Effect of drought stress and foliar application of salicylic acid on some morphological ‎and physiologicalcharacters of withania (Withania coagulans Dunal.)‎

Document Type : Full Paper


1 M.Sc. Graduate, Department of Agriculture, Shirvan Branch, Islamic Azad University, Shirvan, Iran

2 Associate Professor, Crop and Horticultural Science Research Department, Khorasan Razavi Agricultural and Natural Resources Research ‎and Education Center (AREEO), Mashhad, Iran

3 Assistant Professor, Department of Agriculture, Shirvan Branch, Islamic Azad University, Shirvan, Iran


Withania is one of the most important medicinal species from economical point of view and its application in pharmaceutical industries and stabilization of sand in desert areas. This study was conducted to evaluate the effect of drought stress and foliar application of salicylic acid on some physiological and morphological characters of Withania at Khorasan Razavi Agricultural and Natural Resources Research and Education Center under green house conditions. A two factorial experiment was laid out in completely randomized design with three replications. The first factor, four levels of foliar application of salicylic acid (control, 1, 1.5, 2 mM) and the second factor, four drought levels (100, 80, 60 and 40 percent of field capacity). The results showed that drought stress and salicylic acid had significant effects (P≤1 percent) on physiological (proline content, ion leakage percent, SPAD, chlorophyll fluorescence) and morphological (shoot height and leave sourface) traits. Mean comparisons showed that the highest value of proline (3.99 µmol g-1 FW) was obtained in 1.5 mM salicylic acid and drought stress 60 percent field capacity. The highest value of ion leakage percent was obtained in the control treatment of salicylic acid and drought stress 40 percent of field capacity. According our results, revealed that the withania was tolerance drought stress and can be tolerance to 60 percent of field capacity. at all levels of drought stress. Since withania plant is valuable, salicylic acid can be increase the tolerance of this plant to drought.


  1. Abreu, I.N. & Mazzafera, P. (2005). Effect of water and temperature stress on the content of active constituents of Hypericum brasiliense Choisy. Plant Physiolology and Biochemistry, 43(3), 241-248.
  2. Ahmadi Azar, F., Hasanloo, T., Imani, A. & Feiziasl, V. (2015). Water stress and mineral zeolite application on growth and some physiological characteristics of mallow (Malva sylvestris). Journal of Plant Researches (Iranian Journal of Biology),28(3), 459-474.(In Farsi).
  3. Alkire, B.H., Simon, J.E. (1993). Water management for midwestern peppermint (Mentha piperita) growing in highly organic soel, Indiana, USA. Acta Horticulture, 344(63), 544-556.
  4. Al – Ahl, H.A.H.S. &Abdou, M.A.A. (2009). Impact of water stress and phosphorus fertilizer on fresh herb and essential oil content of drangonhead. International Agrophysics, 23, 403 - 407.
  5. Amiri Deh Ahmadi, S. R., Rezvani Moghaddam, P. & Ehyaee, H. R. (2012). The Effects of Drought Stress on morphological traits and yield of three medicinal plants (Coriandrum sativum, Foeniculum vulgare and Anethum graveolens) in Greenhouse Conditions. Iranian Journal of Field Crops Research, 10(1), 116-124. (In Farsi).
  6. Babaee, K., Amini Dehaghi, M., Modares Sanavi, S.A.M. & Jabbari, R. (2010). Water deficit effect on morphology, prolin content and thymol percentage of thyme (Thymus vulgaris). Iranian Journal of Medicinal and Aromatic Plants, 26(2), 239-251. (In Farsi).
  7. Bagheri, E., Masood sinki, J., Baradaran firozabadi, M. & Abedini sfahlani. (2013). Effect of foliar application of salicylic acid on chlorophyll fluorescence and pigment amount of sesame (Sesamum indicum) varieties under irrigated conditions. Journal of Research on Crop Ecophysiology (Agricultural Science), 7(3), 327-340. (In Farsi).
