Iranian Journal of Horticultural Science

Iranian Journal of Horticultural Science

Effects of application of GABA and calcium lactate on photosynthesis and nitrate ‎accumulation in Lactuca sativa ‘Dimayo’ ‎

Document Type : Full Paper

Authors
Najme Zeinalipour 1
Fatemeh Aghebati 2
1 Assistant Professor, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
2 M. Sc. Student, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
10.22059/ijhs.2022.337264.1997
Abstract
Nitrate uptake in plants is very important due to environmental concerns and the quality of agricultural products. On the other hand, photosynthetic efficiency is considered as the principal indicator of plant growth and yield, therefore the ability to sustain crop stability in different environmental conditions is crucial. In this study, some physiological characteristics and nitrate accumulation were investigated using γ-aminobutyric acid (GABA) and calcium lactate in lettuce of Dimayo cultivar. For this purpose, GABA solutions with three concentrations (0, 0.5 and 1.5 mg/l) and calcium lactate with three concentrations (0, 1 and 2 %) and their interaction were used, as foliar spraying during two times including after seedling transplant and two weeks later. This factorial experiment was performed in a completely randomized design in the greenhouse. GABA treatment of 1.5 mg/l + calcium lactate 2% showed the highest yield, total chlorophyll and relative water content. However, the highest ion leakage and nitrate accumulation were obtained in the leaves of control plants. Also, the lowest amount of nitrate accumulation was measured in GABA treatment with a concentration of 1.5 mg/l, which reduced the amount of nitrate accumulation compared to control plants by four times.  
Keywords
Chlorophyll
iceberg
ion leakage
relative water content‎
Subjects
  1. Afsari Yeganeh, S., Golchin, A., Delshad, M. & Abdossi, V. (2022). Effect of zinc enrichment of agricultural waste compost on yield and some qualitative characteristics of lettuce in greenhouse cultivation (Lactuca sativa). Iranian Journal of Horticultural Science, 53(3), 707-720. (In Farsi).
  2. Alexander, J., Benford, D., Cockburn, A., Cravedi, P.,Dogliotti, E. & Domenico, A. (2008). Nitrate in vegetables, scientific opinion of the panel on contaminantsthe food chain. European Food Safety Authority Journal, 9(1), 68-79.
  3. Ashrafuzzaman, M., Ismail, M.R., Fazal, K.M.A.I., Uddin, M.K. & Prodhan, A.K.M.A. (2010). Effect of GABA application on the growth and yield of bitter gourd (Momordica charantia). International Journal of Agriculture and Biology, 12(1), 129-132.
  4. Barbarosa, J.M., Singh, N.K., Cherry, J.H. & Locy, R.D. (2010). Nitrate uptake and utilization is modulated byexogenous γ-aminobutyric acid in Arabidopsis thaliana Journal of Plant Physiology and Biochemistry, 48(2), 443-450.
  5. Bouche, N. & Fromm, H. (2004). GABA in plants: Just a metabolite. Trends in Plant Science, 9(3) 110-115.
  6. Coronel, G., Chang, M. & Rodríguez-Delfín, A. (2008). Nitrate reductase activity and chlorophyll content in lettuce plants grown hydroponically and organically. In: Proceedings of 3th International Symposium on Soilless Culture and Hydroponics, 19-22 Mar., Cyprus, Lemesos, pp. 137-144.
  7. Dong, C.X., Zhou, J.M., Fan, X.H. & Wang, H.Y. (2004). Application methods of calcium supplements affectnutrient levels and calcium forms in mature tomato fruits. Journal of Plant Nutrition, 27(5), 1443-1455.
  8. Dordas, C. (2009). Foliar application of calcium and magnesium improves growth, yield,and essential oil yield of oregano (Origanum vulgare ssp. hirtum). Industrial Crops and Products, 29(2), 599-608.
  9. Gong, R. J., Lu, F. G., Xia, Q. P., Zhang, G. H., Yang, L. W. & Gao, H. B. (2012). Effectsof exogenous γ-aminobutyric acid on nitrate content and quality in lettuce. Journal of Agricultural University of Hebei, 3(7), 142-153.
