Dharmesh, S. M., Jagannatha Rao, K. S., & Tharanathan, R. N. (2007). Free radical-induced chitosan depolymerized products protect calf thymus DNA from oxidative damage. Carbohydrate Research, 342, 190-195.
  17. Heidar nejad, R., Ghahramani, Z., Barzegar, T., & Rabiee, V. (2020). The effect of harvest stage and storage duration on fruit quality of Physalis (Physalis angulate). Journal of Agricultural Crops Production, 5 (1), 186-173. (In Farsi).
  18. Jain, V., Chawla, Sh., Choudhary, P., & Jain, S. (2019). Post-harvest calcium chloride treatments influence fruit firmness, cell wall components and cell wall hydrolyzing enzymes of Ber (Ziziphus mauritiana) fruits during storage. Journal of Food Science and Technology, 56(10), 4535-4542.
  19. Kar, M., & Mishra, D. (1976). Catalase, Peroxidase and polyphenol oxidase activities during rice leaf senescence. National library of Medicine, 57 (2), 315-319.
  20. Karimi, M., Hoseini, M., & Zahedi, M. (2018). The Effect of Postharvest Chitosan Treatment on the Quality Maintenance of Banana (Musa acuminata Cavendish) during Cold Storage. Journal of Crop production and processing, 8(1), 14-1. (In Farsi).
  21. Kou, X.H., Guo, W.L., Guo, R.Z., Li, X.Y., & Xue, Z. H. (2013). Effects of chitosan, calcium chloride, and pullulan coating treatments on antioxidant activity in pear cv. “Huang guan” during storage. Food Bioprocess Technology, 7, 671-681.
  22. Kumar, P., Sethi, S., Sharma, V. V., Srivastav, M., & Varghese, E. (2017). Effect of chitosan coating on postharvest life and quality of plum during storage at low temperature. Scientia Horticulturae, 226, 104-109.
  23. Lin, Y. F., Lin, H. T., Zhang, S., Chen, Y. H., Chen, M. Y., & Lin, Y. X. (2014). The role of active oxygen metabolism in hydrogen peroxide-induced pericarp browning of harvested longan fruit. Postharvest Biology and Technology, 96, 42-48.
  24. Liu, Y., Cui, Y., & Mukherjee, A. (2007). Characterization of a novel RNA regulator of Erwinia carotovora spp. Carotovora that controls production of extracellular enzymes and secondary metabolites. Molecular Microbial, 29(1), 219-34.
  25. Lustriane, C., Dwivany, F., Suendo, V., & Reza, M. (2018). Effect of chitosan and chitosan-nanoparticles on postharvest quality of banana fruits. Journal of Plant Biotechnology, 45, 36-44.
  26. Maghsoodi, V. (2003). Effect of vanillin on germination time and radial growth of moulds in apple puree. Iran Agricultural Research. 22 (2), 153-164. (In Farsi).
  27. Mendy, T.K., Misran, A., Mahmud, T.M.M., & Ismail, S.I. (2019). Application of Aloe vera coating delays ripening and extend the shelf life of papaya fruit. Scientia Horticulturae, 246, 769-776.
  28. Meng, X. Li, B. Liu, J., & Tian, Sh. (2008). Physiological responses and quality attributes of table grape fruit to chitosan preharvest spray and postharvest coating during storage. Food Chemistry, 106, 501-508.
  29. Moradi Ganjeh, F., Meamar Dastjerdi, R., Heydari, M., & Movahed Nezhad, M. A. (2020). The effect of Chitosan-clay nano composite, wax coatings and olive oil on some quality properties of sweet lemon during shelf-life storage. Agricultural Engineering (Scientific Journal of Agriculture), 43 (3), 95-112.
  30. Nguyen, D. H., & Nguyen, V. H. (2020). Effect of nano-chitosan coating on the quality, polyphenol oxidase activity, MDA content of strawberry. Journal of horticulture and postharvest research, 3 (1), 11-24.
  31. Parsa, J., Smaeel Amiri, M., Hajiloo, J., Razavi, F., & Rahnemoun, H. (2019). Effect of Aloe vera gel on physiologiacal and biochemical triats of two cultivars of apricot in storage. Journal of Food Industry Research, 30 (3), 203-219. (In Farsi).
