Evaluating some apple rootstocks in boron toxicity condition using morpho-‎physiological and biochemical indexes in soil

Document Type : Full Paper


1 Instructor, Seed and Plant Improvement Department, Agricultural and Natural Resources Research Center, West Azarbaijan, ‎AREEO, Urmia, Iran

2 Associate Professor, Faculty of Agriculture, Urmia University, Urmia, Iran‎

3 Assistant Professor, Soil and Water Reasearch Department., Agricultural, Education and Natural Resource Research Center of West ‎Azerbaijan, AREEO, Urmia, Iran‎

4 Professor, Faculty of Agriculture, Urmia University, Urmia, Iran‎


This trial was conducted in order to evaluating nine apple rootstocks including MM106, M26, Ottawa3, Suporter4, M9, M7, P22 , MM111 and local rootstock "Gamialmasi" in different levels boron (0, 15, 30, 45 and 60 mg/kg soil). Results indicated that height rootstock, LAI, wet and dry weight leaf, chlorophyll index, WRC and vegetative growth rate reduced in comparison control as increased boron soil in all rootstocks. Vegetative growth ceased in all rootstocks, exception M26 and P22. Also, ion leakage and MDH increased as raised boron level in soil. Ascorbate peroxidase and super oxide dismutase leaf activity increased at first and then decreased with increasing boron levels in soil. Based on leaf analysis, leaf boron concentration in M26 and P22 was less than other rootstocks. Vegetative, physiological and biochemical assessments showed that M26 and P22 were more tolerate in relation other rootstocks.