  8. Baher, Z., Mirza, M. Ghorbanli, M. & Rezaii, M.B. (2002). The influence of water stress on plant height, herbal and essential oil yield and composition in satureja hortensis Flavour and Fragrance Journal, 17(4), 275-277.
  9. Bates, L. S., Waldran, R. P. & Teare, I. D. (1973). Rapid determination of free proline for water studies. Plant and Soil, 39(1), 205-207.
  10. Belkhadi, A., Hediji, H., Abbes, Z., Nouairi, I., Barhoumi, Z., Zarrouk, M., Chaibi, W., & Djebali, W. (2010). Effects of exogenous salicylic acid pretreatment on cadmium toxicity and leaf lipid content in Linum usitatissimum L. Ecotoxicology and Environmental Safety, 73(5), 1004-1011.
  11. Cardona, C.A., Duncan, R.R. & Lindstrom, O. (1997). Low temperature tolerance assessment in paspalum. Crop Science, 37, 1283-1291.
  12. Christos, A. & Damalas, C.A. (2019). Improving drought tolerance in sweet basil (Ocimum basilicum) with salicylic acid. Scientia Horticulturae, 246, 360-365.
  13. Daneshian, J.,  Rahmani, N. &  Alimohammadi, M. (2012). Effects of application nitrogen and fertilizer manure on physiological characteristics of calendula (Calendula officinalis) under water deficit stress. New Findings in Agriculture, 6(3), 231-240. (In Farsi).
  14. Farooqi, A.H.A., Fatima, S., Ansari, S.R. & Sharma, S. (1999). Effect of water stress on growth and essential oil metabolism in cymbopogon martini (Plamerosa) cultivars. Journal of Essential Oil Research, 11, 491-496.
  15. Fazeli Kakhki, S.F., Ghiasabadi, M. & Goldani, M. (2014). Effect of salicylic acid on drought stress through improving some morphological, physiological and yield components traits of mustard plant (Brassica compestris parkland). Journal of Environmental Stresses in Crop Sciences, 7(1), 65-77. (In Farsi).
  16. Ghahremani, A., Ganji moghaddam, E., Tatari, M. & Khosroyar, S. (2020). Effect of type and concentration of growth regulators on the proliferation and marcotting of Withania coagulans medical plant. Iranian Journal of Horticultural Science,51(2), 287-294. (In Farsi).
  17. Gutierrez-Coronado, M., Trejo, C.L. & Larque-Saavedra, A. (1998). Effects of salicylic acid on the growth of roots and shoots in soybean. Plant Physiology Biochemistry, 36, 563-565.
  18. Hayat, S. & Ahmad, A. (2007). Salicylic acid: A Plant Hormone. Springer, p. 410.
  19. Isvand, H.R. & Sharafi, A. (2017). Effects of seed osmoprimage at different temperatures on emergence, seedling growth and essential oil content of Khuzestan satureja under drought stress. Journal of Seed Science and Technology of Iran, 2, 23-25. (In Farsi).
  20. Jafarzadeh, L., Omidi, H. &  Bostani, A. (2014). The study of drought stress and bio fertilizer of nitrogen on some biochemical traits of marigold medicinal plant (Calendula officinalis). Journal of Plant Researches (Iranian Journal of Biology), 27(2), 180-193. (In Farsi).
  21. Jaleel, C.A., Manivannan, P., Lakshmanan, G.M.A., Gomathinayagam, M. & Panneerselvam, R. (2008). Alterations in morphological parameters and photosynthetic pigment responses of Catharanthus roseus under soil water deficits. Colloids and Surfaces B: Biointerfaces, 61(2), 298-303.
  22. Jain, R., Kachhwaha, S., & Kothari, S.L. (2012). Phytochemistry, pharmacology and biotechnology of (Withania somnifera) and (Withania coagulans): A review. Journal of Medicinal Plants Research. 6, 5388-5399.
