Effect of biotic elicitors on tropan alkaloids production in hairy roots culture of Datura (Datura innoxia)

Articles in Press

Document Type : Full Paper

Authors

1 Horticulture Biotechnology Research Group, Department of Industrial Biotechnology, Khorasan Razavi branch of Academic Center for Education, Culture and Research, Mashhad, Iran.

2 Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran.

Abstract

Hairy root culture of medicinal plants is a promising method for producing herbal medicinal compounds. This study focused on Datura innoxia, a plant known for its use in treating various diseases, with the goal of cultivating its hairy roots to produce the alkaloids hyoscyamine and scopolamine. In addition, the research aimed to explore the potential for enhancing the production of these medicinal compounds using biotic elicitors, including methyl jasmonate, yeast extract, and salicylic acid. The investigation into the effects of these different elicitors on the growth rate and alkaloid production in Datura hairy roots yielded significant findings. Treatment with methyl jasmonate did not alter the growth rate of the hairy roots but resulted in a 30% increase in alkaloid production compared to the control. On the other hand, yeast extract treatment did not change alkaloid production after 48 hours. However, the application of salicylic acid led to a notable increase in alkaloid levels. Overall, the results demonstrated that the use of elicitors is a crucial approach to enhance alkaloid production in hairy root cultures. These findings suggest that application of various elicitors in hairy root culture can be an effective strategy for maximizing the production of valuable medicinal compounds. Thus, using biotic elicitors like methyl jasmonate and salicylic acid offers a viable method to boost the yield of important alkaloids in Datura innoxia, making hairy root culture a highly effective tool in the production of medicinal plant compounds.

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Iranian Journal of Horticultural Science

Articles in Press, Accepted Manuscript
Available Online from 13 October 2024

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History

  • Receive Date: 21 November 2023
  • Revise Date: 07 July 2024
  • Accept Date: 12 July 2024

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