The Effect of Calcium in the Fruit Puffiness Reduction of Satsuma Mandarin (Citrus unshiu 'Sugiyama')

Document Type : Full Paper

Authors

1 Department of Soil and Water Research Department, Mazandaran Agricultuarl and Nutural Resources Research and Education, AREEO, Sari, Iran.

2 Agronomy and Horticultural Science Department, Mazandaran Agricultuarl and Nutural Resources Research and Education, AREEO, Sari, Iran.

Abstract

In order to investigate the fruit puffiness status and the effect of calcium nitrate spray on quality and puffiness of Satsuma mandarin fruit, two separate experiments were conducted. In the first experiment, the puffing rate of Satsuma mandarin (Citrus unshiu 'Sugiyama') fruit in different orchards in the eastern region of Mazandaran was compared. In the second experiment, the effect of calcium nitrate application as foliar spray on reduction of fruit puffines was examined. The experiment was performed in a commercial orchard, with sour orange rootstock, based on a randomized complete block design, with six treatments and four replications. Treatments included: T1. Control (no Calcium nitrate spray); T2. Calcium nitrate spray in late May; T3. Calcium nitrate spray in mid- June; T4. Calcium nitrate spray in early July; T5. Calcium nitrate spray in late May and mid- June; T6. Calcium nitrate spray in late May, mid- June and early July. The results of the first experiment showed that the percentage of puffed fruits in the orchards of different regions varied from 27 to 46%, and the number of puffed fruits increased significantly from the early of November. The results obtained from the second experiment indicated that calcium nitrate spray in T5 (late May and mid- June) and T6 (late May, mid- June and early July) treatments had a significant effect on puffiness, so that the number of puffy fruits reduced by about 20% compared to the control, but this anomaly did not completely prevented. Also, calcium nitrate spray had a significant effect on Ca concentration in fruits. The highest concentration of Ca in whole fruit, fruit peel and inner skin (albedo) occurred in T5 and T6 treatments. Therefore, calcium nitrate spray is recommended at least 2 times (late May and mid- June) up to a maximum of 3 times (late May, mid- June and early July) in Satsuma mandarin Orchards.

Keywords

Main Subjects


Extended Abstract

Introduction

The citrus cultivation area in Mazandaran province, north of Iran, is about 120000 ha with about 3 million tons of annual production. Mandarin is one of the most important citrus varieties in this region and its cultivation area in Mazandaran province is about 25000 ha, with annual production about 500000 tons per year. One of the most important physiological abnormalities in the Satsuma mandarin cultivar is puffiness, which is a serious problem in the citrus industry, especially in the old orchards of the north region of Iran. These puffed fruits are easily damaged during the process of harvesting, sorting, packing, transportation, and storage. Therefore, the puffy fruits are not desirable in the market. Application of calcium is known to reduce peel puffiness to some extent. Calcium penetrates into the epidermis of the peel cells and is then quickly absorbed by lower epidermis. This reduces or prevents the activity of enzymes that cause peel puffiness. Therefore, the peel Ca content can have a great impact on reducing physiological disorders, especially fruit puffiness, and increasing the net profit of producers.

  

Materials and methods

In order to investigate the fruit puffiness status and the effect of calcium nitrate spray on quality and puffiness of  Satsuma mandarin fruit, two separate experiments were conducted. In the first experiment, the rate of Satsuma mandarin (Citrus unshiu 'Sugiyama') fruit puffiness in different orchards, in the eastern region of Mazandaran was compared. In the second experiment, the effect of calcium nitrate application as foliar spray on reduction of fruit puffines was examined. The experiment was performed in a commercial orchard, with sour orange rootstock, based on a randomized complete block design, with six treatments and four replications. Treatments included: T1. Control (no calcium nitrate spray); T2. Calcium nitrate spray in late May; T3. Calcium nitrate Spray in mid- June; T4. Calcium nitrate spray in early July; T5. Calcium nitrate spray in late May and mid- June; T6. Calcium nitrate spray in late May, mid- June and early July.