  23. Kabiri, R., Farahbakhs, H. & Nasibi, F. (2014). Effect of drought stress on physiological and biochemical characteristics of Nigella sativa Iranian Journal of Medicinal and Aromatic Plants, 30(4), 600 -609. (In Farsi).
  24. Karimi Afshar, A., Baghizade, A. & Mohamadinezhad, Gh. (2015). Physiological assessment of drought tolerance of two ecotypes of cumin (Cuminum cyminum) under greenhouse conditions. Journal of Science and Technology of Greenhouse Culture Soilless Culture Research Center, 6(3), 175-185. (In Farsi).
  25. Khazaie, H.R., Nadjafi, , &Bannayan, M. (2008). Effect of irrigation frequency and planting density on herbage biomass and oil production of thyme (thmus-vulgaris) and hyssop (Hyssopus officinalis). Industrial Crop and Products, 27(3), 315 - 321.
  26. Khan, W., Balakrishnan, P. &Smith, D.L. (2003). Photosynthetic responses of corn and soybean to foliar application of salicylates. Journal of Plant Physiology, 160(5), 485-492.
  27. Korkmaz, A., Korkmaz, Y. & Demirkiran, A.R. (2010). Enhancing chilling stress tolerance of pepper seedling by exogenous application of 5- aminolevolinic acid. Environmental and Experimental Botany, 67, 495-501.
  28. Maxwell, K. & Johnson, G.N. (2000). Chlorophyll fluorescence-A practical guide. Journal of Experimental Botany, 51, 659-668.
  29. Misra, A., Sricastatva, N.K. (2000). Influence of water stress on Japanese mint. Journal of Herbs, Spices & Medicinal Plants, 7, 51-58.
  30. Mohammadi Babazeidi, H.,Falaknaz, M., Heidari, P., Hemati M. S. & Farokhian, Sh. (2013). The effect of Azosprillium spp bacteria, salicylic acid and drought stress on morphological and physiological characteristics of basil (Ocimum basilicum). New Cellular and Molecular Biotechnology Journal, 3(12), 31-36. (In Farsi).
  31. Mohtashami, F., Pouryousef, M., Andalibi, B. & Shekari, F. (2015). Effects of seed priming and foliar application of salicylic acid on yield and essence of fennel (Foeniculum vulgare ) under drought stress condition. Iranian Journal of Medicinal and Aromatic Plants, 31(5), 841 -852. (In Farsi).
  32. Moosavifar, B.E., Behdani, M.A., Jami Alahmadi, M. & Hosaini Bojd, M.S. (2012). Changes of chlorophyll index (SPAD), relative water content, electrolyte leakage and seed yield in spring safflower genotypes under irrigation termination. Iranian Journal of Field Crops Research, 9(3), 525-534. (In Farsi).
  33. Moradi Marjane, E., & Goldani, M. (2011). Evaluation of different salicylic acid levels on some growth characteristics of pot marigold (Calendula officinalis) under limited irrigation. Environmental Stresses in Crop Sciences journal, 4(1), 33-45. (In Farsi).
  34. Muni Ram, D. & Singh, S. (1995). Irrigation and nitrogen requirements of bergamot mint on a sandy loam soil under sub-tropical conditions. Agricultural Water Management, 27, 45-54.
  35. Nemeth, M., Janda, T., Hovarth, E., Paldi, E. & Szali, G. (2002). Exogenous salicylic acid increases polyamine content but may decrease drought tolerance in maize. Plant Science, 162(4), 569-574.
  36. Nourzad, S., Ahmadian, A. & Moghaddam, M. (2015). Proline, total chlorophyll, carbohydrate amount and nutrients uptake in coriander (Coriandrum Sativum) under drought stress and fertilizers application. Iranian Journal of Field Crops Research, 13(1), 131-139. (In Farsi).
  37. Nonami, H., Wu, Y. .& Matthewse, M.A. (1997). Decreased growth-induced water potential a primary cause of growth inhibition at low water potentials. Plant Physiology, 114, 501-509.
