Document Type : Full Paper
Authors
1
Doctoral student of Department of Horticultural Sciences, Faculty of Agriculture, Mohaghegh Ardabili University, Ardabil, Iran.
2
Professor, Department of Horticultural Sciences, Faculty of Agriculture, Mohaghegh Ardabili University, Ardabil, Iran.
3
Assistant Professor, Department of Horticultural Sciences, Faculty of Agriculture, Mohaghegh Ardabili University, Ardabil, Iran.
4
4- Professor, Department of Horticultural Sciences, Faculty of Agriculture, Mohaghegh Ardabili University, Ardabil, Iran.
Abstract
Iris pseudacorus L. is one of the native and prominent ornamental species. The main problem in starting tissue culture of this plant is the presence of severe fungal and bacterial infections, especially in the rhizome of this plant, which causes the failure of conventional sterilization methods. The present study was performed in order to optimize the best and least dangerous disinfection method suitable for in vitro culture of this plant with the help of rhizomes and seeds. For this purpose, in two separate experiments in a completely randomized design, 16 types of rhizome treatments and 15 types of seed treatments were performed in 5 replications for sterilization operations. The treated specimens were cultured in hormone-free MS medium after disinfection. Two weeks after planting, various indices such as infection percentage, germination percentage, mean leaf length and mean root length were measured. The results showed thatA15 treatment of rhizome explants with the help of spa bath and fluconazole fungicide and antibiotics streptamycin and alcohol70% and NaoCl4%, the lowest contamination percentage(11%) and the germination rate of 88% was the best treatment. Also, A13 treatment with 85% germination and 15% contamination was the second good treatment. In seed samples, treatment B2 using hot water and 3% sodium hypochlorite and treatment B5 using hydropriming with running water and 2% hypochlorite had the highest germination rate of 71.74% and contamination was almost zero. The results of processing of sterilized samples showed that the concentration of 5micromolarTDZ+6micromolar of NAA hormone resulted in the highest amount of processing.
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