  10. Haft Baradaran, Sh., Malakouti, M.J. & Khoshgoftarmanesh, A.H. (2018). Investigation of nitrate risk assessment in edible parts of some crops grown in Isfahan province. Applied Soil Research, 6(1),1-12. (In Farsi).
  11. Hasani Moghadam, E., Bazdar, A. & Shaban, M. (2019). Study of nitrate rate in some vegetables cultivated in Poldokhtar and Khorramabad, Lorestan province. Iranian Journal of Health and Environment, 12(1), 101-112. (In Farsi).
  12. Heidari, N., Pouryusef, M. & Tavakoli, A. (2015). Effects of drought stress on photosynthesis, its parameters and relative water content of anise (Pimpinella anisum ). Journal of Plant Research (Iranian Journal of Biology), 27(5), 829-839. (In Farsi).
  13. Heydari, S., Soltani, F., Azizi, M. & Hadian, J. (2014). Foliar application of Ca and K improves growth, yield, essential oil yield and nutrient uptake of tarragon (Artemisia dracunculus) grown in Iran. International Journal of Biosciences, 4(12), 323-338. (In Farsi).
  14. Islam, M.R., Prodhan, A.K.M.A., Islam, M.O. & Uddin, M.K. (2010). Effect of plant growth regulator (GABA) on morphological characters and yield of black gram (Vigna mungo). Journal Agriculture Research, 48(1), 73-80.
  15. Jakab, G., Ton, J., Flors, V., Metraux, J.P. & Mauch-Mani, B. (2005).Enhancing Arabidopsis salt and drought stress tolerance by chemical priming for its abscisic acid responses. Journal of Plant Physiology, 139(1), 267-274.
  16. Jia, Y., Zou, D., Wang, J., Sha, H., Liu, H., Inayat, M. A. & Zhao, H. (2017). Effects of γ-aminobutyric acid, glutamic acid, and calcium chloride on rice (Oryza sativa) under cold stress during the early vegetative stage. Journal of Plant Growth Regulation, 36(1), 240-253.
  17. Kader, A. & Lindberg, S. (2010). Cytosolic calcium and pH signaling in plants under salinity stress. Journal of Plant Signaling and Behavior, 5(3), 233-238.
  18. Kinnersley, A.M. & Turano, F.J. )2000(. γ-aminobutyric acid (GABA) and plant responsesto stress. Critical Reviews in Plant Sciences, 19(6), 479-509.
  19. Kokabi, S., Tabatabaei, S.J. & Naghshband Hassani, R. (2012). Effects of different K/Ca ratios on the growth, yield and physiological characteristis of Galia (Cucumis melo reticulatus). Iranian Journal of Horticultural Science,42(3), 227-236. (In Farsi).
  20. Krishna, S. & Laskowski, K. (2013). Mitigation of drought stress damage by exogenous application of a non-proteinamino acid γ– aminobutyric acid on perennial ryegrass. Journal of the American Society for Horticultural Science, 138(5), 358-366.
  21. Kumar, S., & Dey, P. (2011). Effects of different mulches and irrigation methods on root growth, nutrient uptake, water-use efficiency and yield of strawberry. Scientia Horticulturae, 127(3), 318-324.‏
  22. Kurtyka, R., Malkowski, E., Kita, A. & Karcz, W. (2008). Effect of calcium and cadmium on growth andaccumulation of cadmium, calcium, potassium and sodium in maize seedlings. Polish Journal of Environmental Studies, 17(1), 51-56.
  23. Lamikanra, O. & Watson, M.A. (2004). Effect of calcium treatment temperature on fresh cutcantaloupe melon during storage. Journal of Food Science, 69(6), 468-472.
  24. L’hirondel, J. (2002). Nitrate and Man Toxic, Harmless or Beneficial Walling ford. Commonwealth Agricultural Bureaux International
  25. Levent Tun, A., Kayab, M., Ashraf, H., Altunlu, I., Yokas, C. & Yagmur, B. (2007). Theeffects of calcium sulphate on growth, membrane stability and nutrient uptake oftomato plants grown under salt stress. Environmental and Experimental Botany, 59(6), 173–178.
  26. Li, J. R., Wang, X., Tian, Z., Gao, H. B. & Wu, X. L. (2015). Effect of spraying differentconcentrations of exogenous GABA on growth and nitrogen metabolismof leek. Acta Agriculturae Zhejiangensis, 13(4), 113-119.
  27. Lichtenthaler, H.K. (1987). Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods in Enzymology, 148(34), 350-382.
