Document Type : Full Paper
Authors
1
Department of Horticultural Science, Faculty of Agriculture, Tarbiat Modares University (TMU), Tehran, Iran
2
Department of Horticultural Sciences, Faculty of Agricultural Engineering, University of Tarbiat modares, Tehran, Iran.
3
Department of Horticultural Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
4
Department of Horticultural Science, Faculty of Agriculture, Tarbiat Modares University, Tehran. Iran
5
Department of Plant Science, School of Biology, University of Tehran, Tehran, Iran
Abstract
In this research, effect of the number of chilling hours above zero (2 ᵒC) in five time durations (0, 700, 1400, 2100, and 2800 hours) in three replications on the dormancy and growth of tuberous roots of Kelussia odoratissima in the form of a completely randomized design was conducted. Chilling treatment including temperature 2 ᵒC was applied on the tuberous roots in time durations and some morphological and biochemical traits were measured. The highest germination rate (72%) of the tuberous root was observed in the chilling treatment of 2800 h, and in the chilling treatment of 1400 h, 50% of the tuberous roots were germinated. Chilling treatment led to acceleration of germination, increase of seedling length and stem. The highest soluble carbohydrates was recorded in the cold treatment of 2800 h (76 mg/g fresh weight) and the lowest amount was observed in the control treatment (7 mg/g fresh weight). Chilling treatment led to changes in the concentration of abscisic acid and gibberellic acid phytohormones, so that the highest concentration of gibberellic acid and the lowest amount of abscisic acid (75.5 and 20.2 nanomol per gram of fresh weight, respectively) were belonged to 2800 h treatment. In addition, chilling treatment increased the activity of alpha-amylase, beta-amylase and protease enzymes by 1.2, 0.63, and 0.35 (mg/g fresh weight), respectively. These results indicated that the chilling requirement of the K. odoratissima plant is approximately 2100 to 2800 h and the forcing culture of K. odoratissima in a controlled environment has been successful.
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