Document Type : Full Paper
M.Sc. Student, Department of plant breeding and biotechnology, Science and Research Branch, , Islamic Azad University, Tehran, Iran
Assistant Professor, Department of plant breeding and biotechnology, Science and Research Branch, , Islamic Azad University, Tehran, Iran
Professor, Department of Agronomy and plant breeding, College of Agriculture and Natural Resources, Tehran University, Karaj, Iran
Trigonelline is one of the valuable secondary metabolites of fenugreek plant. Plant cell suspension culture is a recommended method for in vitro production of secondary metabolites. To study the possibility of Trigonelline production in fenugreek cell suspension culture, factorial experiment based on CRD was designed. In this research different explants such as leaf, stem and root were used on MS medium including four different concentration of 2,4-D and BAP plant growth regulators to study callus induction ability in fenugreek plant. The results showed that largest size of calluses belonged to the root explant under 2,4-D(2mg/l) with 315 mm and stem explant on MS including 1 mg/l BAP with 320 mm. Also, the highest fresh and dry weight of callus belonged to root explant in MS including 2 mg / l 2,4-D with 1. 5 and 0.08g and stem explant in concentration of 1 mg / l BAP with 1.5 g and 0.07g. The data registered for callus dry weight showed the same results. Three different elicitors methyl jasmonate, salicylic acid and yeast extract were used to increase the in vitro production of Trigonelline using cell suspension culture obtained from Fenugreek calluses. The highest amounts of Trigonelline was obtained from 100 μM salicylic acid.