 

Results and discussion

The results of the first experiment showed that the percentage of puffed fruits in the orchards of different regions varied from 27 to 46%, and the number of puffed fruits increased significantly from the early of November. The results obtained from the second experiment indicated that calcium nitratespray in T5 (late May and mid- June) and T6 (late May, mid- June and early July) treatments had a significant effect on puffiness, so that the number of puffy fruits reduced by about 20% compared to the control, but this anomaly did not completely prevented . Also, calcium nitrate spray had a significant effect on fruit Ca concentration. The highest concentration of Ca in whole fruit, fruit peel and inner skin (albedo) occurred in T5 and T6 treatments. Therefore, calcium nitrate spray is recommended at least twice (late May and mid- June) up to three times (late May, mid- June and early July) in Satsuma mandarin orchards. The peel puffiness is not only associated with changes in albedo cells, but also by significant changes in the cells of lower epidermis. Using calcium nitrate foliar application allows calcium to penetrate directly into the fruit through the cuticle. This suggests that the mechanism that controls the calcium is more physical than physiological. In fact, it keeps the stomata open and facilitates the transpiration. Fruit puffiness is not completely controlled by calcium application, but it is significantly reduced to an economic level. Developing tissues have an urgent and continuous need for calcium. This calcium is supplied by xylem vessels and depends strongly on transpiration rate from the surface of that organ. Calcium deficiency and the resulting disorders occur when low transpiration and the flow of raw sap cannot provide enough calcium for the development of those tissues. In different regions of Mazandaran province, the number of sunny hours is low, especially in the first stage of fruit growth, and the relative humidity is high. Therefore, there is a possibility of calcium deficiency in most years, especially in tissues that have low transpiration. Calcium deficiency in the fruitlets development stage reduces the cellular communication between peel cells and increases the possibility of puffiness. The maximum absorption of calcium in citrus fruits is approximately from after fruit set to 45-50 days after flowering. After June drop, calcium absorption decreases rapidly. In citrus fruits, most of the calcium enters the albedo tissue in the first phase of fruit growth until about 100 days after flowering; then the calcium is evenly distributed between the albedo and the pulp. In the case of citrus fruits, after the physiological drop, the wax of fruit peel surface develops rapidly. The increase of wax will reduce the efficiency of calcium absorption.

 

Conclusion

In general, the percentage of puffed fruits in the orchards of different regions varied from 27 to 46%, and the number of puffed fruits increased significantly from early November. Also, spraying calcium nitrate in the first stage of fruit growth (after fruit set to about 20 to 30 days after the physiological drop of the fruits) caused an increase in calcium content of the fruit peel, especially in the inner skin (Albedo), and reduced the rate of puffing fruits. Therefore, based on the results of this research, it is recommended to spray calcium nitrate 2 -3 times, with intervals of approximately 15 days, from late May to early July.