  1. Araniti, F. & Abenavoli, M R. (2016). Boron toxicity and tolerance in plants. http://www. researchgate. net/publication/301242285.
  2. Brown, P. H. & Hening, Hu. (1996). Phloem mobility of boron is species dependent: evidence for phloem mobility in sorbitol- rich species. Annals of Botany, 77, 497-505.
  3. Camacho-Cristóbal, J.J., Rexach, J. & González-Fontes, A. (2008). Boron in plants deficiency and toxicity. Plant Biology, 50, 1247-1255.
  4. Chatzissavvidis, C., Therios, I., Antonopoulou, C. & Dimassi, K. (2008). Effect of high boron concentration and scion-rootstock combination on growth and nutritional status of olive plants. Journal of Plant Nutrition, 31, 638-658.
  5. Dhindsa, R.A., Plumb-Dhindsa, P. & Thorpe, A. (1981). Leaf senescence: Correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. Journal Experimental Botany, 126, 93-101.
  6. Du, G., F. Li., Ma, F. & Liang, D. (2009). Antioxidant capacity and the relationship with polyphenol and vitamin C in actinidia fruits. Food Chemistry, 113, 557-562.
  7. El-Feky, S.S., El-Shintinawy, F.A. & Shaker, E.M., (2014). Role of CaCl2 and salicylic acid on metabolic activities and productivity of boron stressed barley (Hordeum vulgare). International Journal of Current Microbiol and Applied Scinces, 3(2), 368-380.
  8. Heath, R.L. & Packer, L. (1968). Photoperoxidation in isolated chloroplasts. Archives Biochemistry Biophysics, 125, 850-857.
  9. Herrera, R.M.B., Gonzales-Fontes, A., Rexach, J., Camacho-Cristobal, J.J.M., Maldonado, J. & Navarro-Gochicoa, M.T. (2010). Role of boron in vascular plants and response mechanisms to boron stresses. Plant Stress, 4(2), 115-122.
  10. Kamali, A. & Childers, NF. (1970). Growth and fruiting of peach in sand culture as affected by boron and fritted form of trace elements. Journal American Society for Horticultural Science, 95, 652-656.
  11. Keshavarz, P. & Malakouti, M. J. (2004). The role of boron in the balanced nutrition of plant. Sana Publication. 138 pp. (in Farsi).
  12. Kiani, A. (2011). Irrigation, basis and methods. Iran Agriculture Science Publications. (in Farsi).
  13. Koutinase, N. (2013). Respone of the apple rootstocks M9, M26 and MM106 to boron toxicity. Acta Horticulturae, 981, 471-474.
  14. Landi, M., Degl’Innocenti, E., Pardossi, A. & Guidi, L. (2012). Antioxidant and photosynthetic responses in plants under boron toxicity: a review. American Journal Agriultural and Biogical Sciences, 7, 255-270.
  15. L Lutts, S., J.M. Kinet & J. Bouharmont. (1996). NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Annals of Botany. 78: 389-398.
  16. Majidi, A. & Malakouti, M. J. (2008). Boron toxicity in Urmia lake around Orchards. Technical Leaflet. 2. Sana Publication. Tehran. (in Farsi).
  17. Majidi, A. (2011). Interaction between boron adsorbed with phosphor and silicium in calcerous soils. Ph.D. Thesis, Tarbiat Moddarres University, Tehran, Iran. (in Farsi).
  18. Molassiotis, A., Sotiropolos, T., Tanou, G., Diamanitidis, G. & Therios, I. (2006). Boron induced damage and antioxidant and nuleolytic responses in shoot tips culture of the apple rootstock EM9. Environmental and Experimental Botany, 56, 54-62.
  19. Mittler, R.(2002). Oxidative stress, antioxidants and stress tolerence. Trends in plants Science, 7(9), 405-410.
  20. Nakano, Y. & Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiology, 22, 867-880.
  21. Nable, R. O., Banuelos, G. S. & Paull, G. (1997). Boron toxicity. Journal Plant and Soil. 193, 181-198.
  22. Nezamdoost, S., Farrokhzad, A. & Rasouli-Sadaghiani, M. H. (2017). Effect of potasium silicate on reduction of boron accumulation and oxidative damages in grape (Vitis vinifera Bidaneh Sefid) under boron toxicity stress. Iranian Journal of Horticultural Science, 48, 392- 401.(in Farsi).
  23. Oraei, M., Tabatabaei, S. J., Fallahi, E. & Imani, A. (2012). The effects of boron stress on growth, physiological characteristics and the distribution of boron in scion-rootstock combination of almond (Prunus dulcis Mill). Journal of Horticultural Sciences, 26, 440-447. (in Farsi).
  24. Paparnakis, A., Chatzissavvidis, C. & Antoniadis. (2013). How apple responds to boron excess in acidic and limed soil. Journal of Soil Science and Plant Nutrition, (4), 787- 796.
  25. Rostami, H., Tabatabai, S. J., Zare Nahandi, F. & Hajiloo, J, (2013). Concentrations of boron (B) on the growth and phisiological characteristics of olives. Journal of Horticultural Science, 27, 26-18. (in Farsi).
  26. Rostami, H., Tabatabai, S.J., Zare Nahandi, F. & Poor Azar, M.R. (2014).Effects of different concentrations of boron on concentration and distribution of this element and some other nutrients in hydroponic condition in two olive cultivars. Iranian Journal of Horticultural Science,45,93-101.(in Farsi).
  27. Ruiz, J. M., Rivero, R. M., Lopez-Cantarero, I. & Romero, L. (2003). Role of Ca2+ in the metabolism of phenolic compounds in tobacco leaves (Nicotiana tabacum). Plant Growth Regulation, 41, 173.
  28. Shelp, B. J. (1988). Boron mobility and nutrition in broccoli (Brassica oleracea Italian). Annals of Botany, 61, 83-91.
  29. Tisdale, S.L. & Nelson, W.L. (1993). Soil fertility and fertilizers. Macmillan Coll Div, New York, NY. 634pp.
  30. Turner, N.C. (1981). Further progress in crop water relations. Advances in Agronomy, 58, 293-338.
  31. Wojcik, P. (2000). Availability of soil boron fractions to M26 apple rootstock. Journal of Plant Nutrition, 23(7), 1025-1035.
  32. Wojcik, P. & Treder, W. (2006). Effect of drip boron fertigation on yield and fruit quality in a high-density apple orchard. Journal of Plant Nutrition, 29, 2199-2231.
  33. Wang, J.Z., Tao, S.T., Qi, K.J., Wu, J., Wu, H.Q. & Zhang, S. L. (2011). Changes in photosynthetic properties and antioxidative system of pear leaves to boron toxicity. African Journal of Biotechnology, 10(85), 19693-19700.
  34. Wolf, B. (1974). Improvement in the azomethine-H method for the determination of boron. Communication in Soil Science and Plant Analysis, 5, 39-44.
  35. Yau, S.K. & Ryan, J. (2008). Boron toxicity tolerance in crops: a viable alternative to soil amelioration. Crop Sciences, 48(3), 854-865.