Effect of plant growth regulators on callus induction and elicitor application for ‎limonene production in cell culture of lemon grass (Lippia citrodora)‎

Document Type : Full Paper

Authors

1 M.Sc. Student, Department of Plant Breeding and Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, ‎Iran

2 Assistant Professor, Department of Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Professor, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

Abstract

Lemon grass has been cultured in Iran due to its economic value and applications in food and pharmaceutical industries. Limonene is on  of the important compound in lemon grass essential oil. In this study, different plant growth regulators were used for callus induction in lemon grass. Root, shoot and leaf explants wee cultured on MS medium containing 2, 4-D (1, 2 and 4 mg.l-1), BAP (0.1, 0.2 and 1 mg.l-1), hormonal combination of BAP + NAA (0.5+ 0.5 mg.l-1), 2, 4-D + BAP (1+ 1 mg.l-1) and BAP + NAA (2+ 1 mg.l-1 ). The experiment was conducted as a factorial experiment in a completely randomized design with three replications. The results showed that the best treatment for increasing callus size, fresh and dry weight was BAP + NAA (0.5+ 0.5 mg.l-1 ), from leaf explants. Biotic and abiotic elicitors including MJ (50, 100 µM), SA (50, 100 µM), Tio2 ( 0.5, 2 mg.l-1) and yeast extract (0.5, 2 mg.l-1) were used for increasing limonene production in lemon grass cell culture. Used elicitors had no significant effect on limonene production in cell culture.

Keywords

References

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Iranian Journal of Horticultural Science
Volume 52, Issue 4
February 2022
Pages 889-898

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History

  • Receive Date: 23 February 2020
  • Revise Date: 13 May 2020
  • Accept Date: 01 June 2020

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