  32. Pasquariello, M. S., Rega, P., Migliozzi, T., Rita Capuano, L., Scortichini, M., & Petriccione, M. (2013). Effect of cold storage and shelf life on physiological and quality traitsof early ripening pear cultivars. Scientia Horticulturae, 162, 341-350.
  33. Pradeep, A. R., Agarwal, E., & Naik, S. B. (2012). Clinical and microbiologic effects of commercially available dentifrice containing aloe vera: a randomized controlled clinical trial. Journal of periodontology, 83(6), 797-804.
  34. Ricoa, D., Martin-Dianaa, A. B., Baratb, J. M., & Barry-Ryan, C. (2007). Extending and measuring the quality of fresh-cut fruit and vegetables: a review. Trends in Food Science and Technology, 18, 373-386.
  35. Romanazzi, G., Feliziani, E., Baños, S. B., & Sivakumar, D. (2017). Shelf life extension of fresh fruit and vegetables by chitosan treatment. Critical reviews in food science and nutrition, 57(3), 579-601.
  36. Saki, M., ValizadehKaji, B., Abbasifar, A., & Shahrjerdi, I. (2019). Effect of chitosan coating combined with thymol essential oil on physicochemical and qualitative properties of fresh fig (Ficus carica) fruit during cold storage. Journal of Food Measurement and Characterization, 13(2), 1147-1158.
  37. Shiri, M. A., Ghasemnezhad, M., Bakhshi, D., & Saadatian, M. (2011). Effect of ascorbic acid on phenolic compounds and antioxidant activity of packaged fresh cut table grape. Electronic Journal of Environmental, Agricultural and Food Chemistry, 10, 2506-2515.
  38. Shiri, M. A., Ghasemnezhad, M., Fattahi Moghaddam, J., & Ebrahimi, R. (2016). Effect of CaCl2 sprays at different fruit development stages on postharvest keeping quality of ‘Hayward’ kiwifruit. Journal of Food Processing and Preservation, 40(4), 624-635.
  39. Sogvar, O.B., Koushesh Saba, M., & Emamifar, A. (2016) Aloe vera and ascorbic acid coatings maintain postharvest quality and reduce microbial load of strawberry fruit. Postharvest Biology and Technology, 114, 29-35.
  40. Tabatabaekoloor, R. (2012). Orange responses to storage conditions and polyethylene wrapped liner. Agricultural Engineer International: CIGR Journal, 14(2), 127-130.
  41. Taduri, M., Reddy, N. N., Lakshmi, J., & Joshi, V. (2017). Effect of pre-harvest treatments on shelf life and quality of mango CV Amrapali. Journal of Pharmaceutical Innovation, 6(7), 54-59.
  42. Torrigiani, P., Bregoli, A. M., Ziosi, V., Scaramagli, S., Ciriaci, T., Rasori, A., Biondi, S., & Costa, G. (2004). Pre-harvest polyamine and aminoethoxyvinylglycine (AVG) applications modulate fruit ripening in Stark Red Gold nectarines (Prunus persica Batsch). Postharvest Biology and Technology, 33, 293-308.
  43. Tsaniklidis, G., Delis, C., Nikoloudakis, N., Katinakis, P., & Aivalakis. G. (2014). Low temperature storage affects the ascorbic acid metabolism of cherry tomato fruits. Plant Physiology and Biochemistry, 84, 149-157.
  44. Xie, W. M., Xu, P. X., & Liu, Q. (2001). Antioxidant activity of water-soluble chitosan derivatives. Bioorganic and Medicinal Chemistry Letters, 11, 1699-1701.
  45. Xing, Y., Li, X., Xu, Q., Yun, J. and Tang, Y. (2011). Effects of chitosan coating enriched with cinnamon oil on qualitative properties of sweet pepper (Capsicum annuum). Journal of Food Chemistry. 124, 1443-1450
  46. Xing, Y., Yang, S., Xu, Q., Xu, L., Zhu, D., Li, X., Shui, Y., Liu, X., & Bi, X. (2021). Effect of Chitosan/Nano-TiO2 composite coating on the postharvest quality of blueberry fruit. Coatings, 11, 512.
  47. Zheng, X., & Tian, S. (2006). Effect of oxalic acid on control of postharvest browning of litchi fruit. Food Chemistry, 96(4), 519-523. foodchem.2005.02.049.