  28. Luo, H.Y., Gao, H.B., Xia, Q.P., Gong, B.B. & Xiao-Lei,U. )2011(. Effects of exogenous GABA onreactive oxygen species metabolism andchlorophyll fluorescence parameters in tomatounder NaCl stress. Scientia Agricultura Sinica, 34(2), 37-54.
  29. Marschner, H. (2011). Marschner's mineral nutrition of higher plants (3rd ed.). Edited by P. Marschner. Amsterdam, Netherlands: Elsevier/Academic Press.
  30. Martin Diana, A., Rico, D., Barry-Ryan, C., Jesu, M.F., Mulcahy, J. & Gary, T.M. (2005). Comparison of calcium lactate with chlorine as a washing treatment for fresh-cut lettuce and carrots: quality and nutritional parameters. Journal of Food and Agriculture, 85(3), 2260-2268.
  31. Mumivand, H., Nushkam, A., Mohseni, A. & Babalar, M. (2013). Effect of nitrogen and calcium carbonate application rate on nitrate accumulation and yield of summer savory (Satureja hortensis L.). Journal of Crop Production, 6(3), 109-124. (In Farsi).
  32. Nasir Zadeh, F., Eigharlou, M., Khelghatibana, F. & Sadeghi, A. (2021). Nitrate and Nitrite: Sources, Impact on Human Health and Reduction of Nitrate Accumulation in Agricultural Products Using Bio fertilizers. Journal of Bio Safety, 14(2), 16-28. (In Farsi).
  33. Nayyar, H., Kaur, R., Kaur, S. & Singh, R. (2014). γ-Aminobutyric acid (GABA) imparts partial protection from heat stress injury to rice seedlings by improving leaf turgor and upregulating osmoprotectans and antioxidants. Journal of Plant Growth Regulators, 33(2), 408-419.
  34. Perucka, I. & Olszowka, K. (2011). Accumulation of potassium, magnesium, calcium in fresh and cold stored leaves of lettuce (Lactuca sativa L.) after CaCl2 foliar treatment before harvest. The Journal of Elementology, 16(3), 445-454.
  35. Pour Azar, M. R., Tabatabaei, J. & Boland Nazar, S. (2014). The effect of injection of carbon dioxide to root environment on growth and accumulation of nitrate in two lettuce varieties of screw and oven (Lactuca sativa L. cv. Capitata and sativa). Journal of Horticultural Science, 28(3), 295-302. (In Farsi).
  36. Shelp, B. J., Bozzo, G.G., Trobacher, C.P., Chiu, G. & Bajwa, V.S. (2012). Strategies and tools for studying the metabolism and function of γ-aminobutyrate in plants. International Pathway Structure Botany, 90(4), 651-668.
  37. Tabande, L. & Safarzadeh Shiraze, S. (2018). Investigation of nitrate accumulation and its effective factors in some leafy vegetables in Zanjan region. Journal of Soil Research, Soil and Water Sciences, 32(2), 189-202. (In Farsi).
  38. Tian, Z., LI, J., Wang, X., Wu, X., Gong, B. & Gao, H. (2015). Cloning of nitrate reductase gene of lettuce and effect of exogenous γ-aminobutyric acid on gene expression and nitrate content in leaves under high nitrogen level. Acta Botanica Boreali-Occidentalia Sinica, 14(6), 126-135.
  39. Wang, C.Y., Fan, L.Q., Gao, H.B., Wu, X.L., Li, J.R. & Gong, B.B. (2014). Polyamine biosynthesis and degradation are modulated by exogenous γ-aminobutyric acid in root-zone hyoxia-stressed melon roots. Journal of Plant Physiology and Biochemistry, 82(1), 17-26.
  40. Wen-bin, J., Yin-ran, D., Xi, Y. & Xiang-ning, C.H. (2013). Effect of calcium lactate treatments on physiological and biochemical changes of fresh-cut spinach. Science and Technology of Food Industry, 5(2), 2013-2019.
  41. Zahedi Pour, P. & Asghari, M.R. (2019). The effect of calcium chloride foliar application on photosynthesis and carbohydrate metabolism in strawberry seedlings. In: Proceeding of 11th Iranian Congress of Horticultural Sciences, 26-29 Aug., Urmia University, Urmia, Iran, pp. 1187-1195. (In Farsi).
  42. Zubair, H.M., Islam, M., Rahman, S., Islam, M.M., Islam, S. & Rahman, M.M. (2010). Role of GABA on growth, yield contributing characters and yield of sesame. International Journal of Bio-resource and Stress Management, 12(3), 184-188.
Iranian Journal of Horticultural Science
Volume 53, Issue 4
Winter 2023
Pages 957-966

  • Receive Date 31 January 2022
  • Revise Date 10 August 2022
  • Accept Date 31 August 2022