Screening of Tolerance of Plum Cultivars to the Plum Moth Grapholita funebrana (Treitschke)

Document Type : Full Paper

Authors

1 Agricultural Research, Education and Extension Organization, Horticultural Sciences Research Institute, Temperate Fruits Research Center, Karaj.Iran

2 Agricultural Research Education and Extension Organization .Horticultural Science Research Institute,Temperate Fruits Research Center, Karaj, Iran

3 Agricultural Research, Education and Extension Organization, Horticultural Sciences Research Institute, Temperate Fruits Research Center, Karaj. Iran,

Abstract

Plum fruit moth, Grapholita funebrana is one of the most important plum (Prunus domestica) pests. This project was carried from 2019 to 2021 to screen 26 cultivars and promising genotypes of plum in terms of pest larvae feeding tolerance index. The percentage of injury and the larvae population were measured every ten days until the end of October during the activity period of larvae. Then the biological stress sensitivity index was calculated. Time series models were used to investigate the correlation between injury percentage and larval population density, and the cluster analysis method was used to separate cultivars and genotypes based on tolerance index. The degree of influence of different fruit characters on tolerance was investigated by calculating Spearman's correlation coefficient. The results showed that the maximum effective day-larva index in the study years was in late July to early August. The values of cross correlation coefficient in Ghalo and Sosormi cultivars had maximum and minimum values, respectively. Finally, Queen Roza and Anjelo were the most sensitive, and Qomi, Gholaman, Faryar, G-Black, Zojlo, Gallo, G-100, G98, G99, Mortini, Black Amber and Kermanshah were the most tolerant cultivars to plum moth. Fruit surface area, sphericity index, average diameter of fruit engineering, fruit size index, fruit length and width had positive and significant correlation with tolerance indices. The results of this research are part of the breeding program of plum cultivars to develop the cultivation of this product in the country.

Keywords

Main Subjects


Extended Abstract

Introduction

    Plum fruit moth, Grapholita funebrana, is one of the most important plum (Prunus domestica) pests. The larva of this pest feeds on the flesh of the fruit. Larvae-infested fruits contain a dense mass of black excrement, which greatly reduces the economic value of the product. The damage rate of this pest is reported 30 to 70 percent. The advantages of using resistant plum cultivars can be mentioned by reducing the cost of purchasing pest control inputs and increasing yield per unit area. Consequently, the introduction of plum cultivars tolerant to plum moth is an important and necessary step in the good management of this pest in the country.

 

Materials and methods

This project was carried out in the collection garden of the temperate and cold-season fruit research institute located in the city of Kamalshahr, Karaj, from 2019 to 2021.To carry out this project, 26 promising plum cultivars and genotypes named Kh-Mashhad, Sosormi, G-balck, G98, Mortini, Anjelo, Songhor abadi, Ghomi, Kermanshah, Black Amber, G99, Geontype 19, Feriyar, Zard Kordestane, Black star, Ghalo, Bokhara, Queen roza, Laroda, Gr-Rezaeyeh, Gholaman, Zojelo, Uromieh 20, Santarza, G-Malayer and G100 were used. Estimation of the percentage of infection with plum larvae was done at the peak of its activity, i.e., in the middle of May, every ten days until the end of October.Twenty randomly selected fruits from four trees of each genotype in four directions (south, north, east and west) of each tree were harvested and transferred to the laboratory. The fruits were chopped and the number of healthy and infected fruits was counted on the basis of the presence or absence of larvae or their remnants. The number of active larvae inside the fruits was counted separately. After estimating the injury percentage and the effective larval population, strees susceptibility, stress tolerance, damage and tolerance indices, were also calculated for each cultivar. To examine the quantitative and qualitative characteristics of the fruit, 10 ripe fruits from each tree were transferred to the laboratory. Time series models were utilized to study the correlation between damage percentage and larval population. The cluster analysis method was used to classify cultivars and genotypes based on the tolerance indices. The degree of the effect of different fruit characteristics on the tolerance level was investigated by calculating the correlation coefficient using the IBM® SPSS 27.0.1 IF026 software.