  38. Pirzad, A., Alyai, H., Shakiba, M. R., Zehtab-salmasi, S. & Mohammadi, A. (2006). Eessential oil content and composition of German chamomile (Matricaria chamomilla) at different irrigation regims. Journal of Agronomy, 5(3), 451-455.
  39. Popova, L., Ananieva, V., Hristova, V., Christov, K., Geovgieva, K., Alexieva, V. & Stoinova, Z. (2003). Salicylic acid and methyl jasmonateinduced protection on photosynthesis to paraquat oxidative stress. Bulgarian Journal of Plant Physiology (Special issue), 133-152.
  40. Razavizadeh, R., Shafeghat, M. & Najafi, SH. (2014). Effect of water deficit on morphological and physiological parameters of Carum copticum. Iranian Journal of Plant Biology, 6(22), 25-38. (In Farsi).
  41. Rezapor, A.R., Heidari, M., Galavi, M. & Ramrodi, M. (2011). Effect of water stress and different amounts of sulfur fertilizer on grian yield, grain yield components and osmotic adjustment in Nigella sativa Iranian Journal of Medicinal and Aromatic Plants, 3, 384-396. (In Farsi).
  42. Sabet Teimouri, M., Kafi, M., Avarseji, Z. & Orooji, K. (2009). Investigation of drought stress levels on leaf chlorophyll content in saffron. 3th International Symposium on Saffron Biology and Technology, Kozani, Greece, 20-23 May, pp. 333.
  43. Salarpour, F. &  Farahbakhsh, H. (2016). Effects of salicylic acid on some physiological traits, yield and yield components of fennel (Foeniculum vulgare) under drought stress. Iranian Journal of Medicinal and Aromatic Plants, 32(2), 216-230. (In Farsi).
  44. Sanchez-Blanco, J., Fernandez, T., Morales, A., Morte, A. & Alarcon, J. J. (2004). Variation in water stress, gas exchange, and growth in Rosmarinus officinalis plants infected with Glamus deserticola under drought conditions. Journal of Plant Physiology, 161(6), 675-682.
  45. Shakirova, F.M., Shakhbutdinova, A.R., Bezrukova, M.V., Fatkhutdionova, R.A. & Fatkhutdionova, D.R. (2003). Changes in the hormonal status of wheat seedling induced by salicylic acid and salinity. Plant Science, 164, 317-322.
  46. Simon, J.E., Bubenheim, R.D., Joly, R.J. & Chrles, D.J. (1992). Water stress induced alternations in essential oil content and composition of sweet basil. Journal of Essential Oil Research, 4, 71-75.
  47. Tohidi Nejad,Z., Farahbakhsh, H. & Maghsoudi Moud, A.A. (2016). Evaluation of salicylic acid effects on some physiological traits of fenugreek under drought stress. Journal of Plant Process and Function Iranin Society of Plant Physiology, 5(16), 85-96. (In Farsi).
  48. Turkan, I., Bor, M., Ozdemir, F. & Koca, H. (2005). Differential responses of lipid peroxidation and antioxidant in the leaves of drought– sensitive vulgaris L. subjected to polyetylen glycol mediated water stress. Plant Science, 168, 223-231.
  49. Valizadeh, M., Bagheri, A., Valizadeh, J., Mirjalili, M.H. & Moshtaghi, N. (2015). Phytochemical investigation of Withania coagulans (Stocks) Dunal in natural habits of Sistan and Baluchestan province of Iran. Iranian Journal of Medicinal and Aromatic Plants, 31(3), 406 -417. (In Farsi).
  50. Verdonck, O. & Gabriels, R. (1992). Reference method for the determination of physical properties of plant substrates. Acta Horticulturae, 302, 169-179.
  51. Yildirim, E., Turan, M. & Guvenc, I. (2008). Applications on growth, chlorophyll and mineral content of cucumber grown under salt stress. Journal of Plant Nutrition, 31(3), 593-612.