In vitro propagation and acclimatization of Adenium sp. as valuable ornamental ‎plant

Document Type : Full Paper


1 Former M. Sc. Student, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran

2 Assistant Professor, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran


Optimizing the tissue culture condition for Adenium sp as an imported ornamental plant with a low propagation rate can deliver the way for mass propagation to multiplication of this ornamental plant. So, this research was carried out in a factorial experiment based on a completely randomized design to optimize organogenesis of Adenium abessum and Adenium arabicum during shoot induction, proliferation and root induction experiments. The highest rate of shoot regeneration for Adenium obesum was achieved in MS medium containing 0.5 mgl-1 BA, and for Adenium arabicum was achieved in MS medium containing 0.5 mgl-1 BA in combination with 0.5 mgl-1 TDZ. Organogenesis was more observed as leafy and rooted in A. arabicum and shoot induction in that was longer compare with A. abessum. The result showed that 0.5 mgl-1 BA can be used to prolifered shoots induced in both specie of Adenium. The full strength of free plant growth regulator MS medium supplemented with 0.3% activated charcoal was the best treatment at root induction stage. The plantlet gradually became first adapted in sterile perlite with 1/2 MS concentration and then in the culture media contained soil, vermicompost and perlite.


  1. Beasley, R. R. & Pijut, P. M. (2013). Regeneration of plants from Fraxinus nigra Marsh. hypocotyls. Horticultural Science, 48, 887-890.
  2. Bonyanpour, A. & Khosh-Khui, M. (2013). Callus induction and plant regeneration in Punica granatum L. ʻNanaʼ from leaf explants. Journal of Central European Agriculture, 14(3), 75-83.
  3. Chen, J. & Wei, X. (2018). Thidiazuron in micropropagation of aroid plants. Thidiazuron: From Urea Derivative to Plant Growth Regulator. (pp. 95-113). Springer, Singapore.
  4. Davey, M. R., Anthony, P., Power, J. B. & Lowe, K. C. (2005). Plant protoplasts: status and biotechnological perspectives. Biotechnology Advances, 23, 131-171.
  5. Deein, W., Thepsithar, C. & Thongpukdee, A. (2013). In vitro culture meddium sterilization by chemicals and essential oils without autoclaving and growth of Chrysanthemum Nodes. International Journal of Bioengineering and Life Sciences, 7(6), 407-410.
  6. Dimmitt, M. A. & Hanson, C. (1991). The genus Adenium in cultivation. Diplorhynchus Welw Fic & Hiern (Apocynaceae), Mededelingen. Cactus and Succulent Journal, 63, 223-225.
  7. Heinz, D. J. & Mee, G. W. P. (1969). Plant differentiation from callus tissue of Saccharum species. Crop Science, 9, 346-348.
  8. Huetteman, C. & Preece, J. (1993). Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell, Tissue and Organ Culture, 33, 105-119.
  9. Kanchanapoom, K., Sunheem, S. & Kanchanapoom, K. (2010). In vitro propagation of Adenium obesum (Forssk.) Roem and Schult. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38(3), 209-213.
  10. Lee, J. H. & Pijut, P. M. (2017). Adventitious shoot regeneration from in vitro leaf explants of Fraxinus nigra. Plant Cell Tissue Organ Culture, 130, 335-343.
  11. Li, J. J., Wu, Y. M., Wang, T. & Liu, J. X. (2009). In vitro direct organogenesis and regeneration of Medicago sativa L. Biologia Plantarum, 53, 325-328.
  12. Lim, Z. X., Ling, A. P. K. & Husseni, S. (2009). Callus induction of Ocimum sancatum and estimation of its total flavonoids content. Asian Journal of Agriculture Science, 1, 55-61.
  13. Madani, G., Ghobadi, S., Seyed-Tabatabaei, B. E., Talebi, M. & Yamchi, V. (2013). Effect of plant growth regulators and explant types on regeneration and micropropagation of a commercial strawberry cultivar (Fragaria×ananassa cv. Selva). Journal of Science and Technology of Greenhouse Culture, 15, 111-122. (in Farsi)
  14. Mehrabani, B., Nazeri, S. & Piri, K. (2013). Effect of BA and NAA hormones on shoot regeneration and total Phenol and Flavonoied compounds of Chaei Koohi (Stachy slavandulifolia Vahi.) in vitro culture. Agricultural Biotechnology, 4(1), 1-9. (in Farsi)
  15. Oyen, L. P. (2008). Adenium obesum (Forssk.) Roem. & Schult. Plant Resources of Tropical Africa, 11(1), 46-49.
  16. Peres, L. E. P., Majerowicz, N., Gilberto, E. & Kerbauy, B. (2001). Dry matter partitioning differences between shoots and roots in Two contrasting genotypes of Orchids and their relationship with endogenous levels of Auxins, Cytokinins and Abscisic acid. Departamento de Botanica, 5, 422-970.
  17. Rasad, F. M., Hasbullah, N. A., Daud, N. F., Azis, N. A., Amin, M. A. M. & Lassim, M. M. (2015). Micropropagation of Adenium obesum (Desert Rose) In VitroInternational Conference on Agricultural, Ecological and Medical Sciences, 7 April., Phuket, Thailand. Pp. 10-12.
  18. Schenk, R. U. & Hilderandt, A. C. (1972). Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Canada Journal of Botany, 50, 199-204.
  19. Sorin, C., Bussell, J., Camus, I., Ljung, K., Kowalczyk, M., Geiss, G., McKhann, H., Garcion, C., Vaucheret, H., Sandberg, G. & Bellini, C. (2005). Auxin and light control of adventitious rooting in Arabidopsis require Argonaut. The Plant Cell, 17(5), 1343-1359.
  20. Srivastava, L. M. (2002). Plant growth and development: hormones and environment. Elsevier.
  21. Taghizadeh, M. & Solgi, M. (2015). Introduction of commercial protocol for in vitro proliferation of Brassica oleracea var. acephala. Iranian Journal of Horticultural Science, 45(4), 475-484. (in Farsi)
  22. Talukdar, T. (2012). Deveiopment of Nacl-tolerant line in an endanger ornamental Adenium multiflorum klotzsch in vitro selection. International Journal of Recent Scientific Research, 813-821.
  23. Thrope, T. A. (1981). Plant tissue culture: methods and applications in agriculture. New York, Academic Press.
  24. Voisey, C. R., White, D. W, Dudas, B., Appleby, R. D., Ealing, P. M. & Scott, A. G. (1994). Agrobacterium-mediated transformation of white clover using direct shoot organogenesis. Plant Cell Reports, 13, 309-314.
  25. Wei, Z., Qiang, F., Xigang, D. & Manzhu, B. (2008). The culture of isolated microspores of ornamental kale (Brassica oleracea var. acephala) and the importance of genotype to embryo regeneration. Scientia Horticulturae, 117, 69-72.
  26. Win, N. K. K., Back, C. G., Kim, Y. H. & Jung, H. Y. (2012). Desert rose witches’ broom disease associated with ‘Candidatus Phytoplasma aurantifolia’. Journal of General Plant Pathology, 78(1), 73-76.