 

Results and Discussion

The damaging population of the pest started its activity from the second half of May and gradually the density of the active larvae population on the plum fruit increased. The maximum effective day-larva index in the studied years was in late July to early August. Although this pattern had the same trend in most of the studied cultivars, differences were also observed in some of them. So that in Anjelo, Gholaman, Kh-Kermanshah and Zojelo cultivars the maximum activity of pest larvae was happened with a little delay, in late August to early September, while in some cultivars such as Kermanshah it happened earlier than other cultivars, in late June. In three years of study, Ghalo and Sosormi cultivars have, respectively, the maximum and minimum values of the cross correlation coefficient between the percentage of damage and the population of harmful larvae. Genotypes with toletrance reactions included Gholaman, Faryar, G-Black, Zojelo, Gallo, G-100, Qomi, G98, G99, Mortini, Black Amber and Kermanshah. Among the investigated characters, fruit surface area, sphericity index, average diameter of fruit engineering, fruit size index, fruit length, and fruit width had a positive and significant correlation with tolerance indices. The important point is the extent that defensive traits provide sustainable pest control. Adaptation and selection of defense traits with different types of pests to optimize the breeding process of tolerant cultivars depends on the nature of the damage caused by the pest, whether it is direct feeding damage, visual spoilage, or whether the pest is a disease carrier. Focusing on specific traits that make plants resistant to pests is vital for the development of pest-tolerant fruit tree germplasm.

 

Conclusion

In general, the germplasms of Queen Rosa and Angelo were the most sensitive, and Qomi, Gholaman, Faryar, G-Black, Zoglu, Gallo, G-100, G98, G99, Moretini, Black Amber and Kermanshah were the most tolerant cultivars to plum moth. An advantage of using tolerant cultivars as part of integrated pest management is ecological compatibility with other control tactics. One of the most important tasks for breeders is to find and use the natural diversity of plant defense characters and transfer them to future cultivars to improve the inherent resistance of horticultural products, including plum, against pests. The results of this research are also a part of the breeding program of plum cultivars to develop the cultivation of this product in the country.

