The Study of effects of spermine under salinity stress on morphophysiological ‎characteristics of Catharanthus roseus L.‎

Document Type : Full Paper


1 Assistant Professor, Faculty of Agriculture, University of Zanjan, Zanjan, Iran,‎ ‎45371-38791‎

2 M.Sc. Student, Faculty of Agriculture, University of Zanjan, Zanjan, Iran,‎ ‎45371-38791‎

3 Ph.D. Candidate, Faculty of Agriculture, University of Zanjan, Zanjan, Iran,‎ 45371-38791‎


In order to study the effects of spermine in Catharanthus roseus L under salinity stress conditions, a factorial experiment was conducted in a completely randomized design pots containing 4:1 brown peat moss to perlite in greenhouse of Zanjan University. Treatments were four spermine at four levels (0, 5, 10 and 15 ppm) and salinity stresses at three levels (0, 60 and 120 mM) of sodium chloride. Traits which were studied were as fresh and dry weight, plant height, flower diameter, leaf number as morphological traits, relative water content, total chlorophyll, carotenoids as physiological traits, activity of antioxidant enzymes such as peroxidase and proline. Results showed that application of polyamine spermine improves growth and yield of Chatarantus roseus L in stress condition and on fresh and dry weight, plant height, flower number, relative water content, proline, chlorophyll content, carotenoid, peroxidase enzyme and leaf number at 1% and 5% probability levels. The highest proline content (0.37 µmol/g FW) was obtained in 10 ppm and the lowest (0.23 µmol/g FW) in control treatment. The highest peroxidase enzyme (0.099 units. g-1 FW.min-1) was obtained in 15 ppm spermine treatment which showed an increase in spermine compared to the control. Therefore, the use of spermine in this plant for achieving desirable growth indices is recommended and can be beneficial.


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