Agusti, M., Almela, V., Zaragoza, S., Gazzola, R. & Primo-Millo, E. (1997). Alleviation of peel-pitting of fortune mandarin by the polyterpene pinolene. Journal of Horticultural Science, 72, 653-658.
Ahmadi, K., Ebadzadeh, H., Hatami, F., Hosseinpour, R., & Abdshah, H. (2018). Agricultural statistics (horticultural products). Information and Communication Technology Center, Ministry of Agricultural Jihad, Tehran, Iran. (In Persian)
Akhlaghi Amiri, N & Asadi Kangarshahi, A. (2015). Possibility of reducing Satsuma mandarin (Citrus unshiu) fruit puffing in East of Mazandaran, Iranian Journal of Horticultural Science, 46(4), 590-600. (In Persian)
Asadi Kangarshahi, A. & Akhlaghi Amiri, N. (2014 a). Advanced and Applied Citrus Nutrition (1st. ed.). Agricultural Extension and Education Publications. (In Persian)
Asadi Kangarshahi, A. & Akhlaghi Amiri, N. (2014 b). Advanced and Applied Citrus Nutrition (2nd. ed.). Agricultural Extension and Education Publications. (In Persian)
Asadi Kangarshahi, A. & Akhlaghi Amiri, N. (2021). Effect of urea spray accordance with growth phenology on yield and alternate bearing of Satsuma mandarin (Citrus unshiu). Iranian Journal of Horticultural Science, 52(1), 99-111. (In Persian)
Asadi Kangarshahi, A. (2019). Nutition Management of Citrus Trees (1st. ed). Agricultural Extension and Education Publications. (In Persian)
Awasthi, R.P., & Nauriyal, J.P. (1972). Studies on granulation in sweet orange. VI. Differences in moisture, total soluble solids and ascorbic acid of juice vesicles in different stages of granulation. Punjab Horticultural Journal, 12, 203– 211.
Bashour, I. & Sayegh, A.A. (2007). Methods of Analysis for Soils of Arid and Semi-Arid Regions. 49-53. Food and Agriculture Organization of the United Nations, Rome.
Bramlage, W.J. (1994). Physiological Role of Calcium in Fruit. In Tree Fruit Nutrition. Petersen, A.B. & Stevens, R.G. (eds), Good Fruit Grower, Yakima, WA. 101-107.
Burdon, J., Lallu, N., & Yearsley, C. (2007). Postharvest conditioning of satsuma mandarins for reduction of acidity and skin puffiness. Postharvest Biology and Technology, 43, 102–114.
Chen, X. & Zhang, B. (1988). Studies on the‘Hong-Ju’ puffing fruits. Acta Horticulturae Sinica, 15 (1), 13-17.
Davies, F.S., & Alberigo, L.G. (1994). Citrus. CAB International, Wallingford UK.
Emami, A. (1996). Methods of plant analysis. Journal of Soil and Water Research Institute, 2 (982) 128-128.. (In Persian)
Epstein, E. & Bloom, A. (2005). Mineral nutrition of plants: Principles and perspectives. 2nd ed. Sinauer associates Inc, Plumtree Road, Sunderland.
Grierson, W. (1981). Physiological disorders of citrus fruits. Proc. Int. Soc. Citriculture. 3, 764-767.
Hardy, S. & Sanderson, G. (2010). Citrus Maturity Testing. Department of Industry and Investment, State of New South Wales, Australia: 1-6. http://www.dpi.nsw.gov.au/__data/assets/pdf_file/0020/3 20294/Citrus
Hirschi, K.D. (2004). The calcium conundrum. Both versatile nutrient and specific signal. Plant Physiology. 136, 2438-2442.
Hopkins, W.G. & Huner, N.P.A. (2004). Plant and Inorganic Nutrient. In Introduction to Plant Physiology. 3nd ed. John Wiley and Sons. Inc. Publishers. 241-257.
Iglesias, D.J., Cercos, M., Colmenero-Flores, J.M., Naranjo, M.A., Rios, G., Carrera, E., Ruiz-River, O., Liso, I., Morillon, R., Tadeo F.R., & Talon, M. (2007). Physiology of citrus fruiting. Brazilian Journal Plant Physiology, 9, 333-362.
Jiang, Y., Liu, S., & Chen, F. (1991). Low water and control of postharvest fruit. Bulletin of Botany, 8 (2): 9-12.
Kawase, K. & Hirai, M. (1983). Growth, sugar accumulation and puffiness of the mandarin peel during coloring. Journal of Japanese Society of Horticultural Science, 52, 231-237.
Kawase, K., Suzuki, K. & Hirose, K. (1981). Use of growth regulators to control rind puffing of Satsuma mandarin fruit. Proceeding of the International Society of Citriculture: 237-239.
Kimball, D.A. (1991). Citrus Processing: quality control and technology. Springer Science, New York.
Kuraoka, T., Iwasaki, K. & Kadoya, K. (1966). Studies on peel puffing and levels of GA like substances and ABA in the peel of Satsuma mandarin (Citrus unshiu). In: Proceeding of Japanese Society of Horticultural Science, 43-44.
Kuraoka, T., Iwasaki, K., Hino, A. & Tsugi, H. (1975a). Studies on the peel puffing of the Satsuma mandarin III. The conversion of pectic substances and calcium distribution within the peel. Journal of Japanese Society of Horticultural Science, 44, 15-21. (In Japanese)