Abebe, W. (2021). Review on plant defense mechanisms against insect pests. International Journal of novel research in interdisciplinary studies, 8, 15-39.
Al-Hooti, S., Sidhu, J. S., & Qabazard, H. (1997). Physicochemical characteristics of five date fruit cultivars grown in the United Arab Emirates. Plant Foods for Human Nutrition, 50(2), 101-113.
Alphey, N. & Bonsall, M. B. (2018). Genetics based methods for agricultural insect pest management. Agricultural and forest entomology, 20(2), 131-140.
Beltrán Sanahuja, A., Maestre Pérez, S. E., Grané Teruel, N., Valdés García, A. & Prats Moya, M. S. (2021). Variability of chemical profile in almonds (Prunus dulcis) of different cultivars and origins. Foods, 10(1), 153.
Cai, C. J., Ma, Z. H., Wang, H. G., Zhang, Y. P. & Huang, W. J. (2007). Comparison research of hyperspectral properties between near-ground and high altitude of wheat stripe rust. Acta Phytopathologica Sinica, 37(1), 77-82.
Farshadfar, E., Poursiahbidi, M. M. & Safavi, S. M. (2013). Assessment of drought tolerance in land races of bread wheat based on resistance/tolerance indices. International Journal of Advanced Biological and Biomedical Research, 1(2), 143-158.
Fischer, R. A. & Maurer, R. (1978). Drought resistance in spring wheat cultivars. I. Grain yield responses. Australian Journal of Agricultural Research, 29(5), 897-912.
Frades, I. & Matthiesen, R. )2010(. Overview on techniques in cluster analysis. Bioinformatics methods in clinical research, 81-107.
Fraley, C. & Raftery, A. E. (2002). Model-based clustering, discriminant analysis, and density estimation. Journal of the American statistical Association, 97(458), 611-631.
Głowacka, A, & Rozpara, E. (2014). Growth, yielding and fruit quality of four plum (Prunus domestica L.) cultivars under organic orchard conditions. Journal of Research and Applications in Agricultural Engineering, 59(3).
Gogtay, N. J. & Thatte, U. M. )2017(. Principles of correlation analysis. Journal of the Association of Physicians of India, 65(3), 78-81.
Gonzalez Guzman, M., Cellini, F., Fotopoulos, V., Balestrini, R. & Arbona, V. (2022). New approaches to improve crop tolerance to biotic and abiotic stresses. Physiologia Plantarum, 174(1), e13547.
González‐Tokman, D., Córdoba‐Aguilar, A., Dáttilo, W., Lira‐Noriega, A., Sánchez‐Guillén, R. A, & Villalobos, F. (2020). Insect responses to heat: physiological mechanisms, evolution and ecological implications in a warming world. Biological Reviews, 95(3), 802-821.
Hamzehzarghani, H., Kushalappa, A. C., Dion, Y., Rioux, S., Comeau, A., Yaylayan, V., ... & Mather, D. E. (2005). Metabolic profiling and factor analysis to discriminate quantitative resistance in wheat cultivars against fusarium head blight. Physiological and Molecular Plant Pathology, 66(4), 119-133.
Hossain, A. B. S., Sears, R. G., Cox, T. S. & Paulsen, G. M. (1990). Desiccation tolerance and its relationship to assimilate partitioning in winter wheat. Crop Science, 30(3), 622-627.
Javed, H., Mohsin, A., Aslam, M., Naeem, M., Amjad, M. & Mahmood, T. (2011). Relationship between morphological characters of different aubergine cultivars and fruit infestation by Leucinodes orbonalis Guenee. Pakistan Journal of Botany, 43(4), 2023-2028.
Joshi, N.K., Rajotte, E.G., Myers, C.T., Krawczyk, G. & Hull, L.A. (2015). Development of a susceptibility index of apple cultivars for codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae) oviposition. Frontiers in Plant Science, 6, 992.
Kanga, L. H., Pree, D. J., Van Lier, J. L., & Walker, G. M. (2003). Management of insecticide resistance in oriental fruit moth (Grapholita molesta; Lepidoptera: Tortricidae) populations from Ontario. Pest Management Science: formerly Pesticide Science, 59(8), 921-927.
Karmakar, P., Pal, S., & Chakraborty, G. (2021). Effects of cabbage cultivars on the food consumption and utilization parameters of diamondback moth, Plutella xylostella (L.). International Journal of Tropical Insect Science, 1-10.
Khayatnezhad, M. & Gholamin, R. (2020). A modern equation for determining the dry-spell resistance of crops to identify suitable seeds for the breeding program using modified stress tolerance index (MSTI). Bioscience Biotechnology Research Communications, 13(4), 2114-2117.
Koch, K. G., Chapman, K., Louis, J., Heng-Moss, T., & Sarath, G. (2016). Plant tolerance: a unique approach to control hemipteran pests. Frontiers in plant science, 7, 1363.
Kumari, P., Jasrotia, P., Kumar, D., Kashyap, P. L., Kumar, S., Mishra, C. N., Kumar, S. & Singh, G. P. (2022). Biotechnological approaches for host plant resistance to insect pests. Frontiers in Genetics, 13, 914029.
Li, H., Futch, S. H. & Syvertsen, J. P. (2007). Cross‐correlation patterns of air and soil temperatures, rainfall and Diaprepes abbreviatus root weevil in citrus. Pest Management Science: formerly Pesticide Science, 63(11), 1116-1123.
