Iranian Journal of Horticultural Science

Iranian Journal of Horticultural Science

Evaluation of some characteristics of the fourth generation of common purslane ‎‎(Portulaca oleracea L.) mutated from treatment with dimethyl sulfate ‎

Document Type : Full Paper

Authors
Maryam Nasrollahi Pari 1
Mohammad Hossein Fotokian 2
1 Graduated M. Sc. Student , Faculty of Agriculture, Shahed University, Tehran, Iran
2 Associate Professor, Faculty of Agriculture, Shahed University, Tehran, Iran
10.22059/ijhs.2022.332903.1979
Abstract
Mutation induction is an important method for generating genetic diversity. Purslane (Portulaca oleracea L.) has unique medicinal uses due to its high content of beneficial antioxidants and omega-3 fatty acids. In this study, the genetic diversity of 24 mutant lines of fourth-generation (M4) purslane obtained from dimethyl sulfate treatment together with the control was investigated in a randomized complete block design with 3 replicates. The differences among the lines were significant in traits including plant height, leaf length, leaf width, stem dry weight, and leaf chlorophyll in the 30-day-old plant. The principal coordinates and cluster analyses divided the lines into two separate clusters. The lines in cluster 1 had a significant advantage over the lines in cluster 2 for most traits such as stem fresh weight, leaf fresh weight, and number of capsules per plant. The number of leaves was significantly correlated with stem fresh weight, leaf fresh weight, plant fresh weight, number of capsules per plant and the ratio of plant dry weight to plant fresh weight. In sequential path analysis, stem fresh weight in the first order was the only trait affecting number of capsules per plant, and in the second-order, leaf fresh weight and plant height were the most important traits that had an effect on number of capsules per plant through the direct effect of stem fresh weight. L18 mutant line had a significant advantage over the control in some agronomic traits such as plant height, leaf length and number of capsules per plant, that can be used for future research, especially for cultivation, commercialization and extensive planting for uses as vegetables, oil extraction, and medicinal applications.
Keywords
Mutation induction
principal coordinate analysis
sequential path analysis
variance components
Subjects
  1. Ahmadi, M., Omidi, M., Alizadeh, B., & Shahnejat Bushehri, A.A. (2017). Evaluation of canola mutant lines (Brassica napus) comparing to their parents in order to select superior lines. Modern Genetics Journal, 12 (2), 157-166. (In Farsi).
  2. Alahbakhsh, E., Galavi, M., Mosavi, M., & Mohkami, Z. (2020). Effects of irrigation regimes and fertilizer on oil percent and fatty acid composition ‎leaf and seed purslane (Portulaca oleraceae)‎, Iranian Journal of Horticultural Science, 51 (2), 375-386. (in Farsi).
  3. Farahani, A., & Arzani, A. (2008). Investigation of genetic diversity of durum wheat genotypes by multivariate statistical analysis. Crop Production (Electronic Journal of Crop Production), 1 (4), 51-64. (In Farsi).
  4. Fotokian, M.H. (1992). Investigation of the effects of gamma ray and dimethyl sulfate (DMS) on several varieties of rice.Sc. Thesis, Faculty of Agriculture. Tabriz University, Tabriz, Iran.(in Farsi).
  5. Fotokian, M.H., & Feizi Kelasi, N. (2020). Investigation of genetic diversity on M2 mutant lines of Purslane (Portulaca oleracea) derived through DMS mutagenes. Journal of Crop Breeding, 12 (34), 105-114. (In Farsi).
  6. Hajzadeh, M.R., Rakhshandeh, H., Esmaeilizadeh, M., & Ghorbani, A. (2004). Analgesic and anti-inflammatory effects of Portolaca oleracea extracts in mice & rat. Koomesh Journal, 5 (3), 113-120.
  7. Hamedi, S., Forouzanfar, F., Rakhshandeh, H., & Arian, A. (2019). Hypnotic Effect of Portulaca oleracea on Pentobarbital-Induced Sleep in Mice. Current Drug Discovery Technologies, 16 (2), 198-203.
  8. Hashemi, B., Taghiloo, S., Allahmoradi, E., Karami, M., & Rahdar, H.A. (2018). Assessment of antibacterial effect of hydro-alcoholic extract of Portulaca oleracea on the human pathogen bacteria. Journal of Sabzevar University of Medical Sciences, 25 (3), 303-308. (In Farsi).
  9. Hesarinejad, M.A., Abdollahi Moghaddam, M.R., Jafarzadeh, M., & Rezaee Oghazi, M. (2021). The study of physicochemical and antioxidant properties of encapsulated Portulaca oleracea aqueous extract prepared by spray drying method. Innovative Food Technologies, 8 (3), 325-335. (In Farsi).