Kuraoka, T., Iwasaki, K., Hino, A. & Tsugi, H. (1975b). Studies on the peel puffing of the Satsuma mandarin II. Change of cell morphology and cellulose activity during the development of the fruit rind. Journal of Japanese Society of Horticultural Science, 44, 7-14. .(In Japanese)
Marschner, H. (1995). Mineral nutrition of higher plants. 2nd Edition Academic Press, San Diego.
Masaya, S., Pear, M., Yoshitaka, M., Masakazu, F. & Touru, M. (1999). Effects of calcium compounds on fruit puffing and the ultrastructural characteristics of the subepidermal cell walls of puffy and calcium-induced non-puffy satsuma mandarin fruits. Journal of the Japanese Society for Horticultural Science, 68 (5), 919–926. .(In Japanese)
Mhadhvi, J. (2012). Investigation of phenological stages of Thomson navel orange and miagava and sugiyama Satsuma mandarin in foothills, plain and shoreline area in Sari city. Master degree thesis, Department of Horticultural Sciences, Islamic Azad University – Karaj, Iran. (In Persian)
Nagy, S., Marshall, M., Wardowski, W.F. & Rouseff, R.L. (1985). Postharvest creasing of Robinson tangerines as affected by harvest date and pectin esterase activity and calcium content. Journal of Horticultural Science, 60, 137-140.
Peryea, F.J. (1991). Preharvest calcium spray evaluation. In: A.B. Petersen, R.G. Stevens (eds) Tree fruit nutrition. Good fruit grower, Yakima, WA. 123-124
Roberts, T.L. (2008, Feb.) Improving nutrient use efficiency. IFA Agriculture Conference. Kunming China.
Saure, C.M. (2004). Calcium translocation to fleshy fruit: its mechanism and endogenous control. Scientia Horticulture, 105, 65-89.
Shah Beig, M.A. (1997). Reducing postharvest losses in citrus fruits by cooling, heat therapy, PE coating and modified atmosphere. Articles for Word Food Day, Information and Communication Technology Center, Ministry of Agricultural Jihad, Tehran, Iran. (In Persian)
Shi, X., & Shen, G. (2003). Occurrence and prevention of pumice peel of Wenzhou mandarin orange fruit. Zhejiang Citrus, 20 (2), 17-18.
Shi, X., Xu, J., Zhang, L., Fan, G., & Lin, M. (2008). The relationship between the puffiness of Wenzhou mandarin orange fruit and its storage. Journal of Zhejiang Agricultural Sciences, 20 (4), 304–307.
Shi, X., Xu, J., Zhang, L., Fan, G. & Lin, M. (2010). Studies on the peel puffiness and storage of mature fruit in Satsuma mandarin. In: Proceedings of 11th International Citrus Congress, Wuhan, China, 912-914.
Shiraishi, M., Mohammad, P., Makita, Y., Fujibuchi, M. & Manabe, T. (1999). Effects of calcium compounds on fruit puffing and the ultrastructural characteristics of the sub epidermal cell walls of puffy and calcium-induced non-puffy Satsuma mandarin fruits. Journal of the Japanese Society for Horticultural Science, 68, 919-926.
Storey, R. & Treeby, M.T. (2002). Nutrient uptake into navel oranges during fruit development. Journal of Horticultural Science Biotechnology, 77, 91-99.
Sun, J., Chen, J., Shi, X., Xu, H., Pang, Q., Zhang, L., & Xie, M. (2011). The effect of compound nutrient solution treatment on the fruit quality and floating skin of the fully harvested 'Gongchuan' Wenzhou mandarin orange. Zhejiang Journal of Agriculture, 23 (2), 283 – 287.
Taiz, L. & Zeiger, E. (2002). Mineral nutrition, P. 67-86. Plant Physiology. 3rd ed. Sinauer Associates Inc. Publishers.
White, P.J. & Broadley, M.R. (2003). Calcium in plants. Annals of Botany. 92, 487-511.
Williamson, G. (1991). Purification and characterization of pectin acetylesterase from orange peel. Phytochemistry, 30, 445-449.
Yan S. (2013). Puffiness prevention-control of citrus fruits. Hunan Agriculture, (3), 16-25.
Zaragoza, S., Almela, V., Tadeo, F.R.  Primo-Millo, E. & Agusti, M. (1996a). Effectiveness of calcium nitrate and GA3 on the control of peel-pitting of fortune mandarin. Journal of Horticultural Science, 71, 321-326.
Zaragoza, S., Gazzola, R., Trenor, I., Alonso, E., Primo-Millo, E., Almela, V. Juan, M. & Agusti, M. (1996b). Control of peel-pitting of fortune mandarin. Proceedings of Internatoinal Society Citriculture, 2, 1105-1109.
Zhang, Q., Wei, Y., Zheng, Y. (2001). Mechanism of puffiness and the methods of control in citrus fruit.Ⅱ: relation between puffiness and senescence of the early-maturing satsuma mandarin. Journal of Hunan Agricultural University, Natural Sciences, 27 (1), 32–34.
Zong R., Shao, P., & Hu, X. (1979). The preliminary discussion of juice sacs granulation and citrus peel growth and decline of citrus fruit. Scientia Agricultura Sinica, 3, 60– 64.