Little T.J., Shuker D.M., Colegrave N., Day, T.  & Graham, A. L. (2010). The coevolution of virulence: tolerance in perspective. PLoS Pathogens, 6: e1001006.
Lu, P.F., Huang, L.Q. & Wang, C.Z. (2012). Identification and field evaluation of pear fruit volatiles attractive to the oriental fruit moth, Cydia molesta Journal of Chemical Ecology, 38, 1003–1016.
Machlitt, D .(1998). Persea mite on avocados: quick field counting method. Subtropical Fruit, 6, 1–4.
Markheiser, A., Rid, M., Biancu, S., Gross, J. & Hoffmann, C. (2018). Physical factors influencing the oviposition behaviour of European grapevine moths Lobesia botrana and Eupoecilia ambiguellaJournal of Applied Entomology, 142, 201–210.
Mitrea, I. & Bancă, G. (2011). Behavior of some plum varieties to the attack of the plum moth Grapholitha funebrana Tr. Lucrări Științifice-Universitatea de Științe Agronomice și Medicină Veterinară București. Seria B, Horticultură ,55, 410-413.
Moreau, J., Benrey, B. & Thiery, D. (2006). Grape variety affects larval performance and also female reproductive performance of the European grapevine moth Lobesia botrana (Lepidoptera: Tortricidae). Bulletin of Entomological Research,.  96, 205–212.
Moreau, J., Thiéry, D., Troussard, J.P. & Benrey, B. (2007). Grape variety affects female but also male reproductive success in wild European grapevine moths. Ecological Entomology, 32, 747–753.
Mulwa, G. K., Kitonyo, O. M. & Nderitu, J. H. (2023). Earliness and crop morphological traits modulate field pest infestation in green gram. Journal of Economic Entomology. toac205.
Myers, C.T. Hull, L.A. & Krawczyk, G. (2006). Seasonal and cultivar associated variation in oviposition preference of oriental fruit moth (Lepitoptera: Tortricidae) adults and feeding behavior of neonate larvae in apples. Journal of Economic Entomology, 99, 349–358.
Pavan, F., Stefanelli, G., Villani, A. & Cargnus, E. (2018). Influence of grapevine cultivar on the second generations of Lobesia botrana and Eupoecilia ambiguellaInsects, 9, 8.
Peterson, R. K., Varella, A. C. & Higley, L. G. (2017). Tolerance: the forgotten child of plant resistance. PeerJ, 5, e3934.
Pluciennik, Z., Tworkowska, U. & Omiecinska, B. (1999). Preference of plum fruit moth (Laspeyresia funebrana Tr.) to some plum cultivars. Journal of Fruit and Ornamental Plant Research (Poland),
Rauleder, H. (2002). Observations on the biology of the plum fruit moth (Cydia funebrana). Gesunde Pflanzen, 54(8), 241-248.
Renwick, J.A.A. (1989). Chemical ecology of oviposition in phytophagous insects. Experimentia, 45, 223–228.
Rosenthal, J. P. & Kotanen, P. M. (1994). Terrestrial plant tolerance to herbivory. Trends in Ecology & Evolution, 9(4), 145-148.
Sabzi, S., Nadimi, M., Abbaspour-Gilandeh, Y. & Paliwal, J. (2022). Non-destructive estimation of physicochemical properties and detection of ripeness level of apples using machine vision. International Journal of Fruit Science, 22(1), 628-645.
Sarker, S. & Lim, U. T. (2019). Development and fecundity performance of Grapholita molesta and Grapholita dimorpha (Lepidoptera: Tortricidae) on different immature fruits. PLoS One, 14(5), e0217492.
Sharon, R.; Zahavi, T. Soroker, V. & Harari, A.R. (2009). The effect of grape vine cultivars on Lobesia botrana (Lepidoptera: Tortricidae) population levels. Journal of Pest Science,  82, 187–193.
Stout, M. & Davis, J. (2009). Keys to the increased use of host plant resistance in integrated pest management. In Peshin, R. & Dhawan, A. K. Integrated Pest Management: Innovation- Development Process. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8992-3_7.
Thiéry, D. & Moreau, J. (2006). Grape cultivar affects larval and female fitness of the European grapevine moth, Lobesia botrana (Lepidoptera: Tortricidae). IOBC-WPRS Bulleti,.  29, 131–138.
Timm A.E., warnich L. & H. geerTseMa (2008). Morphological and molecular identication of economically important Tortricidae (Lepidoptera) on deciduous fruit tree crops in South Africa.  African Entomology, 16, 209-219.
Torriani, M.V., Mazzi, D., Hein, S. & Dorn, S. (2010). Structured populations of the oriental fruit moth in an agricultural ecosystem. Molecular Ecology, 19, 2651–2660.
Wearing, C.H. (2016). Distribution characteristics of eggs and neonate larvae of codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae). International Journal of Insect Science, 8, IJIS-S38587.
Whitehead, S.R., Turcotte, M.M. & Poveda, K. (2017). Domestication impacts on plant–herbivore interactions: a meta-analysis. Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, 372, 0160034.
Winkler, A., Athoo, T. & Knoche, M. (2022). Russeting of fruits: Etiology and management. Horticulturae, 8(3), 231.
Woodcock, P., Marzano, M. & Quine, C.P. (2019). Key lessons from resistant tree breeding programmes in the Northern Hemisphere. Annals of Forest Science, 76, 51.