  10. Hosseinava, S., & Hajnajari, H. (2008). Preliminary study for possible producion of mutants using mutagenic chemical compunds in some olive (Olea europea ) cultivars. Seed and Plant Journal, 24 (1), 33-43. (In Farsi).
  11. Hosseinzadeh, M. H., Ghalavand, A., Boojar, M.M.A., Modarres-Sanavy, S.A.M., & Mokhtassi-Bidgoli, A. (2020). Effect of deficit irrigation, mycorrhiza and nitrogen nutrition system on some chemical properties of soil, oil content and biological yield of purslane (Portulaca oleracea). Iranian Journal of Field Crop Science, 51 (2), 29-48. (In Farsi).
  12. Iranshahy, M., Javadi, B., Iranshahi, M., Jahanbakhsh, SP., Mahyari, S., Hassani, F.V., & Karimi, G. (2017). A review of traditional uses, phytochemistry and pharmacology of Portulaca oleracea Journal of Ethnopharmacology, 205, 158-172. (In Farsi).
  13. Kakaei, M. (2018). Evaluation of allelic diversity using SDS-PAGE technique in leaf tissues of bread wheat. Iranian Journal of Plant Biology, 9 (4), 25-38. (In Farsi).
  14. Karimi, A. (2018). Effect of purslane (Portulaca oleracea) on CRP, TNF-Α, hepatic enzymes and serum nitrate in rats with experimental allergic encephalomyelitis. The Quarterly Journal of Animal Physiology and Development, 11 (4), 65-74. (In Farsi).
  15. Mohammadi, S., Tabrizi, L., Shokrpour, M., Hadian, J., & Schulz, H. (2020). Study on general combining ability of morphological and phytochemical traits in ‎different populations of Thymus daenensis in polycross test. Iranian Journal of Horticultural Science, 51 (2), 295-305. (In Farsi).
  16. Nasisir, F., & Saeidi, Gh. (2013). Evaluation of genetic diversity in breeding lines derived from native sesame cultivars. Iranian Journal of Field Crops Research, 10 (4), 659-666. (In Farsi).
  17. Nemzer, B., Al-Taher, F., & Abshiru, N. (2020). Phytochemical composition and nutritional value of different plant parts in two cultivated and wild purslane (Portulaca oleracea) genotypes. Food Chemistry, 320, 126621.
  18. Pahlavani, M.H., Nemati, M., & Faraji, A. (2011). Quantitative and biometric genetic techniques in plant breeding. Faraghi Publications in collaboration with Gorgan University of Agricultural Sciences and Natural Resources. (In Farsi).
  19. Rahimi, F. (2018). Evaluation of genetic diversity in M3 lines of purslane (Portulaca oleracea), M.Sc. Thesis, Faculty of Agriculture, Shahed University, Tehran. Iran. (in Farsi).
  20. Rajabi, A., Gharib bolouk, S., Kazemitabar, SK., & Sinaki, J.M. (2016). Study of peppermint mutant plant traits using mutagen EMS mutagenesis. New Cellular and Molecular Biotechnology Journal, 6 (24), 87-96. (In Farsi).
  21. Sabaghnia, N., Dehghani, H., Alizadeh, B., & Mohghaddam, M. (2010). Interrelationships between seed yield and 20 related traits of 49 canola (Brassica napus ) genotypes in non-stressed and water-stressed environments. Spanish Journal of Agricultural Research, (2), 356-370.
  22. Shah Mohammadi, A. (2016). The effect of different concentrations of dimethyl sulfate on purslane (Portulaca oleracea L.). M.Sc. Thesis, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran. Iran (in Farsi).
  23. Sultana, A., & Rahman, K. (2013). Portulaca oleracea A global Panacea with ethno-medicinal and pharmacological potential. Internationa Journal of Pharmacy Pharmaceutical Sciences, 5, 33-39.
  24. Tangsombatvitchit, C., Wongpiyasatid, A., Jompuk, P., & Taychasinpitak, T. (2008). Effects of acute gamma irradiation on mutation from stem cuttings of Portulaca oleracea Agricultural Science Journal, 39(1), 55-64.
  25. Wainstein, J., Landau, Z., Dayan, Y.B., Jakubowicz, D., Grothe, T., Perrinjaquet-Moccetti, T., & Boaz, M. (2016). Purslane extract and glucose homeostasis in adults with type 2 diabetes: A double-blind, placebo-controlled clinical trial of efficacy and safety. Journal of Medicinal Food, 19 (2), 133-140.
  26. Walter, J., Vekslyarska, T., & Dobes, C. (2015). Flow cytometric, chromosomal and morphometric analyses challenge current taxonomic concepts in the Portulaca oleracea complex (Portulacaeae, Caryophyllales). Botanical Journal of the Linnean Society, 179, 144-156.
  27. Xiang, L., Xing, D., Wang, W., Wang, R., Ding, Y., & Du, L. (2005). Alkaloids from Portulaca oleracea Phytochemistry, 66 (21), 2595-2601.
  28. Zhou, Y-X., Xin, H-L., Rahman, K., Wang, S-J., Peng, C., & Zhan, H. (2015). Portulaca oleracea: A Review of phytochemistry and pharmacological effects. BioMed Research International, 2015:925631.10.1155/2015/925631.
Iranian Journal of Horticultural Science
Volume 53, Issue 4
Winter 2023
Pages 881-890

  • Receive Date 05 November 2021
  • Revise Date 08 December 2021
  • Accept Date 09 June 2022