Optimization in vitro micropropagation of peony (Paeonia mascula) ‎

Document Type : Full Paper


1 Ph.D. Candidate, Department of Horticultural Sciences, University Campus, University of Guilan, Rasht, Iran

2 Assistant Professor, Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

3 Professor, Research Institute of Forests and Rangelands, Tehran, Iran

4 Assistant Professor, Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, ‎Evin, Tehran, Iran


This research was conducted in two stages to optimize in vitro propagation of Persian wild paeony. Stage 1: effect of BAP (0, 0.5, 1 and 2 mg.l-1), NAA (0, 0.1, 0.2 and 0.3 mg.l-1) and GA3 (0 and 0.5 mg.l-1) on apical bud ofPaeonia mascula and stage 2: effect of BAP (0, 0.5, 1 and 2 mg.l-1) and NAA (0, 0.25, 0.5 and 1 mg.l-1) were studied on embryonic callus induction on young leaves of Paonia mascula. At stage 1: the highest shoots number (5.38) was obtained on media containing 1 mg.l-1 BAP + 0.1 mg.l-1 NAA+ 0.5 mg.l-1 GA3. The results showed that increasing BAP level caused decreasing of shoot length. At stage 2: maximum of compact embryonic callus (17.93%) and somatic embryo number per explant (7.11) were obtained on 2 mg.l-1 BAP and 0.5mg.l-1 NAA. For callus proliferation, calli were maintained on MS medium containing 2 mg.l-1 BAP, 0.1 mg.l-1 NAA and 0.5 mg.l-1 GA3. Maximum rooting percentage (70.51%) and root number (0.0.02) were obtained with 0.25 mg.l-1 NAA.


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